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Application of stilbenoids in quorum sensing system for inhibiting chromobacterium violaceum

A quorum sensing system and the technology of stilbene compounds are applied in the field of medicine to achieve the effect of inhibiting the production of purple pigments

Inactive Publication Date: 2016-11-09
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Stilbene compounds are antibacterial phytoalexins that widely exist in the plant kingdom. At present, the research on stilbene compounds is focused on their biological activities such as antibacterial, anti-oxidation and anti-cancer, but whether they have QSI activity against Chromobacter violaceum still not reported

Method used

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  • Application of stilbenoids in quorum sensing system for inhibiting chromobacterium violaceum
  • Application of stilbenoids in quorum sensing system for inhibiting chromobacterium violaceum
  • Application of stilbenoids in quorum sensing system for inhibiting chromobacterium violaceum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Quorum sensing inhibitory activity judgment experiment of three stilbene compounds.

[0017] Prepare LB liquid medium and divide it into test tubes, prepare LB solid medium, sterilize and dry LB liquid medium, solid medium, pipette tips, petri dishes, centrifuge tubes, paper pieces, etc. for later use. Pick a single colony of CV026 that has been streak cultured, inoculate it into LB liquid medium, and culture it in a constant temperature shaker at 30°C overnight. Use LB liquid medium as a blank reference, and use sterilized LB liquid medium to make the OD of the overnight bacterial solution 620 Adjusted to around 0.1. Pour 100 mL of melted LB solid medium evenly into 6 plates as the lower agar plate. After the lower agar plate solidifies, take the OD 620 =0.1 CV026 bacterial solution was added to 100mL of cooled unsolidified LB solid medium (about 50°C), and signal molecule C6-HSL and kanamycin were added at the same time, mixed vigorously and evenly poured on the su...

Embodiment 2

[0020] Three stilbene compounds inhibit the growth of Chromobacter violaceum CV026 and the production of purple pigment.

[0021] The prepared LB liquid medium was divided into Erlenmeyer flasks and sterilized for later use. Pick a single colony of CV026 that has been streak cultured, inoculate it into LB liquid medium, and culture it in a constant temperature shaker at 30°C overnight. Add C6-HSL (signal molecule), overnight bacterial solution and various concentrations of test compounds (final concentrations 25, 50, 75, 100, 125 and 150 μM) to sterilized LB liquid medium, and use DMSO as a blank control . After culturing for 16-18 hours, pipette 1 mL×6 to 6 1.5 mL centrifuge tubes for each bottle. Discard the supernatant by centrifugation, resuspend the pellet with DMSO, suspend it sufficiently and centrifuge, pipette 200 μL of the supernatant into a 96-well plate to measure OD 585 , with DMSO as blank control. According to OD 585 To judge the effect of three stilbene co...

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Abstract

The invention discloses application of stilbenoids in a quorum sensing system for inhibiting chromobacterium violaceum. The stilbenoids comprise three stilbenoids, namely, resveratrol, piceatannol and oxyresveratrol. In the chromobacterium violaceum, generation of purple pigment is controlled by quorum sensing. The three stilbenoids of resveratrol, piceatannol and oxyresveratrol can all significantly inhibit quorum sensing of the chromobacterium violaceum under the sublethal concentration condition, namely, the condition without influencing growth of the chromobacterium violaceum, wherein the activity of resveratrol is the highest. The three stilbenoids of resveratrol, piceatannol and oxyresveratrol can be prepared into a quorum sensing inhibitor to the chromobacterium violaceum, can effectively inhibit quorum sensing and prevent bacteria infection and has an important practical value in clinic bacteriostasis application.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to the application of stilbene compounds in inhibiting the quorum sensing system of Chromobacterium violaceum, in particular to the application of three stilbene compounds, resveratrol, piceatanol and oxidized resveratrol, in inhibiting the population of Chromobacterium violaceum applications in sensing systems. Background technique [0002] Bacteria can produce, release and detect a chemical signal molecule called autoinducers (AI), the concentration of this signal molecule increases with the increase of bacterial population density, according to the change of signal molecule concentration, bacteria can detect to the changes in the concentration of the surrounding bacterial flora. When the concentration of signaling molecules reaches a certain threshold, bacteria can communicate and coordinate group behavior and regulate gene expression. This chemical communication mechanism of ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P31/04A01N31/16A01P1/00
CPCA01N31/16A61K31/05
Inventor 贾爱群陈彤彤周金伟盛吉洋
Owner NANJING UNIV OF SCI & TECH
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