Method for cultivating antrodia camphorata fruiting bodies by use of applewood and culture medium

A medium and apple wood technology, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of low content of active products, slow growth, long production cycle, etc., shorten the cultivation time, obtain raw materials easily, and prevent burning Effect

Active Publication Date: 2016-08-31
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial culture technology of Antrodia cinnamomea has made great progress, in general, the development and application of its culture technology and products are still in the primary and middle stages
[0006] The solid culture of the prior art is to inoculate the Antrodia camphorata strains in solid agar medium or space bag for cultivation, and the cultivation time is usually several months, and it is difficult to cultivate into fruiting bodies
[0007] The liquid culture of the prior art can obtain a large amount of mycelia and fermentation liquid in a relatively short period of time, can greatly increase the yield, and can make up for the shortcoming of slow growth of Antrodia camphorata, but the content of its active product is relatively low
[0008] The basswood cultivation method in the prior art is to inoculate Antrodia camphorata on the basswood of Antrodia camphorata, the obtained Antrodia camphorata components are similar to the types of physiologically active substances contained in wild Antrodia camphorata, and can obtain higher quality Antrodia camphorata fruiting bodies, but Because its growth is extremely slow, the production cycle is long, and the camphor tree is a first-class protected tree species in Taiwan, and resources are limited, which greatly limit the production of Antrodia camphorata

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: based on solid bacterial classification

[0030] Strain: Antrodia camphorata strain, purchased from Guangdong Microbial Strain Collection Center, stored on PDA slant medium.

[0031] Preparation of carrot medium: raw materials include: carrot 20% (W / V), glucose 2% (W / V), agar 1.5% (W / V), remaining water, pH natural; carrots cut into 1cm 3 Size, boiled in water for 20 minutes, filtered to get the filtrate for use.

[0032] Preparation of solid seeds: Divide the prepared carrot medium into 500ml conical flasks, 250ml per bottle, seal with a special sealing film for edible fungi, and sterilize at 121°C for 30min. Put the sterilized culture dish, inoculation needle, melted culture medium in the Erlenmeyer flask, and the alcohol lamp into the ultra-clean workbench, then sterilize with the ultraviolet lamp for 30 minutes, turn on the ventilation, disinfect the hands with alcohol cotton balls, light the alcohol lamp, and start Aseptically operate, pour the pla...

Embodiment 2

[0037] Embodiment 2: based on liquid strain

[0038] Strain: Antrodia camphorata strain, purchased from Guangdong Microbial Strain Collection Center, stored on PDA slant medium.

[0039] Preparation of carrot medium: raw materials include: carrot 20% (W / V), glucose 2% (W / V), agar 1.5% (W / V), remaining water, pH natural; carrots cut into 1cm 3 Size, boiled in water for 20 minutes, filtered to get the filtrate for use.

[0040] Preparation of liquid seed medium: wort with 5% solids (W / V), natural pH.

[0041] Activation of strains: use carrot medium to activate the preserved strains, and place them in a constant temperature incubator at 28°C for 15 days; put the cryopreserved Antrodia camphorata strains into carrot medium under aseptic conditions, and culture them in the dark at 28°C 15 days.

[0042] Preparation of liquid Antrodia camphorata seeds: Divide the prepared liquid seed medium into 500ml conical flasks, 150ml per bottle, seal with a special parafilm for edible fun...

Embodiment 3

[0047] Embodiment 3: Based on the liquid strain

[0048] Strain: Antrodia camphorata strain, purchased from Guangdong Microbial Strain Collection Center, stored on PDA slant medium.

[0049] Preparation of carrot medium: raw materials include: carrot 20% (W / V), glucose 2% (W / V), agar 1.5% (W / V), remaining water, pH natural; carrots cut into 1cm 3 Size, boiled in water for 20 minutes, filtered to get the filtrate for use.

[0050] Preparation of liquid seed medium: wort with 5% solids (W / V), natural pH.

[0051] Activation of strains: use carrot medium to activate the preserved strains, and place them in a constant temperature incubator at 28°C for 15 days; put the cryopreserved Antrodia camphorata strains into carrot medium under aseptic conditions, and culture them in the dark at 28°C 15 days.

[0052] Preparation of liquid seeds: Divide the prepared liquid seed culture medium into 500ml conical flasks, 150ml per bottle, seal with a special sealing film for edible fungi, ...

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PUM

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Abstract

The invention discloses a method for cultivating antrodia camphorata fruiting bodies by the use of applewood. The method comprises the following steps: culturing antrodia camphorata in a liquid seed medium or a solid seed medium under the antrodia camphorata growth conditions so as to obtain liquid or solid antrodia camphorata seeds; carrying out soak cleaning by the use of applewood, putting into a polypropylene fungus sack, sealing and fastening and sterilizing; opening the fungus sack filled with the applewood in an aseptic environment, inoculating the solid or liquid antrodia camphorata seeds, sealing and fastening; carrying out dark culture on the inoculated fungus sack to complete nutritional growth management; and releasing sack for culturing until antrodia camphorata fruiting bodies grow to 2cm and above, and picking to complete the reproductive growth management. Raw materials of a carrot medium comprise a filtrate obtained after carrots are boiled with boiling water and then filtered, glucose, agar and rest of water. Raw materials of the liquid seed medium comprise wort containing 5% (W/V) of solids. A culture medium is the applewood. The method is simple to operate, culture period is relatively short. Sources of raw materials are wide. Cost is low.

Description

Technical field: [0001] The invention discloses a method for cultivating fruiting bodies of Antrodia camphorata by using apple wood and a culture medium thereof, which relates to a method for cultivating Antrodia camphorata fruiting bodies by using apple wood as a raw material under artificial cultivation conditions, and belongs to the technical field of cultivation of edible and medicinal fungi. Background technique: [0002] Antrodia Cinnamomea ( Antrodia camphorata ), also known as camphor mushroom, camphor mushroom, red Antrodia camphorata, belongs to Basidiomycotina ( Basidiomycotina ), Laminaria ( Hymenomycetes ), Polyporaceae ( Polyporaceae ), Lepiporia ( Antrodia ), is a rare medicinal fungus native to Taiwan. Antrodia cinnamomea is native to the 100-year-old camphor tree ( Cinnamomea kanehirai ) The inner wall of the decayed heartwood of the trunk is an authentic medicinal material in Taiwan, known as "Taiwan Treasure" and "Ruby in Taiwan Forest". Due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01G1/04C12N1/14
CPCA01G18/00C05F11/00C05G3/00C12N1/14
Inventor 程显好冯路瑶刘静孙磊郝宝健
Owner LUDONG UNIVERSITY
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