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Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain

A strain and pyridine technology, applied in the field of environmental biology, can solve the problems of inhibiting microbial growth and metabolism, poor degradation ability of pyridine-degrading bacteria, rapid reduction of ammonia nitrogen concentration in wastewater, etc., and achieve the effect of strong pyridine degradation ability

Inactive Publication Date: 2016-06-29
WUHAN UNIV OF SCI & TECH
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Problems solved by technology

[0004] The pyridine-degrading bacteria will produce the metabolite ammonia in the process of degrading pyridine, and the accumulation of ammonia will produce feedback inhibition on the degradation of pyridine, thereby significantly reducing the pyridine-degrading ability of the bacteria, which is why the degradation ability of the currently screened pyridine-degrading bacteria is not strong one of the important reasons
Moreover, ammonia nitrogen itself is an environmental pollutant that widely exists in industrial, agricultural and domestic wastewater. If it diffuses into the environment, it will cause great harm. It is one of the main causes of eutrophication in water bodies. Ammonia nitrogen can significantly inhibit the growth and metabolism of microorganisms, and also have a significant negative impact on the biodegradation of pyridine and other pollutants and the bioremediation of the environment. However, most of the ammonia nitrogen degrading bacteria currently screened and applied to ammonia nitrogen wastewater treatment have weak degradation ability , cannot quickly reduce the concentration of ammonia nitrogen in wastewater in a short period of time

Method used

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  • Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain
  • Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain
  • Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and Identification of Pyridine Degrading Bacteria

[0026] (1) Domestication and screening of pyridine degrading bacteria

[0027] The strain CZ3 in this example was obtained by sampling from the aeration tank and the secondary sedimentation tank of the coking wastewater biochemical treatment workshop of Wuhan Iron and Steel Company, and was obtained through enrichment, primary screening and secondary screening.

[0028] The composition of the LB liquid medium in the embodiment: yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L. LB solid medium is 1.8% agar added to LB liquid medium.

[0029] The composition of MSP inorganic salt medium is as follows: Na 2 HPO 4 6.78g / L, KH 2 PO 4 3.0g / L, NaCl0.5g / L, MgSO 4 ·7H 2 O0.5g / L, CaCl 2 0.011g / L, pyridine 500-3000mg / L, trace elements 2ml / L. Trace element composition: MnSO 4 ·H 2 O1.69g / L, CoCl 2 ·6H 2 O0.24g / L, H 3 BO 3 1.16g / L, Na 2 MoO 4 2H 2 O0.024g / L, FeSO4 7H2O2.78g / L, ZnSO4 7H2O1.15g / L, ...

Embodiment 2

[0041] Application of Example 2 bacterial strain CZ3 in pyridine wastewater treatment

[0042] The composition of the slant medium in this example: 5 g / L of yeast extract, 10 g / L of tryptone, 10 g / L of NaCl, 50 μg / mL of kalamycin, and 1.8 g% of agar. LB liquid culture medium formula is the same as embodiment 1. Seed medium composition: yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L, pyridine 1500mg / L. The MSP inorganic salt medium containing different concentrations of pyridine was used as the pyridine-containing simulated wastewater, and the composition of the MSP medium was the same as in Example 1.

[0043] (1) Seed cultivation

[0044] ①Pick the colonies on the slant and transfer them to 2‐5mL LB liquid medium, and cultivate them to the logarithmic phase at 30°C and 180rpm.

[0045] ② Inoculate 3% of the bacterial liquid cultivated in step ① into the seed medium, and cultivate it to the logarithmic phase at 30°C and 180 rpm as the seed liquid.

[0046] (2) Degradation ...

Embodiment 3

[0049] Application of Example 3 bacterial strain CZ3 in the biological treatment of ammonia nitrogen wastewater

[0050] In this embodiment, the composition of the slant medium is the same as that of Example 2, and the composition of the LB liquid medium is the same as that of Example 1. Seed medium composition: yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L, NH 4 Cl8g / L. Composition of simulated wastewater containing ammonia nitrogen: Na 2 HPO 4 6.8g / L, KH 2 PO 4 3.0g / L, NaCl0.5g / L, MgSO 4 7H2O0.5g / L, CaCl 2 0.01g / L, NH 4 Cl1.0g / L, glucose 4.0g / L, NH 4 Cl0.4‐15g / L, trace elements 2mL / L, pH7.0. Trace element composition: MnSO 4 ·H 2 O1.69g / L, CoCl 2 ·6H 2 O0.24g / L, H 3 BO 3 1.16g / L, Na 2 MoO 4 2H 2 O0.024g / L, FeSO4 7H2O2.78g / L, ZnSO4 7H2O1.15g / L, CuSO 4 ·5H 2 O0.38g / L.

[0051] seed culture

[0052] ①Pick the colonies on the slant and transfer them to 2‐5mL LB liquid medium, and cultivate them to the logarithmic phase at 30°C and 180rpm.

[0053] ② Inocu...

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Abstract

The invention discloses a bacterial strain capable of degrading pyridine and ammonia nitrogen. The bacterial strain is characterized by being preserved in CCTCC (China Center for Type Culture Collection) on April 25th, 2014, the preservation number is CCTCC M2014165, and the bacterial strain belongs to arthrobacter sp. and is named as Arthrobacter ureafaciens CZ3. The bacterial strain has efficient degradation capacity on high-concentration pyridine and has very high endurance capacity and efficient removal capacity on the environmental pollutant ammonia nitrogen, does not produce secondary pollution and is safe to use. The bacterial strain has broad application prospect and very high popularization value in the aspects of treatment of industrial wastewater containing high-concentration pyridine and biological treatment of wastewater containing high-concentration ammonia nitrogen.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to a bacterial strain capable of efficiently degrading pyridine and ammonia nitrogen, a preparation method and an application thereof. Background technique [0002] As an industrial solvent and a basic chemical raw material for the synthesis of various compounds, pyridine is used in pesticides, medicines, dyes, daily chemicals, spices, feed additives, rubber additives and other fields. Pyridine is a typical nitrogen-containing heterocyclic compound. It is easily soluble in water, easily diffuses into the environment, and is difficult to biodegrade. It seriously threatens human health and belongs to the "three hazards" environmental pollutants. [0003] Compared with traditional physical and chemical treatment methods, bioremediation has the advantages of high efficiency, less investment, low operating costs, less secondary pollution, and ecological tolerance. I...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/06C02F101/38
CPCC02F3/34C02F2101/38C02F2209/14C12N1/20C12N1/36C12N1/205C12R2001/06
Inventor 周卫吴晓琴杨忠华左振宇侯亚利
Owner WUHAN UNIV OF SCI & TECH
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