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Moringa oleifera extract for promoting intestinal growth and preparation method and application thereof

A kind of Moringa oleifera extract, technology of use, applied in the field of medicine

Inactive Publication Date: 2016-06-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But so far, there is no research report that moringa can improve intestinal health and promote intestinal growth through reasonable moringa nutrition therapy for patients with intestinal failure or short bowel syndrome

Method used

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  • Moringa oleifera extract for promoting intestinal growth and preparation method and application thereof
  • Moringa oleifera extract for promoting intestinal growth and preparation method and application thereof
  • Moringa oleifera extract for promoting intestinal growth and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing a Moringa oleifera extract, comprising the steps of: soaking Moringa oleifera oleifera with 75% ethanol by mass percentage for 2 hours, then heating and refluxing with 75% ethanol by mass percentage for extraction twice, each extraction The time is 3 hours, the ethanol soaking solution and the ethanol extract are combined, then the extract is extracted with ethyl acetate, and the layers are separated to obtain the ethyl acetate extract; the ethyl acetate extract is concentrated, and then loaded on Silica gel chromatography column, first elute with petroleum ether-acetone system of 50:1 (volume ratio), then elute with methanol, collect the methanol eluate; after the methanol eluate is concentrated, load the sample on D101 macroporous adsorption resin, eluted with 95% ethanol by mass percentage, collected ethanol eluate, concentrated and dried to obtain Moringa oleifera extract.

Embodiment 2

[0029] A method for preparing a Moringa oleifera extract, comprising the steps of: soaking Moringa oleifera oleifera with 95% ethanol by mass percentage for 5 hours, and then heating and refluxing with 795% ethanol for 5 times of extraction, each extraction The time is 1 to 5 hours, the ethanol soaking solution and the ethanol extract are combined, and then the extract is extracted with ethyl acetate, and the layers are separated to obtain the ethyl acetate extract; the ethyl acetate extract is concentrated, and then the The sample is placed on a silica gel chromatography column, and firstly eluted with a petroleum ether-acetone system of 60:1 (volume ratio), and then eluted with methanol, and the methanol eluate is collected; after the methanol eluate is concentrated, the upper Take the sample in D101 macroporous adsorption resin, elute with 95% ethanol by mass percentage, collect the ethanol eluate, concentrate, and dry to obtain Moringa oleifera extract.

Embodiment 3

[0031] A method for preparing a Moringa oleifera extract, comprising the steps of: soaking Moringa oleifera oleifera with 70% ethanol by mass percentage for 1 hour, and then extracting once by heating and refluxing with 70% ethanol by mass percentage, the extraction time For 1 hour, combine the ethanol soaking solution and the ethanol extracting solution, then extract the extracting solution with ethyl acetate, and separate layers to obtain the ethyl acetate extracting solution; concentrate the ethyl acetate extracting solution, and then apply the sample to the silica gel layer Column analysis, eluting with 40:1 (volume ratio) petroleum ether-acetone system first, then eluting with methanol, collecting methanol eluate; after concentrating the methanol eluate, load the sample on D101 Pore ​​adsorption resin, eluting with 70%% ethanol by mass percentage, collecting ethanol eluate, concentrating and drying to obtain Moringa oleifera extract.

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Abstract

The invention discloses a moringa oleifera extract for promoting intestinal growth and a preparation method and application thereof. The preparation method includes the following steps of extracting moringa oleifera through ethyl alcohol, and extracting and layering an extraction solution through ethyl acetate to obtain an ethyl acetate extraction solution; concentrating the ethyl acetate extraction solution, putting a sample on a silica gel chromatographic column, conducting elution through a petroleum ether-acetone system, conducting elution through methyl alcohol, and collecting a methyl alcohol elution solution; putting a sample on large-pore adsorption resin after concentrating the methyl alcohol elution solution, conducting elution through ethyl alcohol, collecting an ethyl alcohol elution solution, and conducting concentration and drying to obtain the moringa oleifera extract. The moringa oleifera extract has the advantage that intestinal growth and intestinal villus growth can be effectively promoted.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a Moringa oleifera extract for promoting intestinal growth, a preparation method and application thereof. Background technique [0002] The word "intestinal failure" appeared in the literature in the 1950s and is still used today, but it does not have a complete meaning, nor does it have clear monitoring parameters like other organs. In fact, the word "Failure" is used in dictionaries at home and abroad. In , its meaning mainly refers to failure, failure and decline, and does not necessarily mean that the function is irreversible. Before the 1980s, it was believed that the main function of the intestinal tract was digestion and absorption. Therefore, the intestinal function monitoring index is mainly the degree of digestion and absorption. After the 1980s, the importance of intestinal mucosal barrier function has been recognized in clinical work, and intestinal bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P1/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55
Inventor 田洋盛军董扬郝淑美解静陈玮史崇颖彭磊徐安妮戴天浥
Owner YUNNAN AGRICULTURAL UNIVERSITY
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