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Liquid fermentation culture medium for cordyceps sinensis

A technology of liquid fermentation and culture medium, applied in fungi, microorganism-based methods, microorganisms, etc., can solve the problems of cumbersome process flow, improvement, limited crushing effect, etc.

Active Publication Date: 2016-08-24
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cordyceps sinensis's existing Cordyceps sinensis fermentation technology plan "A bionic cultivation method of Cordyceps sinensis" (patent number: ZL200510104832.9) mentioned that the use of mechanical external force crushing can increase the growth point of Cordyceps sinensis, thereby improving the efficiency of fermentation, However, the crushing scheme of mechanical external force often damages a large number of cells, and the crushing effect is limited. It is necessary to add crushing equipment in the fermentation device, thereby increasing the potential source of pollution, so the process is cumbersome; another technical scheme "solid fermentation method of Cordyceps sinensis" (Patent No.: ZL200910197957.9) mentions that the seed liquid of liquid culture has the characteristics of more growth points, but does not mention how to promote the improvement of the number of growth points in the fermentation medium

Method used

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  • Liquid fermentation culture medium for cordyceps sinensis
  • Liquid fermentation culture medium for cordyceps sinensis

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Experimental program
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Effect test

Embodiment 1

[0014] Negative control liquid fermentation medium is made up of 10g / L trehalose, 5g / L glucose, 15g / L peptone, 5g / L yeast extract, 0.5g / L magnesium sulfate and 1g / L potassium dihydrogen phosphate; the liquid of the present invention Fermentation medium is added 18mmol / L phosphorylcholine on the basis of above-mentioned formula. Inoculate Cordyceps sinensis strain (Hermelia sinensis) in two groups of culture medium respectively, after 120rpm shaking table 15 ℃ cultivated for 20 days, take out the fermentation broth for microscopic examination, count by cell counting method, microscopic examination shows blastospores in the liquid culture fluid (or claim mycelia section), the blastospore concentration in the liquid fermentation medium of the present invention is 1.2-1.5 * 10 3 cells / mL, while only 0.3-0.5×10 in the negative control medium 3 individual / mL.

Embodiment 2

[0016] Negative control liquid fermentation medium is made up of 20g / L trehalose, 0.5g / L glucose, 5g / L peptone, 10g / L yeast extract, 0.5g / L magnesium sulfate and 1g / L potassium dihydrogen phosphate; The liquid fermentation medium was added 25mmol / L phosphoethanolamine on the basis of the above formula. Inoculate Cordyceps sinensis strain (Hermelia sinensis) in two groups of culture medium respectively, after 15 days of culturing on a 120rpm shaker at 18°C, take out the fermentation broth for microscopic examination, and use the cell counting method for statistics. Microscopic examination shows blastospores in the liquid culture fluid (or claim mycelia section), the blastospore concentration in the liquid fermentation medium of the present invention is 1.3-1.4 * 10 3 / mL, while only 0.2~0.4×10 in the negative control medium 3 individual / mL.

Embodiment 3

[0018] The negative control liquid fermentation medium is made up of 25g / L trehalose, 10g / L glucose, 35g / L peptone, 10g / L yeast extract, 0.5g / L magnesium sulfate and 1g / L potassium dihydrogen phosphate; the liquid of the present invention The fermentation medium was supplemented with 10mmol / L of phosphorylcholine and 35mmol / L of phosphoethanolamine on the basis of the above formula. Inoculate Cordyceps sinensis strain (Hermelia sinensis) in two groups of culture medium respectively, after 120rpm shaker culture at 16°C for 30 days, take out the fermentation broth for microscopic examination, and count by cell counting method, microscopic examination shows blastospores in the liquid culture fluid (or claim mycelia section), the blastospore concentration in the liquid fermentation medium of the present invention of statistical result is 2.8-3.0 * 10 3 cells / mL, while only 0.7-0.8×10 in the negative control medium 3 individual / mL.

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Abstract

The invention relates to a liquid fermentation culture medium for cordyceps sinensis. In additions to a carbon source, a nitrogen source, metal salts and the like in a common culture medium, the culture medium is further added with phosphorylcholine, phosphorylethanolamine, cholesterol, fatty acid and the like as a growth promoter, so that the cordyceps sinensis can be effectively promoted to form blastospore or hyphal bodies. Bacterial suspension obtained by culture medium fermentation is rich in blastospore or hyphal body growth point, and can be continuously fermented or used as a seed solution for subsequent liquid or solid fermentation, and therefore, the liquid fermentation culture medium is beneficial for improving fermentation production efficiency of the cordyceps sinensis.

Description

technical field [0001] The invention relates to a culture medium of microorganisms, in particular to a liquid fermentation medium of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is a characteristic precious medicinal material in the Qinghai-Tibet Plateau of my country. It is distributed in high-altitude areas of the Qinghai-Tibet Plateau. Its growth range is limited and its output is extremely limited. It has important medicinal and economic values. Its nature and taste are sweet and flat, and it has the effects of protecting the lungs and kidneys, stopping bleeding and resolving phlegm, and relieving cough. Modern medical research has found that Cordyceps sinensis also has unique effects such as inhibiting tumors, anti-cancer and significantly improving human immune function. [0003] As a kind of entomoascus fungus, the life cycle of Cordyceps sinensis includes two parts: sexual stage and asexual stage. Both morphological...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 钟欣刘昕张古忍沈冬秀阚绪甜李少松王海贞古励
Owner SUN YAT SEN UNIV
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