Methods for establishing an animal model of acute high altitude hypoxia and anxiety stress response

A stress response and animal model technology, applied in animal husbandry and other fields, can solve problems such as difficult application of acute plateau hypoxia and anxiety stress response, failure to achieve intervention effect, intervention mechanism research, etc.

Active Publication Date: 2020-02-14
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the prevention and treatment of altitude stress include traditional Chinese medicine and western medicine and physical intervention, but due to the lack of animal models that are close to the induction factors of altitude acute stress and have similar pathological reactions, the existing normal pressure airtight hypoxia, drugs, etc. Animal models of hemorrhagic hypoxia or anxiety cannot achieve the purpose of accurately evaluating the effect of intervention and conducting in-depth research on the mechanism of intervention
[0005] Chinese patent ZL.2013102565702 discloses a method for establishing an animal model of Alzheimer's disease, which is difficult to apply to acute plateau hypoxia and anxiety stress response

Method used

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  • Methods for establishing an animal model of acute high altitude hypoxia and anxiety stress response
  • Methods for establishing an animal model of acute high altitude hypoxia and anxiety stress response
  • Methods for establishing an animal model of acute high altitude hypoxia and anxiety stress response

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1 Preparation of Animal Models of Acute Plateau Hypoxia and Anxiety Stress Response

[0037] 1. Experimental animal feeding and grouping

[0038] 80 male Kunming mice, weighing 20±2g. Adaptive feeding for 1 week, free access to food and water, providing artificial biorhythm conditions in which 16 hours of light and 8 hours of darkness alternate, and the room temperature of the feeding environment is 25±2°C.

[0039] After one week of adaptive feeding, the animals were randomly divided into plain 3d group (3P), plain 7d group (7P), plateau 3d group (3G), and plateau 7d group (7G), with 20 animals in each group. Altitude, 22 ± 2 ℃ cold water swimming 30min × 2 times / day × 2 days after adaptive training, the 3G group and the 7G group were kept in a 5500m cabin, and the temperature control and lighting control conditions were consistent with the plain group.

[0040] The animals in the 3G group and 7G group were respectively given 3500m plateau combined with 22±...

Embodiment 2

[0084] Example 2 Drug Intervention of Animal Models of Acute Plateau Hypoxia and Anxiety Stress Response

[0085] 1. Drug extraction

[0086] Tibetan medicine Rhodiola, Gastrodia elata, Rehmannia glutinosa, and Hypericum perforatum are all purchased from Shanghai Leiyunshang Traditional Chinese Medicine Sales Department. Rhodiola rosea, Hypericum perforatum, Gastrodia elata, and Rehmannia glutinosa were washed, dried, crushed, soaked in edible ethanol for 8-12 hours, extracted at a constant temperature of 80°C-90°C and protected from light for 40 minutes, and extracted three times continuously. Add 0.01-0.5% chitosan colloidal solution at 40°C, and stir at 50 r / min for 5 min. Let it stand for more than 12 hours, filter, concentrate under negative pressure, add distilled water to adjust the compound drug extract to 1.0g / ml crude drug content, and store it in a refrigerator at 4°C for later use, hereinafter referred to as compound drug or FJJOL). The above compound drug extrac...

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Abstract

The invention provides a method for establishing an acute high altitude anoxia and anxiety stress response animal model. The method comprises the following steps of (1) raising mice at conventional elevation environment, and performing cold water forced swimming adaptability training; (2) and then enabling the mice obtained in the step (1) to be exposed to high-elevation environmental conditions for raising, and performing high-elevation environment composite cold water forced swimming so as to obtain the acute high altitude anoxia and anxiety stress response animal model. The acute high altitude anoxia and anxiety stress response animal model obtained by the method disclosed by the invention has cerebral anoxia pathology characteristics that an aerobic metabolism way of sugar in brain tissues is blocked, so that the supply of ATP energy sources is obviously reduced, and lactic acid is accumulated, and behavioral expression of anorexia and enhanced anxiety behavior, and the induced stimulating factors and clinical pathology behavioral expression of the model both approach the actual situations of acute altitude stress response.

Description

technical field [0001] The invention relates to the fields of hypobaric oxygen medicine and biology, in particular, the invention relates to a method for establishing an animal model of acute plateau hypoxia and anxiety stress response. Background technique [0002] Acute plateau hypoxia and anxiety stress response is a physiological or pathological condition caused by the body's acute exposure to the plateau environment and the lack of endogenous oxygen caused by the low-pressure hypoxic environment, but at the same time must bear a certain workload The symptoms of adverse reactions mainly include dyspnea, dizziness, nausea, anxiety and fatigue. [0003] The hypobaric chamber is a kind of large-scale experimental equipment for special purposes such as research, training and treatment in the fields of aviation medicine, sports medicine and clinical medicine. [0004] With the opening of the Qinghai-Tibet Railway, the number of people entering Tibet has expanded from special...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2267/03
Inventor 朱伟沈俊张慧刘秋红
Owner NAVY MEDICINE RES INST OF PLA
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