Megakaryotic progenitor cell cryopreservation medium and its application

A technology of megakaryotic progenitor cells and cryopreservation solution, which is applied in the field of bioengineering and can solve the problems of lack of cryopreservation research on megakaryotic progenitor cell types

Active Publication Date: 2018-10-26
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of research on cryopreservation for the specific cell type of megakaryotic progenitor cells

Method used

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  • Megakaryotic progenitor cell cryopreservation medium and its application
  • Megakaryotic progenitor cell cryopreservation medium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The inventor adopts 7 kinds of cryopreservation solution containing only DMSO and culture medium, commercially available 07930 cryopreservation solution, plasma-free cryopreservation solution containing HSA, and plasma-containing cryopreservation solution of the present invention, respectively. The megakaryotic progenitor cells induced and expanded in vitro were cryopreserved, and the cryopreservation effect was compared to observe the effects of umbilical cord plasma and poloxamer on the cryopreservation of megakaryotic progenitor cells, as follows:

[0035] 1. Experimental method

[0036](1) Mononuclear cells from umbilical cord blood were isolated, megakaryotic progenitor cells were induced and expanded for 10 days in vitro, and expanded megakaryotic progenitor cells were obtained.

[0037] (2) The megakaryotic progenitor cells obtained on the 10th day of above-mentioned in vitro induction and expansion were cryopreserved using seven kinds of cryopreservation solutio...

Embodiment 2

[0045] The megakaryotic progenitor cells were cryopreserved according to the method of Example 1, except that the frozen cells were megakaryotic progenitor cells induced in vitro for 14 days, and the components of some cryopreservation solutions were different. The specific components of the cryopreservation solutions are shown in Table 2.

[0046] Table 2 Effects of different cryopreservation solutions on the cryopreservation effect of megakaryotic progenitor cells induced in vitro for 14 days (n=3)

[0047]

[0048] The results showed that plasma can effectively improve the survival rate of cryopreservation solution, and the number of recovered cells is large, and within a certain range, with the increase of plasma concentration, the survival rate of cells and the number of recovered cells also increased. Moreover, P188 and P407 can further increase the survival rate of cells and the number of recovered cells to a certain extent.

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Abstract

Disclosed are a megakaryocyte progenitor cryopreservation liquid and the use thereof, wherein the megakaryocyte progenitor cryopreservation liquid comprises: DMSO and plasma. The cryopreservation liquid can be effectively used to cryopreserve megakaryocyte progenitors, and the survival rate and recovery rate after cell revival are high.

Description

technical field [0001] The present invention relates to the field of bioengineering, in particular, to a cryopreservation liquid for megakaryotic progenitor cells and applications thereof, more specifically, to a cryopreservation liquid for megakaryotic progenitor cells, a kit for cryopreserving megakaryotic progenitor cells, and a method for cryopreserving megakaryotic progenitor cells method. Background technique [0002] In recent years, the incidence of tumors has been on the rise. Thrombocytopenia is often found in the blood tests of patients undergoing radiotherapy and chemotherapy. Due to the shortage of blood sources and the need for repeated transfusions of platelets, it is necessary to find new sources of platelets. Umbilical cord blood hematopoietic stem / progenitor cells can differentiate into megakaryotic progenitor cells, and further mature to produce platelets. Therefore, it is expected to increase the number of platelets in the blood by infusing megakaryotic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/02
Inventor 裴雪涛陈琳刘大庆岳文李雪潘志婷黄安平陈志清吕洋
Owner ACADEMY OF MILITARY MEDICAL SCI
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