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Method for improving germination of medicago sativa seeds under acid-aluminum stress

An alfalfa and seed technology, applied in the directions of seed immunity, seed and rhizome treatment, application, etc., can solve the problems of long cycle, difficult to adapt to the needs of the promotion and planting of alfalfa and the development of animal husbandry, etc., and achieve the effect of solving the low germination rate.

Inactive Publication Date: 2016-08-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It takes a long time to improve the aluminum tolerance of alfalfa by breeding methods, and it is difficult to adapt to the needs of alfalfa in the acidic soil in the south and the development of animal husbandry in the short term.

Method used

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  • Method for improving germination of medicago sativa seeds under acid-aluminum stress
  • Method for improving germination of medicago sativa seeds under acid-aluminum stress
  • Method for improving germination of medicago sativa seeds under acid-aluminum stress

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Melatonin treatment in an artificially controlled environment improves the germination of alfalfa seeds under acid aluminum stress. The main steps are as follows:

[0025] 1. Disinfect alfalfa seeds

[0026] The commercial alfalfa cultivar 'Juneng 995' was used as the material. Soak the alfalfa seeds in a beaker filled with 0.15% potassium permanganate solution for 5 minutes, then remove the seeds, filter them dry, rinse them with distilled water for 3 times, and then dry them in the air.

[0027] 2. Determination of optimal concentration and soaking time

[0028] In order to determine the optimal concentration and soaking time of the melatonin solution, set the melatonin solution to five concentration gradients of 0, 5, 10, 20 and 40 μmol / L, and set the soaking time to 0, 4, 8 and 12 h. After soaking the seeds, remove and filter dry; lay two layers of filter paper in a petri dish, soak the filter paper with a solution with a pH value of 4.5 and containing 100 μmol / L ...

Embodiment 2

[0033] The test of melatonin treatment improving the germination of alfalfa seeds under acid aluminum stress in the natural environment of the field, the operation steps are as follows:

[0034] 1. Disinfect alfalfa seeds

[0035] The commercial alfalfa cultivar 'Juneng 995' was used as the material. Soak the alfalfa seeds in a beaker filled with 0.15% potassium permanganate solution for 5 minutes, then remove the seeds, filter them dry, rinse them with distilled water for 3 times, and then dry them in the air.

[0036] 2. Soak the seeds with melatonin solution

[0037] Make melatonin into an optimal solution concentration of 20 μmol / L, soak the alfalfa seeds for 8 hours at room temperature and avoid light, then remove and filter to dry;

[0038] 3. Sowing:

[0039] The experimental site is yellow soil with a pH value of 5.5-6.0. Deep plow the ground before sowing, apply superphosphate 35kg / mu and ammonium bicarbonate 45 kg / mu as base fertilizer, then use mechanical rotary...

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Abstract

The invention discloses a method for improving germination of medicago sativa seeds under acid-aluminum stress and relates to the technical field of production and management forage grass. The method comprises the following steps: (1) disinfecting the medicago sativa seeds, namely immersing the medicago sativa seeds into a 0.15% potassium permanganate solution under a room-temperature condition for disinfecting for 5 minutes; (2) immersing the seeds in a melatonin solution, namely immersing the medicago sativa seeds under a room-temperature dark condition for 8 hours; and (3) promoting the germination of the seeds, namely culturing the seeds under the conditions that the temperature is 25+ / -1 DEG C and the seeds are illuminated for 10 hours and then are put into a dark place for 14 hours alternately for 10 days to promote the germination of the seeds. Melatonin used by the invention is an amine type hormone and has no toxin and harms; the dosage of the melatonin is less when the melatonin is used for treating the seeds so that the treatment is economical; the method is simple and feasible, has rapid effect and is strong in operability; the germination quality of the medicago sativa seeds can be improved in short time; the problem that the germination rate of the medicago sativa seeds in acidic soil is low can be effectively solved; and technical supports are provided for popularizing and planting medicago sativa in Southern acid soil.

Description

technical field [0001] The invention relates to the technical field of forage production and management, in particular to a method for improving the germination of alfalfa seeds under acid aluminum stress. Background technique [0002] Acidic soil refers to the soil with a pH value lower than 7, and for agricultural production, the soil pH value of the plow layer is lower than 6.6 is acidic soil. Acidic soil accounts for 40% of the world's arable land, and accounts for about 21% of the land area in my country. It is widely distributed in the southern red-yellow soil area, and the area of ​​acidic soil is increasing with the deterioration of the environment. Aluminum is the most abundant metal element in the earth's crust, accounting for about 7% of the mass of the earth's crust. Under natural conditions, it exists mainly as aluminosilicates with little bioavailability. In recent years, due to the use of agricultural chemical fertilizers and the impact of acid rain, the soi...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08
CPCA01C1/00A01C1/08
Inventor 胡龙兴蔡璐杨知建向佐湘
Owner HUNAN AGRICULTURAL UNIV
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