Novel preparation method of bacteriostatic agent of hexahydro beta-acid cyclodextrin inclusion compound

Abstract

The invention relates to a preparation method of a water-soluble natural bacteriostatic agent, and belongs to the application field of natural product active ingredients. Particularly, the invention relates to a preparation method of a water-soluble cyclodextrin using a derivative of hexahydro beta-acid of beta-acid in lupulus. According to the method, nine kinds of different cyclodextrin including hexahydro beta-acid, alpha-cyclodextrin, beta-cyclodextrin, gamma-cyclodextrin and derivatives thereof and the like are used for inclusion; the cyclodextrin inclusion compound capable of improving the water solubility of the hexahydro beta-acid is obtained; according to requirements, the compound can be used in food proportioning, beverage proportioning, cosmetics, washing and caring articles and feed products for improving the anti-corrosion performance and the health care effect of the products.

Description

technical field

[0001] The invention relates to a preparation method of a water-soluble antibacterial agent, which belongs to the application field of natural plant active ingredients. More specifically, the present invention relates to a method of inclusion of hexahydro β-acid with 9 different cyclodextrins such as α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin and its derivatives to obtain Cyclodextrin inclusion complexes capable of improving the water solubility of hexahydro β-acids. The water solubility of the hexahydrobeta-acid cyclodextrin inclusion compound obtained by this method is obviously improved, and it can be added to various beverages, candies, jellies, biscuits, cakes as an antibacterial and antiseptic component, and can also be used in cosmetics, washing and nursing In supplies and feed products, at the same time, because hexahydro β-acid itself has various pharmacological activities, it can also be used as an ingredient of health products. Background tech...

Examples

Embodiment 1

[0026] Mix 0.05g of hexahydro β-acid with 2g of α-cyclodextrin, put it in a mortar, add a small amount of distilled water to make a paste, grind it for 1 hour, and dry it in an oven at 40-60°C until constant Heavy, to get six hydrogen β-acid-α-cyclodextrin inclusion complex. After analysis, the yield of inclusion compound was 94.6%, and the inclusion rate was 11.3%.

Embodiment 2

[0028]Mix 0.1g of hexahydro β-acid and 5g of β-cyclodextrin evenly, put them in a mortar, dry grind for 2 hours without adding any solvent, and obtain hexahydro β-acid-β-cyclodextrin The clathrate, after analysis, the yield of the clathrate is 96.13%, and the inclusion rate is 17.34%.

Embodiment 3

[0030] Mix 0.2g of hexahydro β-acid and 8g of hydroxypropyl-β-cyclodextrin evenly, put it in a mortar, add a small amount of deionized water to make a paste, grind for 1.5h, put it in an oven at 40-60 Under the condition of ℃, it was dried at low temperature to constant weight to obtain hexahydroβ-acid-hydroxypropyl-β-cyclodextrin inclusion compound. After analysis, the yield of inclusion compound was 97.32%, and the inclusion rate was 35.41%.

technical field

[0001] The invention relates to a preparation method of a water-soluble antibacterial agent, which belongs to the application field of natural plant active ingredients. More specifically, the present invention relates to a method of inclusion of hexahydro β-acid with 9 different cyclodextrins such as α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin and its derivatives to obtain Cyclodextrin inclusion complexes capable of improving the water solubility of hexahydro β-acids. The water solubility of the hexahydrobeta-acid cyclodextrin inclusion compound obtained by this method is obviously improved, and it can be added to various beverages, candies, jellies, biscuits, cakes as an antibacterial and antiseptic component, and can also be used in cosmetics, washing and nursing In supplies and feed products, at the same time, because hexahydro β-acid itself has various pharmacological activities, it can also be used as an ingredient of health products. Background tech...

Examples

Embodiment 1

[0026] Mix 0.05g of hexahydro β-acid with 2g of α-cyclodextrin, put it in a mortar, add a small amount of distilled water to make a paste, grind it for 1 hour, and dry it in an oven at 40-60°C until constant Heavy, to get six hydrogen β-acid-α-cyclodextrin inclusion complex. After analysis, the yield of inclusion compound was 94.6%, and the inclusion rate was 11.3%.

Embodiment 2

[0028]Mix 0.1g of hexahydro β-acid and 5g of β-cyclodextrin evenly, put them in a mortar, dry grind for 2 hours without adding any solvent, and obtain hexahydro β-acid-β-cyclodextrin The clathrate, after analysis, the yield of the clathrate is 96.13%, and the inclusion rate is 17.34%.

Embodiment 3

[0030] Mix 0.2g of hexahydro β-acid and 8g of hydroxypropyl-β-cyclodextrin evenly, put it in a mortar, add a small amount of deionized water to make a paste, grind for 1.5h, put it in an oven at 40-60 Under the condition of ℃, it was dried at low temperature to constant weight to obtain hexahydroβ-acid-hydroxypropyl-β-cyclodextrin inclusion compound. After analysis, the yield of inclusion compound was 97.32%, and the inclusion rate was 35.41%.