43results about How to "Improves resistance to oxidative stress" patented technology

The invention discloses lactobacillus reuteri capable of intervening in metabolic syndrome and application, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1145 screened by the invention can significantly improve the increase of serum total cholesterol content of metabolic syndrome mice caused by high fat diet. The weight rise of metabolic syndrome individuals is relieved, and the daily diet intake and the energy efficiency are remarkably reduced. Fasting blood glucose and insulin resistance index levels of metabolic syndrome individuals are significantly improved. The rise of Romboutsia in the intestinal tract caused by high fat diet is obviously relieved. The levels of anti-oxidative stress CAT and SOD in the liver are remarkably improved, serum lipopolysaccharide endotoxin is inhibited, and a colon barrier structure is maintained. The lactobacillus reuteri CCFM1145 disclosed by the invention can be used for preparing pharmaceutical compositions and fermented foods for relieving diseases such as metabolic syndrome, non-alcoholic fatty liver disease and diabetes mellitus and the like, and has a very wide application prospect.

The invention provides fish-meal-free Penaeus vannamei Boone compound feed for supplementing biological active peptide. The feed is prepared from shrimp meal, shrimp shell powder, chicken powder, meat meal, squid paste, bean pulp, flour, rice protein powder, rice concentrated protein powder, spray-dried animal blood cells, fish oil, soyabean lecithin oil, monocalcium phosphate, compound phagostimulant, biological active peptide, vitamin premix, mineral premix and choline chloride. By supplementing biological active peptide and amino acid, and the defect that generally, in the prior art, the ingestion speed is decreased when fish meal in shrimp feed formulas is reduced or does not exist is overcome.

The invention discloses an application of a compound Szeto-Schiller-31 (SS-31) to prepare a medicine for treating Friedreich's ataxia (FRDA) and related diseases. The invention belongs to the field of a medicine, and specifically relates to an application of SS-31 to treat FRDA diseases. It is found from experiments that SS-31 can up-regulate expression of frataxin (FXN) in FRDA patient's cells in a translational level, adjust iron metabolism in FRDA patient's cells, promote synthesis of iron-sulfur clusters in mitochondria, improve the function of mitochondria, reduce generation of ROS in patient's cells, and improve anti-oxidative stress performance of cells. The result shows that SS-31 has the potential value of treating FRDA.

The invention discloses black fungus melanin and preparation and application thereof. Deionized water and alkaline protease are added into black fungus powder, ultrasonic extraction and centrifugationare performed, and supernatant and precipitate are obtained; concentrating the supernatant to 1/3 of the original volume to obtain a supernatant concentrated solution; adding an extracting agent intothe precipitate, carrying out ultrasonic extraction and centrifugation, mixing the supernatant with the supernatant concentrated solution, and carrying out freeze drying to obtain black fungus melanin; the extraction yield of black fungus melanin can reach 12.09%, the removal rate of ABTS < + > is 91.47%, the removal rate of superoxide anion free radicals is 87.12%, the removal rate of hydrogen peroxide is 91.83%, the half lethal days of nematodes are 11.3 d, and the SOD activity and the CAT activity reach 36.2 U/mg and 45.1 U/mg respectively.

The present invention belongs to the field of aquatic feed, in particular relates to a special feed capable of improving the ingestion rate and immunity of bighead carp and silver carp early starters in spring. The feed particularly comprises the following components by weight: 15-20 parts of wheat bran, 15-20 parts of soybean cake, 10-15 parts of rice bran, 5-10 parts of fish meal, 5-10 parts of Antarctic krill powder, 5-8 parts of feed grade seaweed powder, 0.5-1 part of pounce, 1-2 parts of GuShu brand aquatic compound multivitamin type I, 1-2 parts of sodium alginate powder, 0.1-0.3 part of antioxidants, 5- 8 parts of plant additives and 0.02-0.1 part of sodium citrate. Among the antioxidants, beta glucan, vitamin E and glutathione have synergism, and can reduce the impact of external factor and stress on the growth performance, ingestion rate of bighead carp and silver carp in early spring, and improve the breeding survival rate and growth rate. The plant additives can increase the food intake of bighead carp and silver carp and improve the disease resistance of bighead carp and silver carp.

