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Rapid ELISA (enzyme-linked immunosorbent assay) method for leather hydrolyzed protein L-hydroxyproline in raw milk

The technology of leather hydrolyzed protein and hydroxyproline is applied in the field of enzyme label rapid detection of leather hydrolyzed protein L-hydroxyproline in raw milk, which can solve the problems of complicated operation and high toxicity of detection reagents, and achieves good repeatability. , the effect of fast detection and high sensitivity

Inactive Publication Date: 2016-06-08
WEINAN HUALONG ANIMAL HUSBANDRY CO LTD
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Problems solved by technology

Colorimetric detection reagents are highly toxic and relatively cumbersome to operate

Method used

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  • Rapid ELISA (enzyme-linked immunosorbent assay) method for leather hydrolyzed protein L-hydroxyproline in raw milk
  • Rapid ELISA (enzyme-linked immunosorbent assay) method for leather hydrolyzed protein L-hydroxyproline in raw milk
  • Rapid ELISA (enzyme-linked immunosorbent assay) method for leather hydrolyzed protein L-hydroxyproline in raw milk

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Embodiment Construction

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0035] figure 1 It exemplarily shows a schematic flow chart of an enzyme-labeled rapid detection method for L-hydroxyproline in leather hydrolyzed protein in fresh milk provided by the embodiment of the present invention. This method can be applied at least to L-hydroxyproline in milk and dairy products. Determination of proline content and determination of whether animal hydrolyzed protein is added to milk and dairy products. This is because L-hydroxyproline...

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Abstract

The invention discloses a rapid ELISA (enzyme-linked immunosorbent assay) method for leather hydrolyzed protein L-hydroxyproline in raw milk. The method comprises the following steps: 1, reagent preparation; 2, sample and standard preparation; 3, ELISA; 4, establishment of a standard curve and result calculation. The ELISA method has high sensitivity, good reproducibility and high detection speed and is applicable to rapid screening of the leather hydrolyzed protein in the raw milk. Further, time consumed by the detection method is shortened by more than two thirds while compared with time consumed by a method assigned by the Ministry of Health, and the reagent dose is almost 3% of the reagent dose of the assigned method, so that the method has the advantages of rapid detection, low cost and little environmental pollution.

Description

technical field [0001] The invention relates to the technical field of fresh milk detection, and more specifically relates to an enzyme-labeled rapid detection method for leather hydrolyzed protein L-hydroxyproline in fresh milk. Background technique [0002] L-Hydroxyproline, English name hydroxyproline, HYP for short, also known as trans-4-hydroxyl-L-proline, molecular formula C5H9NO3, molecular weight 131.13. [0003] L-Hydroxyproline is an imino acid, usually with a hydroxyl group in the 4th position of proline, but sometimes also in the 3rd position. L-Hydroxyproline is an amino acid unique to collagen, which accounts for about 10% of the total amino acids in collagen, and is very small in other proteins, which makes it almost a unique component of collagen, so it can be passed through The content of L-hydroxyproline is measured to calculate the content of collagen, so as to determine the overall level of collagen. [0004] Animal collagen hydrolyzed protein is a chea...

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Application Information

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IPC IPC(8): G01N21/31G01N21/78G01N1/28
CPCG01N21/31G01N1/28G01N21/78
Inventor 赵彩会李胜雷浩田间贾青李宏赵佳苗李会玲陈争上王军良李明涛冯西辉
Owner WEINAN HUALONG ANIMAL HUSBANDRY CO LTD
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