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Method for synthesizing phlebopus portentosus cavity insect galls

A technology of boletus coelomium and dark brown netstalk, which is applied in the field of synthesis of boletus boletus boletus cavity galls, and can solve the problem of difficulty in artificially synthesizing boletus boletus cavity galls and bacterial cavity galls low infection rate

Inactive Publication Date: 2016-11-09
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Boletus coeloid galls are the first step in the biomimetic cultivation of dark brown reticulum boletus liver, but it is currently difficult to artificially synthesize boletus coelom galls, or the infection rate of coelom galls is very low when artificially synthesized

Method used

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Examples

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Effect test

preparation example Construction

[0022] The synthetic method of Dictyostelium phalaenopsis fungus cavity gall of the present invention comprises the following steps:

[0023] (1) The solid original species of Dictiostelium boletus, provided by the Boletus project of the Plant Protection and Microbiology Research Center of Yunnan Institute of Tropical Crops Science;

[0024] (2) Planococcus minor, picked from the fungus galls on the roots of coffee trees, and fed with pumpkins to multiply.

[0025] (3) Cultivate the coffee seeds to the point of germination, transplant into the aseptic seedling medium and cultivate to obtain the aseptic seedlings;

[0026] (4) When the aseptic seedlings grow lateral roots, inoculate the solid stock of Dictyostelium dunciscens and mealybug in its root system, keep the substrate moist, maintain the temperature at 28°C±2°C, and cultivate to obtain Boletus duncistelium. Hepatic fungus coelom galls.

[0027] The solid original species of Dictiostelium boletus selected in the prese...

Embodiment 1

[0036] (1) The solid original seed of Dictyostelium brucei was provided by the Boletus project of the Plant Protection and Microbiology Research Center of Yunnan Institute of Tropical Crops Science. The strain number is: 13013. Check the quality of the bacteria, the mycelium is covered with bacteria bags, yellowish brown, dense, strong, uniform growth, consistent color, no drying shrinkage, and the bacteria that are close to the bacteria bag are qualified strains;

[0037] (2) Spread a layer of filter paper on the bottom of the tissue culture bottle, soak it with tap water, then tie it with a wrapping paper, sterilize at 121°C for 30 minutes, and cool it for later use;

[0038] (3) Select mature coffee seeds, wash them with tap water for 30 minutes, and wash them with 3% H 2 o 2 Soak for 5 minutes, while constantly shaking, and then rinse with sterile water three times. Put it into a sterilized tissue culture bottle, put 4 mature seeds in each bottle, place it in an environm...

Embodiment 2

[0045](1) The number of the solid original species of Dictyostelium brucei is: 13013. The strain is provided by the Boletus Project of the Plant Protection and Microbiology Research Center of the Yunnan Institute of Tropical Crops Science. The culture time in the culture room is 50 days. Check the quality of the bacteria beforehand. The mycelia are covered with bacteria bags, yellowish brown, dense, strong, uniform in growth, consistent in color, without drying shrinkage, and the bacteria that are close to the bacteria bag are qualified bacteria;

[0046] (2) Spread a layer of filter paper on the bottom of the tissue culture bottle, soak it with tap water, then tie it with a wrapping paper, sterilize at 121°C for 30 minutes, and cool it for later use;

[0047] (3) Select mature coffee seeds, wash them with tap water for 30 minutes, and wash them with 3% H 2 o 2 Soak for 5 minutes, while constantly shaking, and then rinse with sterile water three times. Put it into a steriliz...

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Abstract

The invention relates to the field of bionic cultivation of fungi, in particular to a method for synthesizing phlebopus portentosus cavity insect galls. The method comprises the steps of 1, providing solid protospecies of phlebopus portentosus by the bolete project of plant protection and micro-organism research center of Yunnan Province tropical crops scientific institute; 2, picking mealybug from fungus cavity insect galls of wild host tree roots, and conducting feeding and propagation expansion with pumpkin; 3, cultivating aseptic seedlings of host trees; 4, inoculating solid inoculum of phlebopus portentosus and mealybug in the root systems of aseptic seedlings after lateral roots come out of the aseptic seedlings, keeping a medium moist, maintaining temperature at 28+ / -2 DEG C, and conducting cultivation to obtain the phlebopus portentosus cavity insect galls. By the adoption of the method, the symbiotic relationship of phlebopus portentosus and host trees can be effectively established, the phlebopus portentosus cavity insect galls can be synthesized successfully, and phlebopus portentosus cavity infection rate is as high as 83-90%.

Description

technical field [0001] The invention relates to the field of biomimetic cultivation of fungi, in particular to a method for synthesizing the fungus coelom galls of Dictyostelium punctatus. Background technique [0002] Phlebopus portentosus (Berk. & Broome) Boedijn, also known as giant boletus, commonly known as "black boletus", belongs to the genus Phlebopus portentosus of the family Boletaceae of the order Boletus. Distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand and other regions. It is mainly distributed in Yunnan, Guangxi and Hainan. Its fruiting body is huge, the meat is thick, the stalk is strong, and it is rich in nutrients. It is rich in protein, potassium, calcium, magnesium, iron, zinc and other mineral elements needed by the human body. Its price is several times or ten times that of ordinary edible fungi. , has high economic value. [0003] The young and tender fruiting bodies of the dark brown reticulum boletus ar...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 刘静曹旸张春霞何明霞王文兵许欣景高锋方艺伟
Owner YUNNAN INST OF TROPICAL CROPS
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