The invention provides a phosphatidylserine-containing composition as well as a preparation method and application thereof. The phosphatidylserine-containing composition contains the following ingredients in parts by weight: 5-40 parts of phosphatidylserine, 10-30 parts of DHA (Docosahexaenoic Acid) algae oil and 10-40 parts of fructus alpiniae oxyphyllae. According to the phosphatidylserine-containing composition provided by the application, a 'PS (phosphatidylserine)-DHA' combination is adopted, the phosphatidylserine (PS), the DHA algae oil and the fructus alpiniae oxyphyllae which are selected and used according to a certain proportion are taken as main raw materials, and through the combination of Chinese traditional and Western medicine, the three drugs have an effective synergistic effect, and have the efficacy of improving memory and enhancing brain functions. According to the preparation method, the phosphatidylserine-containing composition can be prepared into tablets, granules or soft capsules, has the advantages of convenient operation, simple process and the like, and is suitable for volume production and large-scale applications.