Application of theanine in preparation of products for preventing and treating cancer diseases

A technology of theanine and inflammation, applied in the medical field, can solve problems such as toxic side effects and limited prevention and treatment

Inactive Publication Date: 2016-09-28
张国营
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the toxic and side effects of clinical chemotherapy and radiotherapy, the effective prevention and treatment of it is limited

Method used

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  • Application of theanine in preparation of products for preventing and treating cancer diseases
  • Application of theanine in preparation of products for preventing and treating cancer diseases
  • Application of theanine in preparation of products for preventing and treating cancer diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Example 1: Theanine and its mixture with 6-fluoro-4-hydroxycoumarin inhibit the growth of cancer cells in vitro Inhibition experiment:

[0021] The MTT method was used to test the inhibitory effect of theanine and its mixture with 6-fluoro-4-hydroxycoumarin on the growth of cancer cells in vitro. Proceed as follows:

[0022] 1. Main reagents, cell lines and instruments:

[0023] Human pancreatic cancer PANC-1 and human cervical cancer Hela cell lines and instruments were described above.

[0024] RPMI1640 and DMEM culture medium: Hyclone Company; inactivated fetal bovine serum: Hyclone Company;

[0025] Trypsin (trypsin): Amersco Company; 0.4% trypan blue: Sigma Company; Tetramethylazozolium salt (MTT): Sigma Company;

[0026] 2. Experimental steps:

[0027] (1) Digest the cancer cells in logarithmic growth phase with 0.25% trypsin to make a single cell suspension and adjust the cell concentration to 5×10 4 / mL, inoculated in 96-well culture plate with 100 μl p...

Embodiment 2

[0041](2) Example 2: Theanine and its mixture with coumarin inhibit cancer cell invasion inhibition experiment in vitro:

[0042] 1. Main reagents, cell lines and instruments:

[0043] Human pancreatic cancer PANC-1 and human cervical cancer Hela cell lines and instruments were described above.

[0044] RPMI1640 and DMEM culture medium: Hyclone Company; inactivated fetal bovine serum: Hyclone Company;

[0045] Trypsin (trypsin): Amersco; propidium iodide (PI): Sigma, USA.

[0046] Matrigel, Fibronectin: BD Company of the United States; Transwell Chamber: Costar Company of the United States; cell culture plastic plate (24 wells): BD Company.

[0047] 2. Experimental steps:

[0048] (1) Place the Transwell chamber in a 24-well plate, spread 100 μl / well of Fibronectin and Matrigel evenly on the polycarbonate membrane at the bottom of the chamber, incubate at 37°C for 2 hours, and then air-dry in an ultra-clean workbench;

[0049] (2) Cells were collected, and the upper chambe...

Embodiment 3

[0059] (3) Example 3: The experiment of theanine inhibiting the growth of human cancer xenografts in nude mice

[0060] 1. Experimental animals, cell lines, main reagents and instruments:

[0061] SPF grade BALB / c nude mice, 4-5 weeks old, 18-22g, male, purchased from Beijing Huafukang Experimental Animal Center, animal license number SCXK (Beijing) 2009-0004, bred in SPF grade animal laboratory ; IVC was independently sent back to the air cage, Suzhou Suhang Technology Equipment Co., Ltd.; human pancreatic cancer PANC-1 and human cervical cancer Hela cell lines and other instruments were as described above.

[0062] RPMI1640 and DMEM culture medium: Hyclone Company; inactivated fetal bovine serum: Hyclone Company, stored at -20°C; trypsin: Amersco Company; 0.4% trypan blue: Sigma Company;

[0063] 2. Experimental steps:

[0064] (1) Cell culture and establishment of transplanted tumor animal models: Human pancreatic cancer PANC-1 and human cervical cancer Hela cancer cell l...

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Abstract

The invention relates to the technical field of medical treatment. Theanine is a characteristic amino acid of tea, can be used for inhibiting cell growth and invasion of human pancreatic cancer, cervical cancer, gastric cancer, breast cancer and other cancers as well as remarkably inhibiting growth of tumors in a body, and has an inhibition effect superior to anti-cancer medicines such as cyclophosphamide. The action mechanism relates to down-regulation of receptors closely related to inhibition of tumor growth, invasion and transfer, signal transduction regulated protein, kinase level and combination of nuclear factors and DNA, as well as upper regulation of factors such as cancer-inhibiting protein and cell cycle inhibition protein; and the theanine can be used for directly inhibiting the activity of histone deacetylase and histone methyltransferase EZH2 and combination of inflammatory factor NF-kappa B and DNA. Furthermore, the theanine can be synergized with 6-fluoro-4-hydroxycoumarin (X) to improve inhibition on tumor cell growth and invasion by times, and has the activity superior to that of cyclophosphamide and the like. The theanine and synthesis with X can be applied to prevention and treatment of tumors, inflammations, cardiovascular diseases, immunodeficiency and other diseases.

Description

[0001] This application is a divisional application of "Application of Theanine, etc. in the Preparation of Products Preventing and Treating Cancer and Other Diseases". The filing date of the original application is April 28, 2012, and the application number is 201210138933.8. technical field [0002] The invention relates to the field of medical technology, in particular to the application of theanine and its mixture with 6-fluoro-4-hydroxycoumarin in the preparation of disease inhibitors for the prevention and treatment of tumors, inflammation, cardiovascular and cerebrovascular diseases, and immunodeficiency. Background technique [0003] The abnormal growth, invasion and metastasis of malignant tumors such as human pancreatic cancer, cervical cancer, gastric cancer and estrogen receptor-negative human breast cancer are the important causes of death of tens of thousands of patients every year, and the incidence is also maintained at a certain level. It has become a major d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A61P9/00A61P29/00A61P35/00A61P37/02
CPCA61K31/198A61K31/37A61K2300/00
Inventor 张国营
Owner 张国营
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