A method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application
A technology for tuberculous meningitis and viral meningitis, which can be applied in the biological field and can solve the problem of limited database of matrix effect compounds
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Embodiment 1
[0051] Example 1. A method for distinguishing tuberculous meningitis from viral meningitis based on cerebrospinal fluid HNMR technology
[0052] 1. Collection and processing of cerebrospinal fluid samples
[0053] (1) The cerebrospinal fluid of 20 patients with tuberculous meningitis (TBM) and 19 patients with viral meningitis (VM) was collected routinely (the cerebrospinal fluid was obtained from Beijing Chest Hospital Affiliated to Capital Medical University, and all patients were confirmed by clinical diagnosis. and the patient was informed), centrifuged at 2000 g for 10 min at 4°C, filtered through a 0.22 μm filter membrane, and stored at -80°C.
[0054] (2) Before the experiment, the cerebrospinal fluid sample was thawed at room temperature, and centrifuged at 13000 g for 10 min at 4°C to obtain the centrifuged cerebrospinal fluid.
[0055] (3) The centrifuged cerebrospinal fluid, 50 μl TSP heavy aqueous solution (TSP concentration is 1 mg / ml) and 200 μl D 2 O was mixed...
Embodiment 2
[0070] Example 2, the verification of the method for distinguishing the patients to be tested as tuberculous meningitis patients and viral meningitis patients
[0071] The cerebrospinal fluid of one patient with tuberculous meningitis (TBM) and one patient with viral meningitis (VM) was collected routinely (the cerebrospinal fluid was obtained from Beijing Chest Hospital Affiliated to Capital Medical University, and all patients were clinically diagnosed and informed ), according to the method for distinguishing tuberculous meningitis and viral meningitis based on cerebrospinal fluid HNMR technology in embodiment 1, the cerebrospinal fluid of above-mentioned tuberculous meningitis (TBM) patient and viral meningitis (VM) patient is carried out nuclear magnetism respectively The relative expression levels of the differential metabolites represented by the chemical shifts of 3.50ppm, 3.76ppm, 3.05ppm and 2.79ppm were obtained respectively.
[0072] The test results show that: the...
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