Pollution-resistance nitragin and preparation method thereof

Abstract

The invention discloses a pollution-resistance nitragin comprising zymophyte fluid of an antibiotics-resistance rhizobium strain and the corresponding antibiotics, wherein the final concentration of the corresponding antibiotics in the zymophyte fluid is 100 to 250mg/L. The invention simultaneously discloses a preparation method of the pollution-resistance nitragin, which comprises the steps of irradiation mutagenesis of rhizobium, screening of drug-resistance mutants, production of the pollution-resistance nitragin and the like. The pollution-resistance nitragin in the invention can ensure that the defects of high contamination rate of the nitragin, low competitiveness of target strains and native rhizobium and the like can be overcome to realize the purpose of effectively reducing the preservation and transportation cost of the nitragin while reducing the contamination rate of the nitragin, improving the strain competitiveness and enhancing effective viable counts.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to an anti-pollution rhizobia agent and a preparation method thereof. Background technique [0002] At present, rhizobia agents, as microbial growth-promoting fertilizers for leguminous crops and forage grasses, have been widely used in agricultural production. They are non-toxic, harmless, and do not pollute the environment. The yield-increasing effect of reclamation or low-yielding fields can reach 71.2%-241%. Both at home and abroad are actively researching and applying. The production and application of microbial fertilizers in my country has a history of nearly 50 years. In recent years, not only the scale of production has been expanded and the quantity has increased, but also some new varieties and dosage forms have been developed. [0003] But, in the process of realizing the present invention, the designer finds that the main problems faced in the production and application of p...

AI Technical Summary

Problems solved by technology

These methods have strict requirements on the aseptic conditions of production, high requirements on production equipment and technology, high production cost of bacterial agents, difficult to be accepted by small and medium-sized bacterial agent manufacturers, and inconvenient for large-scale promotion and application

[0006] (2) Competition between the rhizobia strains in the rhizobia agent and the native rhizobia: Since the native rhizobia are fully adapted to the local soil environment and have strong competitiveness, it is difficult for the newly inoculated rhizobia to form a dominant community, making the nodulation rate low and efficient The ratio of nitrogen-fixing root nodules is reduced, and the effect of increasing yield is limited and weakened

However, due to the variety of rhizobia strains and the variety of crop habitats, it is impossible to genetically improve the corresponding rhizobia strains for each crop in each habitat

Therefore, it has been difficult to use in actual production for many years