The invention relates to an indirect competition
enzyme linked immunoreagent kit for detecting mercury ions and a manufacturing method of the kit. An elisa plate enveloped by mercury
ion envelope antigens, mercury-
ion-resisting
monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a mercury
ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The kit can detect the mercury irons in a trace mode, is used for detecting the mercury irons in the environment, the soil, water, foods, medicines,
cosmetics and the like, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform
field detection. The kit is low in requirement for pretreating samples, simple in treatment process, not only can be used for screening the samples in large batch, but also can perform
rapid detection on the samples in small batch, and not only provides
technical support for environment and
food safety, but also provides effective technological means for food import and export inspection,
food inspection, monitoring and evaluation of environmental
pollution and the like.