Indirect competitive enzyme linked immunosorbent assay kit for detecting chromium ions as well as preparation and detection methods thereof

Abstract

The invention relates to an enzyme-linked immunoassay kit for detecting chromium ions and its assembly and detection method. The kit contains an ELISA plate coated with trivalent chromium ion-coated antigen, anti-trivalent chromium ion monoclonal antibody, enzyme-labeled secondary antibody, substrate chromogenic solution, stop solution, and trivalent chromium ion standard solution , lotion concentrate, sample treatment solution. Sample processing is carried out before detection, so that Cr3+ in the sample forms Cr3+-EDTA, and then the kit is used for detection. The chromium ion ELISA kit of the present invention can detect trace amounts of chromium ions, and the sensitivity of detecting Cr3+ is 2ng / mL. Operation error; the detection cost is less than 1 / 20 of the physical and chemical analysis method, and the timeliness is strong, and on-site detection can be carried out. It is mainly used for the screening of large batches of samples with chromium ion contamination residues in the environment, soil, water, and food.

Description

technical field [0001] The invention relates to the fields of ELISA and chromium ion detection, in particular to an ELISA kit for detecting chromium ions and its assembly and detection method. Background technique [0002] Chromium is an element that widely exists in nature, distributed in soil, air, water and organisms, mainly in the form of trivalent chromium (Cr 3+ ) and hexavalent chromium (Cr 6+ ) exists in the form of Chromium is one of the essential trace elements for humans and animals, Cr 3+ Has the activity of glucose tolerance factor (GTF), participates in the regulation of cholesterol and glucose metabolism in organisms, Cr 3+ Lack of it will cause fat deposition on the inner wall of blood vessels in the body and lead to arteriosclerosis. But Cr 3+ and Cr 6+ Another toxic substance, Cr 6+ The toxicity is Cr 3+ 100 times more toxic, many studies have confirmed that Cr 3+ Carcinogenic and mutagenic; Cr 6+ Carcinogenic toxicity, mucous membrane toxicity, l...

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Problems solved by technology

In August 2011, the Qujing chromium slag pollution incident, the hexavalent chromium content detected in the groundwater outlet exceeded the standard by more than 200 times; in April 2012, the chromium-excessive medicinal capsule incident caused economic losses of more than 16 billion yuan

[0004] At present, the established food chromium detection techniques mainly include graphite furnace atomic absorption spectrometry (GFAAS), flame atomic absorption spectrometry (FAAS), inductively coupled plasma mass spectrometry (ICP-MS), and inductively coupled plasma atomic emission spectrometry. (ICP-AES), hydride generation-atomic fluorescence spectrometry (HG-AFS), electrochemical analysis methods, etc., but these physical and chemical analysis methods require expensive equipment and skilled professionals, and the detection is cumbersome, time-consuming, and the cycle is long. High, cannot be operated on site, and the amount of sample screening is small, etc., its application is limited

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