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Separation method and application of high altitude anoxia-induced fatigue-resistant active ingredient in gardenia jasminoides ellis

A technology of active ingredients and separation methods, applied in the field of separation of anti-altitude anoxic fatigue active ingredients in gardenia, can solve problems such as accumulation of blood lactic acid and other metabolites, metabolic acid-base disorders, and decreased labor capacity

Active Publication Date: 2016-09-28
中国人民解放军联勤保障部队第九四O医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, plateau hypoxia has the most obvious impact on the body. After engaging in certain physical or mental work in a plateau hypoxic environment, it is very easy to cause blood lactic acid and other metabolites to accumulate, and cause metabolic acid-base disorders in the body, resulting in muscle tension. The decline leads to the occurrence of fatigue, which shows a general decline in labor capacity, and severe cases lose the ability to work

Method used

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  • Separation method and application of high altitude anoxia-induced fatigue-resistant active ingredient in gardenia jasminoides ellis
  • Separation method and application of high altitude anoxia-induced fatigue-resistant active ingredient in gardenia jasminoides ellis
  • Separation method and application of high altitude anoxia-induced fatigue-resistant active ingredient in gardenia jasminoides ellis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Example 1 The separation method of anti-altitude hypoxic fatigue active ingredients in Gardenia, comprising the following steps:

[0173] (1) Dried and crushed gardenia fruits to 20 meshes, decocting and extracting them with distilled water, filtering, concentrating under reduced pressure, and spray-drying to obtain gardenia powder.

[0174] Among them: decoction extraction conditions refer to the mass ratio of solid to liquid (g / g) is 1:8, the temperature is 90°C, the number of times is 2 times, and each time is 1 hour.

[0175] The strip-dried items concentrated under reduced pressure refer to a temperature of 50°C.

[0176] The conditions of spray drying refer to a temperature of 120°C and an air intake of 20 m per unit time. 3 , spray pressure 0.1MPa.

[0177] (2) Prepare gardenia powder with distilled water at 25°C to make a 20 mg / mL aqueous solution.

[0178] (3) Inject the aqueous solution obtained in step (2) into the treated 14-mesh polyamide column at a flo...

Embodiment 2

[0188] Example 2 The separation method of anti-altitude hypoxic fatigue active ingredients in Gardenia, comprising the following steps:

[0189] (1) Dried and crushed gardenia fruit to 30 meshes, decocted and extracted with distilled water, filtered, concentrated under reduced pressure, and spray-dried to obtain gardenia powder.

[0190] Among them: the decoction extraction conditions refer to the solid-liquid mass ratio (g / g) of 1:10, the temperature of 100°C, the number of times is 3 times, and the time of each time is 1 hour.

[0191] The condition of concentration under reduced pressure refers to a temperature of 80°C.

[0192] The conditions of spray drying refer to a temperature of 140°C and an air volume of 30 m per unit time. 3 , spray pressure 0.4MPa.

[0193] (2) Prepare gardenia powder with distilled water at 25°C to make a 30 mg / mL aqueous solution.

[0194] (3) Inject the aqueous solution obtained in step (2) into the treated 30-mesh polyamide column at a flow ...

Embodiment 3

[0204] Example 3 The separation method of the anti-altitude hypoxic fatigue active ingredient in Gardenia jasminoides, comprising the following steps:

[0205] (1) Dried and crushed gardenia fruits to 40 meshes were decocted and extracted with distilled water, filtered, concentrated under reduced pressure, and spray-dried to obtain gardenia powder.

[0206] Among them: the decoction extraction conditions refer to the solid-liquid mass ratio (g / g) of 1:9, the temperature of 95°C, the number of times is 2 times, and each time is 1 hour.

[0207] The condition of concentration under reduced pressure refers to a temperature of 60°C.

[0208] The conditions of spray drying refer to a temperature of 130°C and an air volume of 25 m per unit time. 3 , Spray pressure 0.2MPa.

[0209] (2) Prepare gardenia powder with distilled water at 25°C to make a 40 mg / mL aqueous solution.

[0210] (3) Inject the aqueous solution obtained in step (2) into the treated 20-mesh polyamide column at a...

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Abstract

The invention relates to a separation method for a high altitude anoxia-induced fatigue-resistant active ingredient in gardenia jasminoides ellis. The method comprises the following steps: (1) performing aqueous extraction on gardenia jasminoides ellis fruits, and performing filtration, concentration and drying to obtain gardenia jasminoides ellis powder; (2) preparing an aqueous solution from the gardenia jasminoides ellis powder; (3) injecting the aqueous solution obtained in step (2) into a treated polyamide column, and performing flushing with distilled water to obtain polyamide column sample effluent and aqueous eluent respectively; (4) mixing and injecting the polyamide column sample effluent and the aqueous eluent into a treatment macroporous resin column, and performing elution, reduced pressure concentration and freeze drying to obtain a geniposide product; (5) flushing the polyamide column to remove impurities with a 10 to 30 percent ethanol solution, flushing the polyamide column with a 30 to 90 percent ethanol solution to obtain 30 to 90 percent ethanol eluent, and performing reduced pressure concentration and freeze drying on the eluent to obtain a gardenia jasminoides ellis yellow pigment product. In addition, the invention also discloses the application of the gardenia jasminoides ellis yellow pigment product. The separation method is simple, easy and convenient to operate, and industrial production can be implemented.

Description

technical field [0001] The invention relates to a process for enriching effective components of Chinese herbal medicines, in particular to a method for separating active components from Gardenia jasminoides for anti-plateau anoxic fatigue and application thereof. Background technique [0002] After the plain people entered the plateau, due to environmental factors such as thin air, low atmospheric pressure, and low partial pressure of oxygen in the plateau area, the physical strength and military operation ability of the body were directly affected. Among them, plateau hypoxia has the most obvious impact on the body. After engaging in certain physical or mental work in a plateau hypoxic environment, it is very easy to cause blood lactic acid and other metabolites to accumulate, and cause metabolic acid-base disorders in the body, resulting in muscle tension. The decline leads to the occurrence of fatigue, which shows a general decline in labor capacity, and severe cases lose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/744A61P39/00A61P39/06C09B61/00
CPCA61K36/744A61K2236/331A61K2236/39A61K2236/51A61K2236/55C09B61/00
Inventor 李茂星王先敏毛婷曹馨元马幸福
Owner 中国人民解放军联勤保障部队第九四O医院
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