Colloidal gold immunochromatographic test strip for aided detection of pancreatic cancer and preparation method of golloidal gold immunochromatographic test strip

Abstract

The invention provides a colloidal gold immunochromatographic test strip for aided detection of pancreatic cancer and a preparation method of the golloidal gold immunochromatographic test strip. The strip comprises a bottom plate, a sample absorbent pad, a colloidal gold conjugate pad, a nitrocellulose membrane and a water absorption pad; when the strip is prepared, the sample absorbent pad, the colloidal gold conjugate pad, the nitrocellulose membrane and the water absorption pad are sequentially adhered to the bottom plate in an overlapped manner; the colloidal gold conjugate pad is coated with colloidal gold-labeled CA19-9 monoclonal antibody and colloidal gold-labeled monoclonal antibody of helicobacter pylori hemagglutinin antigen; the nitrocellulose membrane is coated with a detection line 1, a detection line 2 and a quality control line; the detection line 1 is coated with colloidal gold-labeled CA19-9 monoclonal antibody; the detection line 2 1 is coated with colloidal gold-labeled monoclonal antibody of helicobacter pylori hemagglutinin antigen; the quality control line is coated with goat anti-rabbit IgG antibody. The strip is simple in preparation and operation steps and high in sensitivity, and provides a good basis for early examination of pancreatic cancer.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a colloidal gold immunochromatographic test paper for auxiliary detection of pancreatic cancer and a preparation method thereof. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat. About 90% of them are ductal adenocarcinomas originating from the ductal epithelium. The morbidity and mortality have increased significantly in recent years. The rate is <1%, and it is one of the malignant tumors with the worst prognosis. The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low. The incidence of this disease is higher in men than in women, with a male to female ratio of 1.5 to 2:1. Male patients are far more common than premenopausal women, and the incidence of postmenopausal women is similar to that of men. The etiol...

AI Technical Summary

Problems solved by technology

The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low