Separation and purification method for lactococcus acidophilus antibacterial peptide

A Lactobacillus acidophilus, separation and purification technology, applied in the field of separation and purification of antimicrobial peptides, can solve problems such as protein denaturation

Inactive Publication Date: 2016-05-11
源耀生物科技(盐城)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The conventional extraction method of Lactobacillus acidophilus antimicrobial peptides is to purify by salting out or organic solvent precipitation followed by column chromatography. transsexual

Method used

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Embodiment Construction

[0015] The content of the present invention will be further described in detail below.

[0016] A method for separating and purifying Lactobacillus acidophilus antimicrobial peptides, comprising the following steps: (1) Solid fermentation of Lactobacillus acidophilus to obtain a Lactobacillus acidophilus fermentation product; (2) Dissolving the Lactobacillus acidophilus fermentation product in water, Take the supernatant and pass through multiple ultrafiltration membranes of different specifications to obtain components with different molecular weights; (3) freeze-dry the supernatant of each component, and measure the antibacterial activity of each component; (4) through coagulation Gel column chromatography obtained high-purity Lactobacillus acidophilus antimicrobial peptide; (5) Thermal stability test of Lactobacillus acidophilus antibacterial peptide. Further preferably, the fermentation temperature in step (1) is 36° C. to 37° C., the anaerobic fermentation is carried out,...

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Abstract

The invention discloses a separation and purification method for lactococcus acidophilus antibacterial peptide. The method comprises the following steps that 1, a lactococcus acidophilus solid is fermented to obtain a lactococcus acidophilus fermentation product; 2, after the lactococcus acidophilus fermentation product is dissolved into water, liquid supernatant is taken and passes through multiple ultra-filtration membranes of different specifications to obtain components with different molecular weights; 3, the liquid supernatant of each component is dried in a freezing mode, and bacteriostatic activity of all the components is tested; 4, gel column chromatography is carried out on the component with the best acteriostatic activity; 5, thermal stability of lactococcus acidophilus antibacterial peptide is tested. The ultra-filtration membranes are adopted for carrying out filtering according to the different molecular weights of the substances, impure protein can be removed, the property of protein is not influenced, and the method is suitable for large-scale production of industry.

Description

technical field [0001] The invention relates to a method for separating and purifying antimicrobial peptides. Background technique [0002] At present, in the food, medicine and animal husbandry industries, due to the extensive use and abuse of traditional antibiotics, a large number of drug-resistant strains and drug residues have emerged, posing a great threat to human health. Moreover, with the improvement of people's health consumption awareness, there is an urgent need to develop new natural antibacterial substances that are not easy to produce drug resistance, have no residue, and have no potential safety hazards. Therefore, as a natural source of protein antimicrobial peptides, it has become a research hotspot in recent years, and has a wide range of applications in food, medicine and animal husbandry industries. Antimicrobial peptides are mainly derived from insects, mammals, amphibians, plants, bacteria, etc. [0003] Lactobacillus acidophilus is one of the main p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08C07K14/335C07K1/36C07K1/34C07K1/16
CPCC07K7/06C07K1/16C07K1/34C07K1/36C07K7/08C07K14/335
Inventor 李世超方华季春源朱校适郭子好
Owner 源耀生物科技(盐城)股份有限公司
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