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Dry-type immunofluorescence kit for detecting Alzheimer syndrome MS4A6A and preparation method thereof

A MS4A6A, Alzheimer's technology, applied in the field of medical products, to achieve the effect of convenient measurement, simple operation, and low background value

Inactive Publication Date: 2016-07-13
上海凯璟生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even though they are expressed in different cell types, MS4A family members may participate in some functions to a large extent because of the conservation of gene loci and the identity in structure and sequence.

Method used

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  • Dry-type immunofluorescence kit for detecting Alzheimer syndrome MS4A6A and preparation method thereof
  • Dry-type immunofluorescence kit for detecting Alzheimer syndrome MS4A6A and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of dry immunofluorescence kit for Alzheimer's syndrome MS4A6A of the present invention

[0018] The kit of the present invention comprises a PVC base plate, a fluorescent binding pad, a nitrocellulose membrane, a sample pad and a water-absorbing pad; the sample pad, the nitrocellulose membrane, the fluorescent binding pad, and the water-absorbing pad are fixed on the PVC base plate in a horizontal direction.

[0019] 1. Preparation of nitrocellulose membrane (NC membrane)-PVC bottom plate

[0020] 1. Scribing film: Cut the nitrocellulose into 25mm×300mm and paste it on the PVC bottom plate. Dilute the rabbit anti-chicken IgY with PBS to a concentration of 1 mg / ml as the C-line coating solution; dilute the mouse anti-human MS4A6A monoclonal antibody 2 with PBS to a concentration of 1 mg / ml as the T-line coating solution. Using a film sprayer, streak the T-line coating solution and C-line coating solution on the nitrocellulose membrane to obtain the...

Embodiment 2

[0029] Embodiment 2 The using method of kit of the present invention

[0030] 1. Use a pipette to pipette 50ul serum, plasma, and cerebrospinal fluid samples and add them to the sample pad of the test strip;

[0031] 2. Stand at room temperature for 10 minutes;

[0032] 3. At the end of 10 minutes, put the test strip into the immunofluorescence quantitative analyzer to read the data;

[0033] 4. The immunofluorescence quantitative analyzer measures and analyzes the optical signal, and quantitatively obtains the concentration of the tested substance;

[0034] 5. Judgment of negative and positive according to the reference value.

Embodiment 3

[0035] Embodiment 3 kit of the present invention compares with ELISA kit

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Abstract

The invention relates to a dry-type immunofluorescence kit for detecting Alzheimer syndrome MS4A6A and a preparation method thereof. The kit comprises a PVC (polyvinyl chloride) bottom plate, a fluorescent-binding pad, a nitrocellulose membrane, a sample pad and a water absorbent pad, wherein the sample pad, the nitrocellulose membrane, the fluorescent-binding pad and the water absorbent pad are fixed on the PVC bottom plate horizontally; a coated mouse anti-human MS4A6A monoclonal antibody 1-nano-fluorescence bead compound and a coated chicken IgY-nano-fluorescence bead compound are arranged on the fluorescent-binding pad, and a detection line formed by mouse anti-human MS4A6A monoclonal antibody 2 and a quality control line formed by rabbit anti-chicken IgY antibody are formed on the nitrocellulose membrane. According to the kit, the nano-fluorescence bead is used for detecting MS4A6A, the background value is low, the detection time is short, the specificity and the sensitivity are high, the detection result is accurate, and the kit and the method provide a more reliable basis for clinical diagnosis and Alzheimer syndrome.

Description

technical field [0001] The invention relates to a dry immunofluorescence kit and a preparation method for detecting Alzheimer's syndrome MS4A6A, and the invention belongs to the field of medical products. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease with insidious onset and progressive development. AD is one of the most common diseases in the elderly. The World Health Organization (WHO) estimates that the prevalence of AD in the elderly population over 65 years old is 4% to 7%. The prevalence of AD is closely related to age. For every 6.1 years of age on average, the prevalence doubles; among the elderly over 85 years old, the prevalence of AD can be as high as 20% to 30%. In 2001, there were more than 20 million AD patients worldwide, and it is estimated that by 2040, there will be more than 80 million AD patients worldwide. Since 1999, China has entered an aging society. Correspondingly, the current population of AD patients in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/543
CPCG01N33/6896G01N33/54346G01N33/558G01N33/577G01N2333/47G01N2800/2821
Inventor 罗朝领董敏卢荣春
Owner 上海凯璟生物科技股份有限公司
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