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A method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application

A technology for tuberculous meningitis and viral meningitis, which can be applied in the biological field and can solve the problem of limited database of matrix effect compounds

Active Publication Date: 2018-11-13
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The former has higher sensitivity, but the disadvantages are that the sample needs to be pre-treated and ionized, has matrix effects, and the database for identifying compounds is limited, etc.

Method used

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  • A method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application
  • A method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application
  • A method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1. A method for distinguishing tuberculous meningitis from viral meningitis based on cerebrospinal fluid HNMR technology

[0052] 1. Collection and processing of cerebrospinal fluid samples

[0053] (1) The cerebrospinal fluid of 20 patients with tuberculous meningitis (TBM) and 19 patients with viral meningitis (VM) was collected routinely (the cerebrospinal fluid was obtained from Beijing Chest Hospital Affiliated to Capital Medical University, and all patients were confirmed by clinical diagnosis. and the patient was informed), centrifuged at 2000 g for 10 min at 4°C, filtered through a 0.22 μm filter membrane, and stored at -80°C.

[0054] (2) Before the experiment, the cerebrospinal fluid sample was thawed at room temperature, and centrifuged at 13000 g for 10 min at 4°C to obtain the centrifuged cerebrospinal fluid.

[0055] (3) The centrifuged cerebrospinal fluid, 50 μl TSP heavy aqueous solution (TSP concentration is 1 mg / ml) and 200 μl D 2 O was mixed...

Embodiment 2

[0070] Example 2, the verification of the method for distinguishing the patients to be tested as tuberculous meningitis patients and viral meningitis patients

[0071] The cerebrospinal fluid of one patient with tuberculous meningitis (TBM) and one patient with viral meningitis (VM) was collected routinely (the cerebrospinal fluid was obtained from Beijing Chest Hospital Affiliated to Capital Medical University, and all patients were clinically diagnosed and informed ), according to the method for distinguishing tuberculous meningitis and viral meningitis based on cerebrospinal fluid HNMR technology in embodiment 1, the cerebrospinal fluid of above-mentioned tuberculous meningitis (TBM) patient and viral meningitis (VM) patient is carried out nuclear magnetism respectively The relative expression levels of the differential metabolites represented by the chemical shifts of 3.50ppm, 3.76ppm, 3.05ppm and 2.79ppm were obtained respectively.

[0072] The test results show that: the...

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Abstract

The invention discloses a method for distinguishing tubercular meningitis and virus meningitis based on a nuclear magnetic resonance technology and application thereof. According to the method, nuclear magnetic resonance detection is conducted on the cerebrospinal fluid of a patient to be detected to obtain relative expression quantities of difference metabolites represented by chemical shifts of 3.50 ppm, 3.76 ppm, 3.05 ppm, and 2.79 ppm respectively, and then whether the patient suffers from the tubercular meningitis or the virus meningitis is distinguished according to the relative expression quantities of the difference metabolites. Compared with the prior art, the method has the advantages of being simple in sample treatment, free of sample damage, small in sample quantity, good in repeatability, low in cost and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for distinguishing tuberculous meningitis from viral meningitis based on nuclear magnetic resonance technology and its application, in particular to a method for distinguishing tuberculous meningitis from viruses based on cerebrospinal fluid nuclear magnetic resonance metabolomics technology Methods and applications for meningitis. Background technique [0002] Tuberculous meningitis (TBM) is a kind of nervous system tuberculosis caused by Mycobacterium tuberculosis infection. TBM accounts for about 1% of active tuberculosis, but it is the most severe type of tuberculosis, with a mortality rate of up to 60%, and survivors are often accompanied by severe disability and neurological sequelae. [0003] Early diagnosis of TBM and timely anti-tuberculosis treatment are the key to improving survival rate and reducing complications, but there is still a lack of effectiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/055
CPCA61B5/055
Inventor 李自慧张宗德刘菲杜博平曹廷明郑晓静刘忠泉陈曦潘丽萍吕翎娜杜凤娇黄银霞
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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