A kind of water body chlorophyll concentration in-situ detection device and its detection method

Abstract

The invention relates to an in-situ detection device for the chlorophyll concentration of water and a detection method of the in-situ detection device. The in-situ detection device and the detection method aim at solving the problems that in an existing device, the excitation light path can be not adjusted, the fluorescence intensity is low, and the fluorescence detection accuracy and sensitivity are low due to scattering generated when water contains lots of impurities. A water sample chamber water inlet device is arranged at the bottom of a quartz water sample chamber, a stainless steel shell is arranged outside the quartz water sample chamber, a detector, a light filter and a focusing lens are arranged over the quartz water sample chamber, a light source, a light source transverse adjustment support, a light source transverse adjustment mechanism and a light source direction adjustment mechanism are arranged on the side face of the quartz water sample chamber, and the detector, the light source, the light source transverse adjustment mechanism and a light source incidence angle adjustment mechanism are connected with a host through wires. The method includes the steps that water background fluorescence signals measured under the off-state of the excitation light source are collected, then water fluorescence signals under the on-state of the excitation light source are collected, and background fluorescence interference is automatically subtracted through operation.

Description

technical field [0001] The invention relates to a chlorophyll concentration detection device and a detection method thereof. Background technique [0002] At present, there are three main detection methods of chlorophyll in water: spectrophotometry, high performance liquid chromatography and fluorescence analysis. The detection of spectrophotometry and high performance liquid chromatography requires sampling and filtration, 90% acetone grinding and extraction, and centrifugation to take the supernatant for testing. In order to prevent the sample from being acidified and destroying the pigment, magnesium carbonate needs to be added to it. The process is cumbersome and relies on large equipment. It is only suitable for laboratory testing, not suitable for field operations and real-time monitoring. At present, most of the existing fluorescence analysis chlorophyll detection devices use a fixed blue LED as the excitation light source to excite chlorophyll to generate 685nm fluo...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide an in-situ detection device and detection method for chlorophyll concentration in water bodies; to solve the problem that existing devices adopt 180° or 90° optical path design, the excitation light path cannot be adjusted, and the generated fluorescence intensity is weak

When there are many impurities in the actual water body, scattering will occur, resulting in high noise background, low accuracy and sensitivity of fluorescence detection

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