Method for detecting ancient wool fabrics by aid of fluoroimmunoassay

A technology of immunofluorescence and detection method, which is applied in the detection field of ancient wool fabrics by immunofluorescence, can solve the problems such as the inapplicability of woolen fabrics cultural relics, and achieves the effects of high sensitivity, simple operation and avoiding interference.

Inactive Publication Date: 2016-06-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above method is only suitable for the detection of wool ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A detection method utilizing immunofluorescence to measure ancient woolen fabrics, adopts the following steps:

[0021] Raw material preparation:

[0022] PBS buffer solution of pH7.4: Weigh KCl0.2g, KH 2 PO 4 0.27g, NaCl8.0g, Na 2 HPO 4 1.42g, dissolved in 800mL distilled water and adjusted to 1000mL, adjusted to pH 7.4.

[0023] Buffered glycerol: weigh Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800mL of distilled water and adjusted to 1000mL, adjusted to pH 9.6; PBS buffer with pH 9.6 and glycerol were mixed at a volume ratio of 1:1.

[0024] Rabbit anti-wool keratin antibody diluent: Dilute the rabbit anti-wool keratin antibody with an initial concentration of 1 mg / mL by 100 times with bovine serum albumin solution with a mass concentration of 1%.

[0025] Green fluorescein-labeled goat anti-rabbit IgG (H+L) antibody diluent: Add 0.15 mg of green fluorescent microspheres and 2.7 μL of 1- (3-Dimethylaminopropyl)-3-ethylcarbodiimide solution and 10 μL of go...

Embodiment 2

[0031] A detection method utilizing immunofluorescence to measure ancient woolen fabrics, adopts the following steps:

[0032] Raw material preparation:

[0033] PBS buffer solution of pH7.4: Weigh KCl0.2g, KH 2 PO 4 0.27g, NaCl8.0g, Na 2 HPO 4 1.42g, dissolved in 800mL distilled water and adjusted to 1000mL, adjusted to pH 7.4.

[0034]Buffered glycerol: weigh Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800mL of distilled water and adjusted to 1000mL, adjusted to pH 9.6; PBS buffer with pH 9.6 and glycerol were mixed at a volume ratio of 1:1.

[0035] Rabbit anti-wool keratin antibody diluent: Dilute the rabbit anti-wool keratin antibody with an initial concentration of 1 mg / mL 10 times with bovine serum albumin solution with a mass concentration of 1%.

[0036] Green fluorescein-labeled goat anti-rabbit IgG (H+L) antibody diluent: Add 0.14 mg of green fluorescent microspheres and 2.6 μL of 1 -(3-Dimethylaminopropyl)-3-ethylcarbodiimide solution and 8 μL of goat ant...

Embodiment 3

[0042] A detection method utilizing immunofluorescence to measure ancient woolen fabrics, adopts the following steps:

[0043] Raw material preparation:

[0044] PBS buffer solution of pH7.4: Weigh KCl0.2g, KH 2 PO 4 0.27g, NaCl8.0g, Na 2 HPO 4 1.42g, dissolved in 800mL distilled water and adjusted to 1000mL, adjusted to pH 7.4.

[0045] Buffered glycerol: weigh Na 2 CO 3 0.15g, NaHCO 3 0.29g, dissolved in 800mL of distilled water and adjusted to 1000mL, adjusted to pH 9.6; PBS buffer with pH 9.6 and glycerol were mixed at a volume ratio of 1:1.

[0046] Rabbit anti-wool keratin antibody diluent: Dilute the rabbit anti-wool keratin antibody with an initial concentration of 1 mg / mL by 200 times with bovine serum albumin solution with a mass concentration of 1%.

[0047] Green fluorescein-labeled goat anti-rabbit IgG (H+L) antibody diluent: Add 0.16 mg of green fluorescent microspheres and 2.8 μL of 1 -(3-Dimethylaminopropyl)-3-ethylcarbodiimide solution and 12 μL of go...

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Abstract

The invention relates to the field of technologies for detecting antiques, and discloses a method for detecting ancient wool fabrics by the aid of fluoroimmunoassay. The method includes A), arranging three antique samples in three perforated vessels A, B and C, washing the antique samples by the aid of PBS (phosphate buffer solution), adding rabbit anti-wool-keratin antibody diluent into the perforated vessel A, adding PBS into the perforated vessel C, refrigerating the perforated vessels, then adding PBS into the various perforated vessels and soaking and washing the antique samples; B), adding goat anti-rabbit IgG (H+L) antibody diluent with green fluorescein markers into the perforated vessels A and B, adding PBS into the perforated vessel C, inoculating the perforated vessels A, B and C in the absence of light, then adding PBS in the various perforated vessels and soaking and washing the antique samples; C), adding buffer glycerin into the perforated vessels A, B and C and sealing slices; D), observing and detecting the perforated vessels A, B and C under laser confocal microscopes. The method has the advantages of high sensitivity, simplicity in operation, low cost, visual results and easiness in analysis.

Description

technical field [0001] The invention relates to the technical field of cultural relic detection, in particular to a detection method for detecting ancient woolen fabrics by using immunofluorescence. Background technique [0002] Wool fabric is the main textile raw material in the ancient northern region and is a precious heritage of China. However, due to the environmental factors in the northern region and the nature of wool protein fibers, most of the decayed cultural relics have been turned into traces. How to use scientific means to identify wool has become an urgent need. Commonly used detection methods are greatly affected by samples, impurities, etc., and the accuracy of the results is not high, so a simpler and more intuitive scientific method is urgently needed. [0003] The Chinese patent with the application number 201010577180.1 discloses a method for analyzing the content of wool fiber, cashmere and wool blended fiber by physical method, which uses fiber photog...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/68
CPCG01N33/533G01N33/68G01N2333/4742
Inventor 游秋实王秉刘杨刘意
Owner ZHEJIANG SCI-TECH UNIV
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