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New application of tor protein inhibitor to inhibit Fusarium wilt and Verticillium dahliae

A protein inhibitor, the technology of Fusarium wilt, applied in the direction of plant growth regulator, preservation of human or animal body, botanical equipment and methods, etc., to reduce the use of pesticides, reduce the use of pesticides, and have broad prospects.

Inactive Publication Date: 2018-06-05
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TOR protein inhibitors are currently mainly used in clinical research, and some are also used in the study of plant growth and development and the regulation mechanism of metabolic pathways. However, there are no related reports on the prevention and treatment of plant diseases by inhibiting the activity of fungal TOR protein.

Method used

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  • New application of tor protein inhibitor to inhibit Fusarium wilt and Verticillium dahliae
  • New application of tor protein inhibitor to inhibit Fusarium wilt and Verticillium dahliae
  • New application of tor protein inhibitor to inhibit Fusarium wilt and Verticillium dahliae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1.Rapamycin, Torin1 and Afrost Hymexazol deal with cotton fusarium wilt and cotton verticillium dahliae

[0038] Preparation of fungal spore suspension: Fusarium wilt and Verticillium dahliae were cultured with PDA solid medium. Use a puncher to take a piece of culture medium containing Fusarium wilt and Verticillium dahliae into sterile 100mL PDA liquid medium for culture, culture at 25°C and 200rpm for 2-3 days, and then filter with four layers of sterile gauze , just can get pure cotton fusarium wilt and Verticillium dahliae spore suspension.

[0039] 1. Treat cotton Fusarium wilt and Verticillium dahliae respectively with Rapamycin

[0040] Set different Rapamycin (RAP) concentration gradients to deal with Fusarium wilt and Verticillium dahliae:

[0041] The concentration of Rapamycin to treat Fusarium wilt of cotton is 0.1 μmol / L, 0.5 μmol / L and 1 μmol / L;

[0042] The concentration of Rapamycin to treat Verticillium dahliae was 1nmol / L, 5nmol / L and 10...

Embodiment 2

[0054] Embodiment 2.Rapamycin+Torin1 combined drug treatment cotton Fusarium wilt and Verticillium dahliae

[0055] 1. Treatment of Fusarium wilt of cotton with Rapamycin+Torin1 combination medicine

[0056] The concentrations of Rapamycin were: 1nmol / L, 10nmol / L, 50nmol / L; the concentrations of Torin1 were: 1μmol / L, 5μmol / L. Combining different concentrations of Rapamycin and Torin1 in pairs gave 6 combinations which were used to treat Fusarium wilt. The PDA medium, the method of adding Rapamycin+Torin1, and the method of treating Fusarium wilt of cotton are the same as in Example 1, except that the bacteria inhibitor is Rapamycin+Torin1, and it is a combination of different concentrations.

[0057] 2. Treatment of Verticillium dahliae in cotton with Rapamycin+Torin1 combination drug

[0058] The concentrations of Rapamycin were: 1nmol / L, 2nmol / L, 5nmol / L; the concentrations of Torin1 were: 5μmol / L, 10μmol / L. Two combinations of Rapamycin and Torin1 with different concentr...

Embodiment 3

[0061] Example 3. Rapamycin+Metalax · Hymexazol Combination Medication Treatment of Cotton Fusarium wilt and Verticillium dahliae

[0062] Cotton Fusarium wilt and Verticillium dahliae were treated with the combination of Rapamycin+Metalax·Hymexazol according to the method of Example 2.

[0063] When dealing with Fusarium wilt of cotton, the concentrations of Rapamycin were: 10nmol / L, 50nmol / L, 100nmol / L; the concentrations of armoram·hymexazol were: 0.05mL / L, 0.1mL / L. Combining different concentrations of Rapamycin and metalaxe·hymexazine in pairs gave 6 combinations to deal with Fusarium wilt.

[0064] When dealing with Verticillium dahliae of cotton, the concentrations of Rapamycin were: 0.5nmol / L, 1nmol / L, 2nmol / L; the concentrations of metalapam·hymexazol were: 10μL / L, 20μL / L. Combine different concentrations of Rapamycin and metalaxe·hymexazol in pairs to get 6 combinations to deal with Verticillium dahliae respectively.

[0065] Observation of results: After cultivati...

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Abstract

The invention discloses application of a TOR protein inhibitor to inhibition of wilt fusarium and verticillium dahliae of plants and a fungus bactericide. The fungus bactericide contains the TOR protein inhibitor, wherein the TOR protein inhibitor is Rapamycin, Torin 1, a mixture of Rapamycin and Torin 1, or a mixture of Rapamycin and a fungus chemical bactericide. According to the application disclosed by the invention, the TOR protein inhibitor used for clinical medicine is used for prevention and treatment researches of the wilt fusarium and the verticillium dahliae of the plants for the first time; researches show that the TOR protein inhibitor including Rapamycin, Torin 1, the mixture of Rapamycin and Torin 1 and the mixture of Rapamycin and the fungus chemical bactericide can be used for effectively inhibiting the wilt fusarium and the verticillium dahliae of the plants, so that a new choice for preventing and treating wilt and verticillium wilt is provided, and has very important meanings on reducing the utilization of pesticides and delaying the insect resistance.

Description

technical field [0001] The invention relates to a new application of a TOR protein inhibitor, in particular to a new application of a TOR protein inhibitor for inhibiting plant Fusarium wilt and Verticillium dahliae. Background technique [0002] Fusarium wilt is a typical vascular disease with sudden onset and symptoms including severe spotting, wilting, or death of the entire plant. Fusarium oxysporum is the causative agent of Fusarium wilt, which belongs to the subphylum Idiomycetes and the genus Fusarium. Fusarium wilt is a worldwide distribution of soil-borne pathogenic fungi with a wide range of hosts, which can cause more than 100 plant wilts in melons, Solanaceae, bananas, cotton, legumes and flowers. Verticillium dahliae is a serious vascular disease. Its pathogenic bacteria are mainly Verticillium dahliae Kleb and Verticillium alboatrum. Fungus. Because Verticillium dahliae has a wide range of hosts and has strong adaptability to high temperature and severe temp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N43/80A01N37/46A01P3/00
CPCA01N37/46A01N43/80A01N43/90A01N2300/00
Inventor 任茂智李林宣冯丽李正国
Owner CHONGQING UNIV
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