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Pollution-resistance nitragin and preparation method thereof

A rhizobia agent and anti-pollution technology, which is applied in the field of anti-pollution rhizobia agent and its production, can solve the problems of inconvenient large-scale promotion and application, difficulty in forming a dominant community of rhizobia, high production equipment and technology, etc., to achieve The mutagenesis method is simple and easy, inhibits the indigenous rhizobia, and is easy to obtain the effect

Inactive Publication Date: 2011-01-05
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have strict requirements on the aseptic conditions of production, high requirements on production equipment and technology, high production cost of bacterial agents, difficult to be accepted by small and medium-sized bacterial agent manufacturers, and inconvenient for large-scale promotion and application
[0006] (2) Competition between the rhizobia strains in the rhizobia agent and the native rhizobia: Since the native rhizobia are fully adapted to the local soil environment and have strong competitiveness, it is difficult for the newly inoculated rhizobia to form a dominant community, making the nodulation rate low and efficient The ratio of nitrogen-fixing root nodules is reduced, and the effect of increasing yield is limited and weakened
However, due to the variety of rhizobia strains and the variety of crop habitats, it is impossible to genetically improve the corresponding rhizobia strains for each crop in each habitat
Therefore, it has been difficult to use in actual production for many years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] By using microwave irradiation (800W, 1min, 200ml bacterial liquid, bacterial liquid 600nm light absorption value is 0.5) to process the fermented bacterial liquid of Rhizobium Onobrychis vicilfolia (Rhizobium Onobrychis vicilfolia) and Rhizobium meliloti (Rhizobium meliloti) with high efficiency, the processed The rhizobium bacterium and rhizobium meliloti bacterium liquid after are coated on the YMA substratum containing ampicillin 300mg / L and 400mg / L respectively, will carry out the rhizobia strain that can normally grow on the drug-containing medium ) plant back grafting test to determine its nodulation ability and growth-promoting effect. Passage tests were carried out on mutant strains whose nodulation ability and growth-promoting effect were stronger than those of the starting strain, and finally selected strains with genetically stable drug-resistant characteristics were used as antibiotic-resistant rhizobia strains for production;

[0041] The mutant strains of...

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Abstract

The invention discloses a pollution-resistance nitragin comprising zymophyte fluid of an antibiotics-resistance rhizobium strain and the corresponding antibiotics, wherein the final concentration of the corresponding antibiotics in the zymophyte fluid is 100 to 250mg / L. The invention simultaneously discloses a preparation method of the pollution-resistance nitragin, which comprises the steps of irradiation mutagenesis of rhizobium, screening of drug-resistance mutants, production of the pollution-resistance nitragin and the like. The pollution-resistance nitragin in the invention can ensure that the defects of high contamination rate of the nitragin, low competitiveness of target strains and native rhizobium and the like can be overcome to realize the purpose of effectively reducing the preservation and transportation cost of the nitragin while reducing the contamination rate of the nitragin, improving the strain competitiveness and enhancing effective viable counts.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to an anti-pollution rhizobia agent and a preparation method thereof. Background technique [0002] At present, rhizobia agents, as microbial growth-promoting fertilizers for leguminous crops and forage grasses, have been widely used in agricultural production. They are non-toxic, harmless, and do not pollute the environment. The yield-increasing effect of reclamation or low-yielding fields can reach 71.2%-241%. Both at home and abroad are actively researching and applying. The production and application of microbial fertilizers in my country has a history of nearly 50 years. In recent years, not only the scale of production has been expanded and the quantity has increased, but also some new varieties and dosage forms have been developed. [0003] But, in the process of realizing the present invention, the designer finds that the main problems faced in the production and application of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N15/01C12Q1/04C12R1/41
Inventor 师尚礼李剑峰曹文侠张淑卿刘建荣祁娟
Owner GANSU AGRI UNIV
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