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Detection method of zearalenone in traditional Chinese medicine decoction pieces

A technology of zearalenone and traditional Chinese medicine decoction pieces, which is applied in the field of analytical chemistry, can solve the problems of inaccurate detection results, prolong the detection time, increase the detection cost, etc., and achieve the effect of low detection cost, simple detection process and short time consumption

Active Publication Date: 2016-09-28
SUZHOU TIANLING CHINESE TRADITIONAL MEDICINE SLICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many methods for detecting zearalenone in the prior art, but there are the following disadvantages: during the detection process, due to the interference of other toxins, the detection results are inaccurate; in addition, the detection process is more complicated, which prolongs the detection time , which increases the detection cost

Method used

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  • Detection method of zearalenone in traditional Chinese medicine decoction pieces

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A detection method for zearalenone in Chinese medicinal pieces, comprising the following steps:

[0019] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.2kW / h, a rotating speed of 2500 rpm, and an hourly input volume of 2kg of decoction pieces, pulverize and grind until powdery, and the particle size of the powder is 200 mesh;

[0020] (2) Soak the powder obtained in step (1) in sodium carbonate with a mass concentration of 0.5% for 0.5 hours, rinse with distilled water until neutral, and dry in an oven at 58°C;

[0021] (3) Extract the powder dried in step (2) with methanol at a mass concentration of 34%, shake the extract for 13 minutes, centrifuge at 4°C and 6000 rpm for 5 minutes, and take the supernatant;

[0022] (4) Adopt the supernatant in the high performance liquid chromatography detection step (3), mobile phase is the ammonium formate of 3mol / L and the formic acid of 0.2% mix by volume ratio 1:3, flow velocity is 0.3mL / min, column ...

Embodiment 2

[0024] A detection method for zearalenone in Chinese medicinal pieces, comprising the following steps:

[0025] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.6kW / h, a rotating speed of 2900 rpm, and an hourly input volume of 4 kg of decoction pieces, and pulverize and grind them into powder with a particle size of 350 mesh;

[0026] (2) Soak the powder obtained in step (1) in sodium carbonate with a mass concentration of 1.8% for 2.4 hours, rinse with distilled water until neutral, and dry in an oven at 66°C;

[0027] (3) Extract the powder dried in step (2) with methanol at a mass concentration of 46%, shake the extract for 24 minutes, centrifuge at 4°C and 7200 rpm for 7 minutes, and take the supernatant;

[0028] (4) Adopt the supernatant in high performance liquid chromatography detection step (3), mobile phase is the ammonium formate of 4.2mol / L and the formic acid of 0.3% mix by volume ratio 4:3, flow velocity is 0.7mL / min, column The tempe...

Embodiment 3

[0030] A detection method for zearalenone in Chinese medicinal pieces, comprising the following steps:

[0031] (1) Add Chinese herbal decoction pieces into a pulverizer with a power of 1.8kW / h, a rotating speed of 3200 rpm, and an hourly input volume of 5kg of decoction pieces, and pulverize and grind them into powder, with a particle size of 400 mesh;

[0032] (2) Soak the powder obtained in step (1) in sodium carbonate with a mass concentration of 2.5% for 3 hours, rinse with distilled water until neutral, and dry in an oven at 75°C;

[0033] (3) Extract the powder dried in step (2) with methanol with a mass concentration of 56%, shake the extract for 28 minutes, centrifuge at 4°C and 8000 rpm for 10 minutes, and take the supernatant;

[0034] (4) Adopt the supernatant in the high performance liquid chromatography detection step (3), the ammonium formate of mobile phase is 5mol / L and the formic acid of 0.4% mixes by volume ratio 5:3, flow velocity is 0.8mL / min, column tempe...

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Abstract

The invention discloses a detection method of zearalenone in traditional Chinese medicine decoction pieces, wherein the method comprises the steps of crushing, alkaline leaching, cleaning, extraction and high performance liquid chromatography detection. Compared with the prior art, the detection method has the following advantages: (1) the traditional Chinese medicine decoction pieces are pre-treated by the method, so that fungal walls are broken, so as to improve the accuracy of detection; and (2) the aflatoxin detection efficiency of the method is high.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for detecting mycotoxins, in particular to a method for detecting zearalenone in decoction pieces of traditional Chinese medicine. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by fungi. There are many kinds of them, which widely contaminate crops, food and feed, etc., and seriously threaten human health. They have attracted widespread attention worldwide. [0003] Zearalenone (ZEA for short), also known as F2 toxin, is mainly produced by Fusarium fungus. In the reproductive system, it has strong reproductive toxicity and teratogenic effects, and can cause infertility or abortion in animals. [0004] There are many types of traditional Chinese medicine and its related products, and the medicinal materials are grown in a wide range of areas. In the process of production, processing, transportation and storage, it is easy to mildew and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 李建华
Owner SUZHOU TIANLING CHINESE TRADITIONAL MEDICINE SLICE
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