Lactobacillus reuteri capable of intervening in metabolic syndrome and application

A technology of Lactobacillus reuteri and metabolic syndrome, applied in the field of microorganisms, can solve the problems such as the lack of research on probiotics in the intestinal barrier of the colon, and achieve the goal of improving the ability to resist oxidative stress, wide application prospects, and reducing fasting blood sugar levels. Effect

Inactive Publication Date: 2021-02-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use probiotics to alleviate metabolic syndrome, and there are few studies on how probiotics can inhibit endotoxin and protect the intestinal barrier of the colon.

Method used

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  • Lactobacillus reuteri capable of intervening in metabolic syndrome and application
  • Lactobacillus reuteri capable of intervening in metabolic syndrome and application
  • Lactobacillus reuteri capable of intervening in metabolic syndrome and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1 Screening and Identification of Lactobacillus reuteri CCFM1145

[0074] (1) Isolation and screening of lactic acid bacteria

[0075] (1) Take 1g of fresh feces from healthy people in Zhangye City, Gansu Province. After gradient dilution, it is spread on a plate of highly selective solid medium for Lactobacillus (each 1L MRS medium contains 0.02g vancomycin, 20g agar, pH value is 5.0±0.1), and cultured at 37°C for 48-72 hours.

[0076] (2) Observe and record the colony morphology, pick and streak the colonies for purification.

[0077] (3) Cultivate in MRS liquid medium at 37° C. for 24 hours, perform Gram staining on the obtained colonies, and record the colony morphology.

[0078] (4) Discard the Gram-negative bacterial strains and Gram-positive cocci in the colonies, and select the Gram-positive bacilli.

[0079] (5) After the catalase analysis, discard the catalase-positive strains and keep the catalase-negative strains.

[0080] (2) Molecular biologica...

Embodiment 2

[0095] Example 2: Lactobacillus reuteri CCFM1145 has good tolerance to simulated gastrointestinal fluid

[0096] Streak-inoculate the cryopreserved Lactobacillus reuteri CCFM1145 in MRS solid medium (MRS medium + 0.05% cysteine ​​hydrochloride), anaerobic static culture at 37°C for 48h, and then cultured by MRS After 2-3 subcultures, take 1ml of Lactobacillus reuteri CCFM1145 culture solution, mix it with 9.0mL pH 2.5 artificial simulated gastric juice (MRS medium containing 1% pepsin, pH=2.5), and incubate at 37°C. Under anaerobic culture, samples were taken at 0h, 0.5h, 1h and 2h, and MRS agar medium was poured for culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate. The survival rate is the ratio of the number of viable bacteria when sampling in the culture solution to the number of viable bacteria at the 0th hour, expressed in %.

[0097] Take 1ml of the culture solution of Lactobacillus reuteri CCFM1145 and add it to...

Embodiment 3

[0102] Example 3: Lactobacillus reuteri CCFM1145 has no toxic side effects on C57BL / 6J mice

[0103] Resuspend Lactobacillus reuteri CCFM1145 cells in normal saline to make a concentration of 1×10 10 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, 0.2 ml of the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0104] The results are listed in Table 3. Feeding concentration 1×10 10 CFU / mL of Lactobacillus reuteri CCFM1145 had no significant impact on the mice, the body weight of the mice had no significant change, the appearance of the mice had no obvious pathological symptoms, and no death occurred.

[0105] Table 3 Changes in body weight and death of mice

[0106]

[0107] Note: - means no death of mice.

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Abstract

The invention discloses lactobacillus reuteri capable of intervening in metabolic syndrome and application, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1145 screened by the invention can significantly improve the increase of serum total cholesterol content of metabolic syndrome mice caused by high fat diet. The weight rise of metabolic syndrome individuals is relieved, and the daily diet intake and the energy efficiency are remarkably reduced. Fasting blood glucose and insulin resistance index levels of metabolic syndrome individuals are significantly improved. The rise of Romboutsia in the intestinal tract caused by high fat diet is obviously relieved. The levels of anti-oxidative stress CAT and SOD in the liver are remarkably improved, serum lipopolysaccharide endotoxin is inhibited, and a colon barrier structure is maintained. The lactobacillus reuteri CCFM1145 disclosed by the invention can be used for preparing pharmaceutical compositions and fermented foods for relieving diseases such as metabolic syndrome, non-alcoholic fatty liver disease and diabetes mellitus and the like, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri capable of interfering with metabolic syndrome and its application, especially a strain capable of interfering with metabolic syndrome, which can be added to various health foods and health foods, and belongs to the technical field of microorganisms. Background technique [0002] Metabolic syndrome (MS) is a group of central obesity, hyperglycemia (diabetes or impaired glucose regulation), dyslipidemia (including high TG and / or low HDL-C blood), and hypertension. At the same time, there are multiple risk factors based on abnormal glucose metabolism and lipid metabolism that gather together to promote the occurrence and development of various cardiovascular and cerebrovascular diseases such as type II diabetes and atherosclerosis. In recent years, with the continuous development of my country's economic and social level, the living standards of the public have continued to improve, the proportio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P1/16A61P3/10A61P3/06A61P9/12A61P3/04A23L33/135A23L11/65A23C9/127A23L2/38C12R1/225
CPCC12N1/20A61K35/747A61P1/00A61P1/16A61P3/10A61P3/06A61P9/12A61P3/04A23L33/135A23C11/106A23C9/127A23L2/382A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/328A23V2200/3262A23V2200/32A23V2200/30A23V2200/3204A23V2200/326A23V2200/332
Inventor 陈卫杨波郑富莉赵建新张灏
Owner JIANGNAN UNIV
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