The invention provides a linear 23 peptide molecule. The linear 23 peptide molecule can be used for coupling a single B cell epitope or hapten to prepare immunomodulating peptide. The immunomodulatingpeptide immunizes animals and can produce an antibody with a high titer that can specifically bind to a protein containing a B cell epitope. A Th2 antibody or a single-chain antibody can be preparedby a hybridoma technology, or an antibody library or single-cell and binding genetic engineering and other technologies. The immunomodulating peptide and the antibody both significantly enhance the immunoregulation deflecting toward Th2, inhibit the activity or function of protease containing the B cell epitope, and improve related diseases. At the same time, the 23 peptide and a coupling or fusion protein or a composition/polymer thereof provided by the invention can also be used in recombinant microorganisms and transgenic animals or plants and cells thereof, so that the 23 peptide and the coupling or fusion protein or the composition/polymer thereof can be produced in production plants to produce vaccines or oral vaccines or produce specific antibodies, and the above peptides and antibodies can be used in the development of drugs to prevent and treat diseases with high Th1, including but not limited to diabetes, hyperuricemia and complications, as well as in the development of detection reagents to detect proteins or peptides containing B cell epitopes.

The invention discloses a soybean powder immersion liquid medium suitable for the growth and transformation of soybean isoflavone transformed strains. The medium comprises 0.5-2.5 wt% of lactose, 0.1-0.5 wt% of ammonium sulfate, 0.6-0.9 wt% of K2HPO4 and KH2PO4, 0.002-0.005 wt% of MnSO4, and the balance being soybean powder immersion base liquid; the ratio of the K2HPO4 to KH2PO4 is 1:4-2:1; and the mass concentration of the soybean powder immersion base liquid is 2%. According to the invention, cheap soybean vegetable proteins are used to replace expensive animal proteins such as cow brain, cow heart, cow liver and the like, thus greatly reducing the medium cost without influencing the transformation rate of the strains.

The invention relates to a method for improving antioxidant capacity and quality of seeds. In the method, the recombination of a seed-specific promoter At2S3 and three antioxidant genes is applied to improve the antioxidant capacity of the seeds. The upstream 1760 bp promoter sequence of the At2S3 gene respectively fuses GUS (beta-glucuronidase) reporter gene, manganese-superoxide dismutase (MSD1) gene, catalase (CAT1) gene and a gene of a key enzyme (homogentisate plant-transferase; HPT1) during vitamin E biosynthesis to transform arabidopsis and carry out strain hybridization. The results show that the GUS reporter gene is specifically and efficiently expressed in the seeds; and the monovalent MSD1 (M7), bivalent MSD1/CAT1 (M7/C9) and trivalent MSD1/CAT1/HPT1 (M7/C9/H3) transgenic arabidopsis strains have significantly higher germination rate of seeds when being subjected to 10 mu M paraquat oxidative stress treatment and significantly higher survival rate of seedlings when subjected to 1 mu M paraquat oxidative stress treatment than those of wild-type strains. Therefore, the promoter is a seed-specific highly-efficient promoter, and the recombination of the promoter and three antioxidant genes has important economic and social benefits for prolonging storage period of the seeds and improving quality and stress resistance of the seeds.

The invention discloses an antibiotic replacement type deodorizing feed microecological preparation as well as a preparation method and application thereof. The microecological preparation comprises following components in parts by weight: 200-300 parts of coated lactobacillus plantarum, 200-300 parts of coated enterococcus faecium, 20-50 parts of bacillus subtilis and 300-500 parts of fructo-oligosaccharide. The microecological preparation provided by the invention uses the coated lactobacillus plantarum and the coated enterococcus faecium, solves the problems of poor stress resistance of lactic acid bacteria, that is, short shelf life under a normal-temperature storage condition and easy inactivation in a feed granulation process. Besides, the microecological preparation disclosed by theinvention can reduce the content of odorous substances in animal waste, reduces the pollution of livestock and poultry breeding to the environment in the antibiotic-free breeding process, has the functions of promoting growth and deodorizing, and has important economic and social significance.

