Dietary supplement containing rich water-insoluble glucan and preparation method of dietary supplement
A dietary supplement, water-insoluble technology, applied in the fields of bacteria, food science, and food ingredients used in food preparation, can solve the problems of strong satiety and lack of dietary supplements, and achieve improved satiety and high food Safety, the effect of improving safety
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Embodiment 1
[0028] Example 1 Preparation of a water-insoluble glucan-enriched dietary supplement
[0029] 1. Preparation of dietary supplement enriched with water-insoluble glucan
[0030] Preparation of tomato juice sucrose medium: Ripe tomatoes were washed, peeled, squeezed by a juicer, filtered with 80-mesh gauze to extract the juice, boiled for 5 minutes, centrifuged at 8000g for 10 minutes to take the supernatant, added 10% (w / v) sucrose to heat and dissolve, and then cooled. to room temperature with Na 2 CO 3 Adjust pH to 7, sterilize at 121° C. for 20 min, and cool to room temperature to obtain sterile tomato juice sucrose medium.
[0031] Preparation of seeds (fermentative strains): The lyophilized powder of Leuconostocmesenteroides BD3749 was dissolved with a small amount of sterile distilled water, and a loop was taken with an inoculation loop and streaked on M17 containing 2% (w / v) sucrose. On agar medium (purchased from MerckCo. Germany), take out after 24h aerobic culture ...
Embodiment 2
[0050] Example 2 Preparation of a water-insoluble glucan-enriched dietary supplement
[0051] The preparation method of seeds (fermentation strains), the preparation method of water-insoluble glucan in fermentation liquid, and the preparation method of water-insoluble glucan in tomato juice fermented beverage are the same as those in Example 1.
[0052] Preparation of tomato juice sucrose medium: ripe tomatoes are washed, peeled, squeezed by a juicer, filtered with 100-mesh gauze to extract the juice, boiled for 10 minutes, centrifuged at 4000g for 12 minutes to take the supernatant, added with 15% sucrose, heated and dissolved, and then cooled to room temperature, with NaHCO 3 Adjust pH to 8, sterilize at 135°C for 10 min, and cool to room temperature to obtain sterile tomato juice sucrose medium.
[0053] (1) Aseptically inoculate the obtained Leuconostoc BD3749 seeds with an inoculation amount of 5% (v / v) in a tomato juice sucrose medium with a sucrose mass percentage cont...
Embodiment 3
[0056] Example 3 Preparation of dietary supplement enriched with water-insoluble glucan
[0057] The preparation method of seeds (fermentation strains), the preparation method of water-insoluble glucan in fermentation liquid, and the preparation method of water-insoluble glucan in tomato juice fermented beverage are the same as those in Example 1.
[0058] Preparation of tomato juice sucrose culture medium: ripe tomatoes are washed, peeled, squeezed by a juicer, filtered with 120-mesh gauze to extract the juice, boiled for 1 min, centrifuged at 12000g for 8 min to take the supernatant, add 5% sucrose by mass, heat and dissolve, and then cool to At room temperature, the pH was adjusted to 5 with NaOH, sterilized at 110° C. for 30 min, and cooled to room temperature to obtain a sterile tomato juice sucrose medium.
[0059] (1) Aseptically inoculate the obtained Leuconostoc BD3749 seeds with an inoculation amount of 1% (v / v) in a tomato juice sucrose medium with a sucrose mass pe...
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