Identification method of stephania epigaea medicinal material
A technology of jinbuhuan and medicinal materials, applied in the field of quality inspection of jinbuhuan medicinal materials, can solve problems such as shortage, quality control of jinbuhuan, and slow development of artificial cultivation
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Embodiment 1
[0023] Establishment of Standard Fingerprints
[0024] 1 Instruments and reagents
[0025] 1.1 Instrument
[0026] High performance liquid chromatography: Shimadzu LC-20A, equipped with UV detector, chromatographic workstation, Japan Shimadzu; ultrapure water manufacturing system: Chengdu Yuechun Technology Co., Ltd.; CNC ultrasonic cleaner: KQ-500DB, Kunshan City Ultrasonic Instrument Co., Ltd.; Electric Heating Constant Temperature Blast Drying Oven: 101-1-S, Shanghai Yuejin Medical Instrument Factory; Low Temperature Coolant Circulating Pump: DLSB-5 / 20, Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.; Circulating Water Vacuum Pump: SHZ -D(III), Gongyi Yingyu Yuhua Instrument Factory; Electric constant temperature water bath: GSY-n stainless steel, Beijing Medical Equipment Factory; Vacuum drying oven: D2F-250, Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.; electronic analytical balance : ME215S, Beijing Sartorius Instrument System Co., Ltd.; mult...
Embodiment 2
[0058] The determination method of the fingerprint of Jinbuhuan reference medicinal material is the same as that of the standard fingerprint of Jinbuhuan medicinal material, and the sampling time is 60 minutes. The standard fingerprint and common pattern of Jinbuhuan medicinal material obtained in Example 1 were used as the basis for identification, and the differences between the two were compared. The results showed that the fingerprints of the Jinbuhuan control medicinal materials provided by the Food and Drug Inspection Institute of Guangxi District ( image 3 ) is not significantly different from the standard fingerprint, see Table 5.
[0059]
Embodiment 3
[0061] The determination method of commercially available Jinbuhuan is the same as the determination method of Jinbuhuan standard fingerprint, and the sampling time is 60 minutes. The standard fingerprint and common pattern of Jinbuhuan medicinal material obtained in Example 1 were used as the basis for identification, and the differences between the two were compared. The result shows: commercially available Jinbuchang fingerprints (see Figure 4 ) is quite different from the Jinbuhuan standard fingerprint, in which the number of main peaks, the relative retention time of the main peaks, and the relative peak area are all quite different, and there are many common peaks that do not appear, see Table 6.
[0062]
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