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Reagent for detecting clostridium symbiosum and application thereof

A symbiotic Clostridium and reagent technology, applied in the field of disease diagnosis, can solve the problems of expensive, unsuitable screening and follow-up for the general public, and time-consuming problems, and achieve low cost, reduce missed diagnosis rate, and improve sensitivity and specificity Effect

Inactive Publication Date: 2016-07-27
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

Although this method has high specificity, it is very expensive and time-consuming, and is not suitable for screening and follow-up of the general public
Therefore, so far there is no cheap, simple and non-invasive method for early diagnosis of colorectal cancer with high sensitivity and specificity.

Method used

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  • Reagent for detecting clostridium symbiosum and application thereof
  • Reagent for detecting clostridium symbiosum and application thereof
  • Reagent for detecting clostridium symbiosum and application thereof

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Embodiment Construction

[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0029] 1. Patient and tissue samples:

[0030] 1) Population: Stool samples from patients who underwent digital colonoscopy and colorectal cancer surgery from January 1, 2012 to December 30, 2015 at Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine.

[0031] The inclusion criteria are:

[0032] (1) Age ≥ 50 years old;

[0033](2) Have a normal colonic movement rhythm, and the frequency of defecation is no more than twice a day, and no less than once a day;

[0034] (3) Full colonoscopy was performed by experienced doctors in accordance with standard specifications, and the time for withdrawal was sufficient.

[0035] The exclusion criteria are:

[0036] (1) Previous history of colorectal adenoma, colorectal cancer, irritable bowel syndrome (IBS), ulcerative colitis (UC), or Crohn's disease (CD);

[0037] (2) Previous hist...

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Abstract

The invention discloses an application of the reagent for detecting clostridium symbiosum in preparing an early colorectal cancer diagnostic kit. According to the diagnostic kit, DNA of bacteria in excrement is extracted and a quantitative polymerase chain reaction is conducted so that the feature of relative abundance of the clostridium symbiosum can be acquired, and diagnosis of early colorectal cancers (limited to the colon cancer and the rectal cancer of submucosa) is conducted with the feature as a basis. Compared with noninvasive colorectal cancer screening methods applied to clinics at present and applied for a patent, the regent is completely noninvasive, operation is relatively easy and low in price, the colorectal cancers can be predicted more accurately, and the area under curve (AUC value) of an ROC curve is 0.70 or above.

Description

technical field [0001] The invention relates to the field of disease diagnosis, in particular to a reagent for detecting symbiotic Clostridium in feces and its application in early diagnosis of colorectal cancer. Background technique [0002] Colorectal cancer ranks third in the incidence of all tumors. Early detection and intervention of colorectal cancer can effectively improve the prognosis. At present, the early diagnosis of colorectal cancer relies on colonoscopy biopsy. However, colonoscopy biopsy is an invasive examination and cannot be used for large-scale population screening. An ideal tumor screening index should have high sensitivity and specificity, and at the same time be as non-invasive as possible. Research progress in recent years suggests that there are significant differences in the distribution of intestinal flora between patients with colorectal cancer and normal healthy people, and intestinal flora may be involved in the occurrence and development of c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/10C12N15/11C12R1/145
CPCC12Q1/689C12Q1/6886
Inventor 房静远许杰谢元鸿
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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