Nanochannel for detecting antigen-antibody specific binding, preparation method and detection method thereof

Abstract

The invention provides a nanochannel with a nanocage structure. The nanochannel comprises a top SiN film layer, a middle SiO2 interlayer and a bottom SiN film layer which form a sandwich structure, wherein a small nanopore with area slightly larger than the maximum sectional area of antigen to be detected and smaller than the minimum sectional area of antibody to be detected is machined on the top SiN film layer, a large nanopore with diameter larger than that of the small nanopore is machined on the bottom SiN film layer and located at a position aligned with the small nanopore in the axial direction, and SiO2 between the small nanopore and the large nanopore is corroded into a hollow nanocage structure; two liquid bath units filled with ionic solution are connected to the two ends of the nanochannel with a nanocage structure, and the antigen and the antibody are added into the same liquid bath next to the small nanohole. When ionic current change is detected under both forward and reverse voltage, it is concluded that specific binding of the antigen and the antibody is not achieved. When ionic current change is detected under forward voltage only, it is concluded that specific binding of the antigen and the antibody is achieved, and then identifying detection of antigen or antibody molecules can be achieved.

Description

technical field [0001] The invention relates to the preparation of a nanochannel with a novel nanocage structure, by comparing the ion current blocking signal caused by a single antigen molecule passing through the nanochannel and the collision signal caused when the antigen / antibody conjugate moves to the vicinity of the nanopore , to achieve the purpose of detection and identification of a certain antigen / antibody specific binding. Background technique [0002] Currently, solid-state nanopores are mainly 3 N 4 , SiO 2 、Al 2 o 3 , graphene, MoS 2 prepared on thin films. The shape of the prepared nanopore can be divided into two types: one is the nanopore formed on a thin film with a thickness of more than ten nanometers, and its side shape is hourglass-shaped or cylindrical; the other is formed on a single-layer graphene 、MoS 2 Fabricate nanopores in two-dimensional materials. The processing of the above nanopores generally involves the following micro-manufacturin...

AI Technical Summary

Problems solved by technology

The currently prepared nanopores generally have several problems in the detection of antigens and antibodies: 1. The ion current blocking signal when the antigen molecule passes through the nanochannel and the collision signal caused by the movement of the antigen molecule to the vicinity of the pore cannot be effectively distinguished

2. It is impossible to directly judge whether the antigen molecule passes through the hole from the change of the ion current signal

3. Antigen detection and antigen / antibody conjugate detection cannot be performed continuously, the experimental operation is complicated, and it is difficult to guarantee the accuracy of the experiment

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