The invention discloses a biological compound preparation capable of improving the high altitude anoxia tolerance. The biological compound preparation is prepared from the following raw materials in parts by weight: 2-10 parts of folium ginkgo extract, 5-15 parts of guarana extract, 10-16 parts of semen cassiae extract, 2-8 parts of gynostemma pentaphylla extract, 20-40 parts ofherba rhodiolae, 15-30 parts of caulis sargentodoxae, 10-18 parts of semen armeniacae amarae, 5-15 parts of radix patriniae, 5-15 parts of ramulus et folium murrayae, 10-30 parts of radix astragali, 15-30 parts of purslane speedwell herb, 7-16 parts of radix codonopsis, 2-10 parts of fructus tribuli, 5-10 parts of all-grass of neottopteris nidus, 5-10 parts of spirulina powder, 10-20 parts of cordyceps, 10-20 parts of radix ginseng, 20-40 parts of tea leaves, 5-10 parts of folium ginkgo, 2-7 parts of Chinese wolfberry fruits, 2-8 parts of fructus ziziphi jujubae, 2-10 parts of fructus wild jujubae, 2-7 parts of flos chrysanthemi, 4-10 parts of longans, 4-10 parts of herba epimedii, 2-7 parts of radix ophiopogonis, 1-10 parts of cortex cinnamomi, and 5-12 parts of soybean oligosaccharides. The biological compound preparation can improve the high altitude anoxia tolerance, no chemicals are added, and therefore, the biological compound preparation causes no harm to the human body and is small in toxic and side effect after being taken for a long term.