The invention discloses a feed additive for relieving pig stress and a preparation method thereof, and belongs to the technical field of feed additives, the feed additive for relieving pig stress comprises the following components in parts by weight: 25-30 parts of nano-gel coated traditional Chinese medicine powder, 7-10 parts of mixed powder, 2-3 parts of soybean lecithin, 1-3 parts of zinc oxide, and 0.5-1 part of glycerol monolaurate; the preparation method comprises the following steps: adding nano-gum coated traditional Chinese medicine powder, mixed powder, soya bean lecithin, zinc oxide and glycerol monolaurate into a stirring tank for stirring, obtaining a primary feed additive after stirring is finished, and drying the primary feed additive to obtain the feed additive for relieving pig stress after drying is finished. The feed additive disclosed by the invention can achieve a comprehensive stress prevention effect on various stress sources, and can also solve the problems that hormone or antibiotics are abused in the feed additive, some trace elements exceed the standard, the side effect is large, the action time is long, and the drug effect is slow.

The invention discloses an olea europaea leaf extractive feed additive and application thereof. The olea europaea leaf extractive feed additive is prepared by the method comprising the following steps: preparing olea europaea leaves which are naturally dried in the shade as the raw materials; preparing an ethyl alcohol-water green organic mixing system with certain concentration as an extracting solvent; quickly extracting under a room temperature with ultrasonic assistance; filtering and drying to obtain olea europaea leaf extractive rich in active ingredients. The olea europaea leaf extractive prepared by the method is used as the feed additive and capable of promoting animal growth, improving the resistance to oxidative stress as well as improving immunity; the olea europaea leaf extractive feed additive is applicable to the field of nonreactive animal feed additives.

The invention discloses a feed additive for protecting fish livers in case of oxidative stress and a preparation method. The feed additive is prepared from the following raw materials in percentage bymass: 2%-8% of tea polyphenols, 8%-15% of selenium, 20%-28% of vitamin C, 20%-25% of vitamin E, 15%-20% of radix glycyrrhizae, 10%-15% of giant knotweed rhizome, 5%-10% of lycium barbarum polysaccharides and the balance of filler. According to the feed additive for protecting the fish livers in case of oxidative stress and the preparation method, provided by the invention, the tea polyphenols canact a synergistic action with the selenium, the vitamin C, the vitamin E and Chinese herbal medicines such as the radix glycyrrhizae and the giant knotweed rhizome, so that the feed additive can be used for clearing away active oxygen radicals, improving oxidation resistance of organisms, adjusting free radical turbulence caused by feedstuff grease oxidation, promoting energy metabolism, restoring impaired liver cells, protecting the fish livers in case of oxidative stress and promoting the healthy growth of fishes.

The invention provides a grouper breeding heat stress resisting method. The method includes following steps of gradient stress resisting domestication, hungry domestication and preparation of an anti-stress agent. After groupers go through anti-stress treatment by the method, live oxidation stress resistance of heat stress resisting of the groupers is enhanced; by improving oxidation resistance ofbodies, oxidization stress of the bodies is reduced, environment adaptability of fishes is improved, and physique of the fishes is improved; the anti-stress agent prepared by the method can improve fish immunity, maintain blood balance and relieve autoimmunity generated by the fishes in a heat stress state.

The invention belongs to the field of food, and particularly relates to a composition for pediatric cancer patients to eat. The composition comprises protein, fat, carbohydrate, multi-vitamins, mineral elements and glutamine. In the composition, the fat serves as a main ingredient and provides calories, energy generated by cancer cells through a glucose anaerobic glycolysis path and essential groups of cancer cell DNA replication are blocked, and the aims of starving cancer cells and inhibiting cancer cell growth are achieved. According to the composition for pediatric cancer patients to eat,medium chain triglyceride contained in the composition can intensify the in-vivo ketogenic effect of patients, and child cancer cells are specificity starved. Intestinal-mucosa damage caused by chemotherapy drugs can be prevented and repaired through the added glutamine, an intestinal micro-ecosystem can be adjusted through added bifidobacterium triple viable bacteria, and an antitumor immunity mechanism of the body is activated; meanwhile, the composition has the high-energy density and the suitable osmotic pressure, malnutrition can be effectively improved, and the composition also has the intestinal-receptivity advantage, and is particularly suitable for child patients.

The invention belongs to the field of animal feed specially applicable to specific animals, and particularly relates to a feed additive for production of low-cholesterol eggs. The feed additive for the production of low-cholesterol eggs comprises the following components: tea polyphenols and yeast cultures. The additive significantly reduces the cholesterol content of eggs produced by laying hens,significantly improves the antioxidant resistance of the laying hens, and does not affect the egg yield, and the eggs produced have no odor.

