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A lung cancer screening kit
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A reagent and technology for lung cancer, applied in the field of lung cancer screening kits, can solve the problems of missed cure opportunity and low survival rate, and achieve the effect of good clinical application prospects.
Active Publication Date: 2018-09-18
WEST CHINA HOSPITAL SICHUAN UNIV
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[0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best time to cure, the five-year survival rate is low
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Embodiment 1
[0020] Example 1 Relationship between LCK expression level and lung cancer
[0021] 1. Experimental method
[0022] 1. Clinical data
[0023] 30 cases of paraffin sections of lung cancer patients and 20 cases of remote controls (refers to the paraffin sections of distal normal lung tissue of lung cancer patients, 5 cm away from the cancer tissue) were selected. The basic information is shown in Table 1.
[0024] Table 1 Basic clinical information
[0025]
[0026] * Note: Others include large cell carcinoma, adenosquamous carcinoma, and small cell carcinoma.
[0027] 2. Detection of LCK expression level
[0028] Immunohistochemistry (IHC) using LCK as an index was performed on paraffin sections of lung cancer patients and control paraffin sections of distant tissues.
[0029] LCK antibody: purchased from American Affinity Company, Cat. No. AF6101, to detect total LCK protein;
[0059] Embodiment 2 The composition of the kit for detecting the expression level of LCK of the present invention and its application method
[0060] 1. The composition of the immunohistochemical detection kit
[0061] Detection kit (50 copies):
[0062] components
volume
LCK antibody
30μl
EnVision TM HRP secondary antibody
100μl
Antibody Diluent
10ml
DAB developer
10ml
Antigen Retrieval Solution
500ml
Wash Buffer *
500ml
immunohistochemistry pen
1 stick
[0063] Note: This kit is calculated as 100 μl of various experimental reagents per slice, and the specific dosage can be appropriately increased or decreased according to the size of the tissue.
[0064] 2. How to use the kit
[0065] The lung tissue of the sample to be tested was prepared as a paraffin section as a test specimen, and the expression level of LCK was detected according to the following method:
[0066] (1) Blocki...
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Abstract
The invention discloses a lung cancer screening kit. The lung cancer screening kit comprises an optional reagent which is used for testing expression level of LCK (Lymphocyte Cell-specific protein-tyrosine Kinase). The invention further discloses use of the reagent for testing the expression level of the LCK in preparation of reagents for lung cancer screening. According to the kit disclosed by the invention, through testing the expression level of the LCK, the risk of suffering from a lung cancer of a testee can be judged; the kit can be applied to the clinical auxiliary diagnosis of the lung cancer so as to provide an effective basis for taking related therapy measures or decisions by a patient; the clinical application prospect is good.
Description
technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...
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