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Laccase composite dielectric body and decolorization method

A composite mediator and mediator technology, applied in the field of dye liquor decolorization, can solve the problems of difficult degradation of dyes, high price of synthetic mediators, inactivation of laccase, etc., and achieve the effect of improving decolorization speed and decolorization rate

Inactive Publication Date: 2016-07-20
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic mediators are expensive, highly toxic, and easy to inactivate laccase, while natural mediators are relatively low-toxic, easy to obtain, cheap, and difficult to inactivate laccase
However, due to the refractory nature of dyes, the decolorization efficiency is not ideal if only natural mediators or synthetic mediators are used alone

Method used

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  • Laccase composite dielectric body and decolorization method
  • Laccase composite dielectric body and decolorization method
  • Laccase composite dielectric body and decolorization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Composite mediator to the decolorization experiment of neutral red (anthraquinone dyes)

[0026] (1) Reagent and concentration:

[0027] Neutral red: 40uM,

[0028] CotA: 25mU / ml,

[0029] ABTS: 40uM,

[0030] AS: 40uM

[0031] Neutral Na 2 HPO 4 -citric acid buffer

[0032] The molar ratio of AS to ABTS is 1 and AS+ABTS=40uM, reaction system: 1ml.

[0033] (2) Experimental steps

[0034] Take 5 Ep tubes with the same specifications, add 40uM neutral red, and number them 1-5 respectively; add 25mU / ml CotA laccase to tube 2, add 40uMABTS to tube 3 on the basis of tube 2, and tube 4 to tube 2 Add 40uMAS on the basis of tube 5, add AS / ABTS=1 and AS+ABTS=40uM on the basis of tube 2, use Na with a pH of 7 2 HPO 4 -The citricacid buffer was filled up to 1ml; the specific experimental conditions for 5 test tubes were as follows:

[0035] Test tube number The amount of CotA laccase added ABTS added amount AS addition amount 1 - - - ...

Embodiment 2

[0041] Embodiment 2: the influence of the composite mediator of different proportioning on decolorization effect

[0042] (1) Reagent and concentration:

[0043] Neutral red: 40uM,

[0044] CotA: 25mU / ml,

[0045] ABTS: 40uM,

[0046] AS: 40uM

[0047] Neutral Na 2 HPO 4 -citric acid buffer

[0048] The molar ratio of AS to ABTS is 0.1-9:1 and AS+ABTS=40uM, reaction system: 1ml.

[0049] (2) Experimental steps:

[0050] Take 8 Ep tubes with the same specifications, add 40uM neutral red and 25mU / ml CotA laccase, and number 1-8 respectively; add AS / ABTS=0.1 mixed mediator and ABTS+AS=40uM to No. 1 tube, No. 2 Add AS / ABTS=0.3 mixed mediator and ABTS+AS=40uM, tube No.3 adds AS / ABTS=0.5 mixed mediator and ABTS+AS=40uM, tube No.4 adds AS / ABTS=1 mixed mediator And ABTS+AS=40uM, add AS / ABTS=2 mixed mediator on No. 5 and ABTS+AS=40uM, add AS / ABTS=3 mixed mediator on No. 6 and ABTS+AS=40uM, add AS / ABTS=4 mixed mediator and ABTS+AS=40uM, add AS / ABTS=9 mixed mediator and ABTS+A...

Embodiment 3

[0056] Embodiment 3: Composite mediator is to the decolorization experiment of acid blue 74 (indigo dyes)

[0057] (1) Reagent and concentration:

[0058] Acid Blue 74:40uM,

[0059] CotA: 25mU / ml,

[0060] ABTS: 20uM,

[0061] AS:20uM

[0062] Neutral Na 2 HPO 4 -citric acid buffer

[0063] The molar ratio of AS to ABTS is 1 and AS+ABTS=20uM, the reaction system: 1ml.

[0064] (2) Experimental steps:

[0065] Take 5 Ep tubes with the same specifications, add 40uM acid blue 74, and number 1-5 respectively; add 25mU / ml CotA laccase to tube 2, add 20uMABTS to tube 3 on the basis of tube 2, and tube 4 to tube 2 Add 20uMAS on the basis of the tube, add AS / ABTS=1 and AS+ABTS=20uM on the basis of No. 2 tube, use pH7Na 2 HPO 4 -The citricacid buffer was replenished to 1ml; 5 tubes were placed on a shaker at 40°C and 130rpm at the same time for reaction; the spectrophotometer was used to test at 608nm at 0.5h, 1h, and 1.5h respectively. The experimental results are as follow...

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Abstract

The invention discloses a laccase composite dielectric body composed of two dielectric bodies: acetosyringone AS and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, wherein the AS / ABTS molar ratio is (0.1-9) to 1. The composite dielectric body is formed from the acetosyringone AS and the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS; in a decolorization process, the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS can be subjected to concerted reaction with the acetosyringone, so as to improve the decolorization speed and decolorization rate of laccase.

Description

technical field [0001] The invention relates to the technical field of dye liquor decolorization, in particular to a laccase composite mediator and a decolorization method using the composite mediator. Background technique [0002] Laccase is a copper-containing polyphenol oxidase in the same subfamily as ascorbate oxidase in plants and ceruloplasmin in mammals in the family of bluecopperoxidases. According to statistics, there are more than 250 kinds of catalyzed substrates of laccase, mainly including phenols, aromatic amines, carboxylic acids, metal organic compounds, some steroid hormones and biological pigments. Due to the wide range of substrates, laccase has great application potential in many fields such as food industry, wastewater treatment, pulp bleaching, soil purification, dye decolorization, organic synthesis, wood processing, and immunoassay. [0003] Dye has become inseparable from life, but the removal of dye is still a big problem. At present, dyes can be...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCC02F3/342C02F2101/308C02F2305/00
Inventor 王刚刚罗爽谢天刘忠川
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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