The invention discloses a natural plant essential oil compound preparation. The natural plant essential oil compound preparation comprises the following raw materials in parts by mass: 10-40 parts ofherba pogostemosis essential oil, 5-25 parts of herba houttuyniae essential oil, 1-10 parts of citric acid, 35-65 parts of talcum powder, 15-45 parts of silicon dioxide, 0.1-2 parts of edible essences, 0.1-2 parts of a sweetener, and 0.1-2 parts of a flavoring agent. The raw materials of the natural plant essential oil compound preparation and the proportion thereof are optimized and adjusted so that the natural plant essential oil compound preparation has good palatability for livestock and poultry; and the natural plant essential oil compound preparation is especially beneficial for digestive system absorption of the livestock and poultry. Moreover, the natural plant essential oil compound preparation also has the functions of improving intestinal flora balance, reducing diarrhea, maintaining intestinal health, improving respiratory tract disease resistance, reducing influenza incidence, increasing feed intake, body weight gain and production performance, and enhancing anti-oxidativestress ability and immunologic functions.

The invention discloses use of lycium barbarum polysaccharides in preparation of medicines for dilatating a thoracic aorta. According to the lycium barbarum polysaccharides, the action of protecting acardiovascular system is exerted through improving expression of an oxidative-stress-resisting signal channel protein PKeap1/Nrf2, improving blood vessel endotheliocyte oxidative stress injury induced by Ang II (angiotensin II) and then improving an oxidative-stress-resisting state of the cardiovascular system. Simultaneously, the lycium barbarum polysaccharides further play a role in reducing inflammation causing factors.

The invention relates to an application of gastrodin to medical titanium metal usage in diabetes environment. After a medical material is implanted into a human body, tissue repair of a 'material-bone' interface mainly relates to integration of new bone tissue and materials, wherein the osseointegration effect of the materials is determined by bioactivity and function of osteoblast responsible forosteogenic differentiation on the materials. According to the application disclosed by the invention, through osteoblast adhesive culture on the surface of medical titanium metal, an in vitro 'titanium-bone' interface model is constructed; and the gastrodin of therapeutic dose is used for performing addition culture, so that the improvement effect of the gastrodin on the biological functions of the osteoblast in the 'titanium-bone' interface is proved. The clinical problems that in the prior art, oxidative stress damage is caused to the osteoblast in the 'titanium-bone' interface by the diabetes environment, biological functions of cells are influenced, apoptosis damage is worsened, and bad osseointegration of a titanium metal implant and loosening of the titanium metal implant after a long term are finally caused, are solved, and the stability of the medical titanium metal implant is improved.

The invention relates to the technical field of feed additives, in particular to a compound feed additive for improving salpingitis of laying ducks, which comprises the following components: antibacterial functional bacillus, lactobacillus plantarum, lysozyme, caulis spatholobi extract, sanguisorba officinalis extract, liquorice extract and kaolin, the feed has the advantage of remarkable effect of improving salpingitis of laying ducks.

The invention belongs to the technical field of lipid metabolism disorder prevention and treatment, and relates to a compound plant extract for preventing and treating lipid metabolism disorder and a preparation method thereof. The compound plant extract is prepared from the following raw materials in parts by weight: 5-15 parts of aralia elata, 5-15 parts of gentiana macrophylla, 5-15 parts of yellow tea Junshan silver needle, 4-10 parts of endive, 4-10 parts of burdock, 4-10 parts of Hangzhou celery, 3-8 parts of pomegranate flower, 3-8 parts of purslane, 3-8 parts of inula flower, 3-8 parts of lithocarpus polystachyus, 3-8 parts of moso bamboo leaf, 3-8 parts of kelp and 3-8 parts of enteromorpha. The compound plant extract for preventing and treating lipid metabolism disorder and the preparation method thereof disclosed by the invention have the beneficial effects that: an extract A and dry powder B are adopted to prepare the composition, so that the composition can be synergistically and mutually regulated to synthesize and decompose lipid; the synthesis of fatty acid is inhibited; the physiological processes of cholesterol absorption, triglyceride synthesis and secretion, plasma low-density lipoprotein oxidation and the like of a body are regulated and controlled; and finally, abnormal deposition of fat in all parts of the body is reduced, so that the purposes of preventing and treating fat metabolism disorder and related diseases or complications thereof are achieved.