Reagent clamping box with magnetic bead transfer structure

Abstract

Provided is a reagent clamping box with a magnetic bead transfer structure. The reagent clamping box comprises a first cavity, a second cavity and a spacing region, wherein the first cavity and the second cavity are adjacently arranged, and the spacing region spaces the first cavity from the second cavity; a containing cavity is formed in the spacing region, the magnetic bead transfer structure capable of transferring a magnetic bead in the first cavity into the second cavity is arranged in the containing cavity, the magnetic bead transfer structure is embedded in the containing cavity in a sealed mode, the magnetic bead transfer structure comprises a cylindrical rotating structure and a screw rod pushing the cylindrical rotating structure to rotate, and the screw rod pushes the cylindrical rotating structure to rotate in the containing cavity to achieve transfer of the magnetic bead. According to the reagent clamping box with the magnetic bead transfer structure, the magnetic bead transfer structure is arranged between the two adjacent cavities, and the magnetic bead and a to-be-extracted material attracted by the magnetic bead can be fully and efficiently transferred; in addition, the reagent clamping box is simple in self structure and convenient to operate, and the complexity of equipment matched with the reagent clamping box for use can be reduced.

Description

technical field [0001] The invention relates to consumables used in the preparation and detection of nucleic acid or protein, in particular to a reagent cartridge provided with a magnetic bead transfer structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip ...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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