Selective recognition method of calixyridine for acidic amino acids in neutral physiological pH aqueous solution
An acidic amino acid, calixpyridine technology, applied in the field of supramolecular recognition, achieves the effect of wide application value and efficient and simple characterization method
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Embodiment 1
[0031] A method for selective recognition of acidic amino acids by calixyridine in a neutral physiological pH aqueous solution, characterized in that the steps are as follows:
[0032] 1) Dissolve calixyridine and pyrene tetrasulfonic acid tetrasodium salt in water and mix uniformly to obtain a solution. The concentrations of calixyridine and pyrene tetrasulfonic acid tetrasodium salt are 0.004 mmol / L and 0.001 mmol / L respectively, and then The solution was tested for fluorescence at room temperature;
[0033] 2) Add 20 kinds of natural amino acids to the above solution respectively, the amino acid concentration is 1 mmol / L, and the amino acids include neutral amino acids alanine, valine, leucine, isoleucine, methionine , phenylalanine, serine, threonine, asparagine, glutamine, tyrosine, tryptophan, cysteine, proline, glycine, basic amino acid histidine, arginine, Lysine and acidic amino acids glutamic acid, aspartic acid. One minute after adding the amino acid, the resultin...
Embodiment 2
[0036] A method for selective recognition of acidic amino acids by calixyridine in neutral physiological pH aqueous solution:
[0037] 1) The selective recognition of acidic amino acids by calixyridine in neutral physiological pH aqueous solution was characterized by fluorescence spectroscopy, figure 1 It is the fluorescence emission spectrogram of calixyridine-pyrene tetrasulfonic acid tetrasodium salt, calixyridine-pyrene tetrasulfonic acid tetrasodium salt-amino acid aqueous solution, wherein the concentration of calixyridine is 0.004 mmol / L, and the concentration of pyrene tetrasulfonic acid tetrasodium salt is 0.001 mmol / L, amino acid concentration is 1 mmol / L, amino acids include acidic amino acids glutamic acid, aspartic acid, basic amino acids histidine, arginine, lysine and neutral amino acids alanine, valine Acid, Leucine, Isoleucine, Methionine, Phenylalanine, Serine, Threonine, Asparagine, Glutamine, Tyrosine, Tryptophan, Cysteine, Proline acid, glycine; the figu...
Embodiment 3
[0040] Example 2 The method for selective recognition of acidic amino acids by pyridine in neutral physiological pH aqueous solution is applied to the detection of peptide Glu-Glu formed by condensation of acidic amino acids, the method is as follows:
[0041] Calixyridine and pyrene tetrasulfonic acid tetrasodium salt were dissolved in water and mixed uniformly to obtain a solution. The concentrations of calixyridine and pyrene tetrasulfonic acid tetrasodium salt were 0.004 mmol / L and 0.001 mmol / L respectively. Such as Figure 7 As shown, as the peptide Glu-Glu synthesized by the condensation of acidic amino acids was gradually added to the calixyridine-pyrene tetrasulfonic acid tetrasodium salt solution, the fluorescence signal of pyrene tetrasulfonic acid tetrasodium salt gradually changed from weak fluorescence emission signal to strong fluorescence emission signal. Fluorescence emission signal, thereby realizing the application of the fluorescence sensing detection of the...
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