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Application of Tenascin-C in preparation of kidney injury diagnosis and treatment preparations

1. Tenascin-c, kidney damage technology, applied in the field of biomedicine, can solve problems such as morbidity and mortality endangering human health

Inactive Publication Date: 2016-12-07
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2013, cJASN published a systematic review on the incidence of AKI, and the results showed that: based on the AKI diagnostic criteria provided by the KDIGO guidelines, the incidence of AKI in hospitalized patients was 23.2% (95% CI 21.0-25.7%), and the all-cause mortality was 23.0 % (21.3-24.8%); such high morbidity and mortality not only endanger human health, but also bring huge burdens to society and the government
Although AKI can recover and even creatinine drops to the baseline level, a large proportion of patients cannot fully recover and transform into CKD, which is manifested by the progressive progression of renal interstitial fibrosis
At present, there is still a lack of effective biomarkers for detecting the recovery of AKI and whether there is residual renal interstitial fibrosis, and there is a lack of effective drugs to promote the regeneration of renal tubular epithelial cells after renal tubular necrosis

Method used

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  • Application of Tenascin-C in preparation of kidney injury diagnosis and treatment preparations
  • Application of Tenascin-C in preparation of kidney injury diagnosis and treatment preparations
  • Application of Tenascin-C in preparation of kidney injury diagnosis and treatment preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Detection of Tenascin-C in kidney tissue, serum and urine, reflecting the severity of renal interstitial fibrosis

[0039] 1. Clinical research

[0040] Paraffin sections of renal tissues (n=5) obtained from renal biopsies in our center and confirmed to have renal interstitial fibrosis were subjected to TNC immunofluorescent staining to observe the expression of TNC in fibrotic renal tissues. ELISA method was used to detect the level of Tenascin-C in serum and urine.

[0041] 2. Experimental animals

[0042] To Study the Effect of TNC Gene Deletion on Renal Interstitial Fibrosis Using Littermate TNC-CreER-eGFP + / + (i.e. TNC - / - ) mice and wild-type mice, TNC-CreER-eGFP was used for qualitative research on TNC expressing cells by fluorescent co-staining + / -Lineage tracing of mouse, TNC-expressing cells using TNC-CreER + / - ;R26-tdTomato + / - mice. All mice were of the 129 strain and were raised in an SPF-grade environment, the indoor temperature was maintained at 25...

Embodiment 2

[0047] Example 2 inhibits the function of Tenascin-C and alleviates the progress of chronic renal interstitial fibrosis

[0048] 1. Evaluation of chronic renal interstitial fibrosis

[0049] The blinded method was used by experienced pathologists. Masson staining was used to score 0-10 according to the severity of fibrosis in the cross-section of the mouse kidney, and the degree of fibrosis in the kidney was semi-quantified; immunohistochemistry was performed on the cross-section of the mouse kidney using the marker F4 / 80 of macrophages Staining, scanning with ImageJ software, and evaluating the infiltration degree of macrophages by comparing the size of the positive area; performing immunofluorescence staining on the cross-section of the mouse kidney with FSP-1, randomly selecting 20 fields of view under a 100-fold field of view, and calculating the small The number of FSP-1 positive cells in the cross-section of the mouse kidney; the mRNA and protein of the whole kidney wer...

Embodiment 3

[0054] Example 3 Detection of Tenascin-C in kidney tissue, serum and urine, reflecting the severity of acute kidney injury

[0055] 1. Clinical research

[0056] Acute tubular necrosis (ATN) renal tissue paraffin sections (n=5) obtained from renal biopsy in our center were subjected to TNC immunofluorescence staining to observe the expression of TNC in ATN renal tissue. ELISA method was used to detect the level of Tenascin-C in serum and urine.

[0057] 2. Experimental animals

[0058] To study the increased expression of TNC mRNA after renal IR using TNC-CreER-eGFP + / - In mice, TNC-CreER-eGFP was used to study the increase of TNC expression cells and the increased position after IR + / - Mice and TNC-CreER + / - ;R26-tdTomato + / - Mice to study the effect of TNC gene deletion on IR renal injury using littermate TNC-CreER-eGFP + / - (i.e. TNC - / - ) and wild-type mice. All mice were of the 129 strain and were raised in an SPF-grade environment, the indoor temperature was maint...

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Abstract

The invention belongs to the technical field of biomedicines, and relates to an application of Tenascin-C in the preparation of kidney injury diagnosis and treatment preparations. The Tenascin-C is used as a kidney injury or kidney fibration biomarker, and especially used as a biomarker in kidney injury or kidney fibration blood and urea. The invention provides an application of the Tenascin-C in the preparation of kits for detecting, diagnosing and treating kidney injury or kidney fibration. The biomarker indexes of the blood and the urea are monitored to reflect the severe degree of the kidney injury and predicate the kidney recovery opportunity, and intervention measures for promoting restoration of acute kidney injury and mitigation of the severe degree of the kidney injury are provided; and the biomarkers of the blood and the urea are monitored to reflect the severe degree of the kidney fibration, and intervention measures for facilitating early stage diagnosis of chronic kidney fibration and monitoring of the kidney fibration progress are provided.

Description

technical field [0001] The technical field of biomedicine of the present invention relates to the new medical application of Tenascin-C, relates to the application of Tenascin-C in the preparation of preparations for diagnosing and treating kidney damage, and specifically relates to the use of Tenascin-C in preparing and repairing acute kidney damage and diagnosing and treating chronic kidney damage. Application in the preparation of renal interstitial fibrosis disorders. Background technique [0002] Studies have shown that with the aging of the population, the incidence and mortality of chronic kidney disease (CKD) are also increasing year by year, which is consistent with the traditional factors of CKD including the prevalence of diabetes, hypertension and cardiovascular disease It is closely related to the year-on-year increase. According to the survey, the prevalence rate of CKD in American adults is 11%, and it is as high as 39.4% among people over 60 years old. Stati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P13/12G01N33/68
Inventor 郝传明谢琼虹张敏刘少军尚达王嘉关熠茅晓意
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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