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A method for quantifying an analyte, and an automatic analytical device configured to implement said method

An analyte and partner technology, applied in the field of automatic analysis devices, can solve the problems of inconvenient use of evaporation equipment, toxic organic solvents, etc., and achieve the effect of cost-effectiveness and reduction of completion time

Active Publication Date: 2016-10-26
IMMUNODIAGNOSTIC SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the analyte purification steps performed prior to quantification may require the use of organic solvents, which can be toxic and may require evaporation equipment, which is inconvenient to use in clinical biochemistry laboratories

Method used

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  • A method for quantifying an analyte, and an automatic analytical device configured to implement said method
  • A method for quantifying an analyte, and an automatic analytical device configured to implement said method
  • A method for quantifying an analyte, and an automatic analytical device configured to implement said method

Examples

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example 1

[0115] Example 1: Measuring the amount of 1.25D concentration in a sample according to the invention

[0116] The measurement of 1,25D in human blood is a very good indicator of the effectiveness of the body's vitamin D metabolism.

[0117] It has been difficult to develop assays for determining 1,25D levels, mainly because the concentration of 1,25D in blood is very low.

[0118] It is well known that laborious multiple extraction steps are required to measure 1,25D before analysis on automated systems or by manual methods. Existing extraction methods available on the market today require extensive equipment including purification columns, spinners, centrifuges and nitrogen evaporators. Often a solvent is required. Positive identification of the sample will be compromised.

[0119] The measurement of 1,25D in a sample according to the invention begins with the pretreatment of the sample for 1,25D by degreasing the sample in a first cuvette. Defatting was performed using...

example 2

[0126] Example 2: Measure the amount of 1.25D concentration in the sample according to a previously known method

[0127] Samples were decontaminated in tubes by adding 500 μL of sample to labeled glass or plastic test tubes, followed by 50 μL of degreaser (comprising 10 g of dextran sulfate (50k) in one liter of 0.5 M magnesium chloride, Sigma catalog number D8787). Degrease. Mix and centrifuge at 2000g for 15 minutes.

[0128] Label the capsules. Remove capsule screw cap. Add 150 μL of defatted sample to a capsule containing a suspension of a solid phase to which a monoclonal antibody highly specific for 1,25D is attached. Securely return the nut to its place. The capsules were turned up and down for 90 minutes at room temperature to allow 1,25D to bind to the mAb.

[0129] Allow the capsules to stand upright for 3 to 5 minutes to allow the gel to settle. Remove the screw cap and free the bottom plug from the capsule. Place each capsule in a glass or plastic tube an...

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Abstract

The present invention deals with a novel method for determining the amount of an analyte in a sample comprising an initial purification step, occurring in a first container, comprising the following steps of mixing the sample, a delipidation agent and magnetic particles coated with first analyte binding partners in the first container, incubating the mix, removing the unbound reagents from the mix, and eluting the bound analyte in an elution solution; a transferring step consisting transferring in a volume of the elution solution comprising the analyte from the first container to a second container; and a quantification step, occurring in the second container, consisting of quantifying the analyte in said elution solution.

Description

technical field [0001] The present invention relates to a novel method for determining the amount of an analyte in a sample, and more particularly to a method utilizing an immunoassay and an automated analysis device configured to carry out the method. Background technique [0002] The present invention relates to a method for quantifying the amount of an analyte present in a sample, in particular a method enabling the quantification of the analyte without prior complex and laborious pre-analytical stages of sample purification. [0003] The present invention also relates to methods of determining the amount of an analyte useful in diagnosing a disease. [0004] Simplified extraction and separation methods are key features of the improved method. Requiring a separate purification step significantly lengthens the method and, since there is at least one step of manually manipulating the sample, introduces potential errors not only in determining concentrations but also in sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/82G01N33/543
CPCG01N33/54326G01N33/743G01N33/82G01N33/54333G01N33/92G01N35/0098G01N35/025G01N2035/0446G01N2035/0465
Inventor 杰奎琳·特兰诺贝特·布吕特洛伊克·科尔诺
Owner IMMUNODIAGNOSTIC SYST
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