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Composite micro-ecological water quality regulator and preparation method thereof

A water quality conditioner and composite micro-ecological technology, applied in the field of aquaculture technology and microorganisms, can solve the problems of high mortality of farmed animals, excessive chemical residues, frequent occurrence of diseases, etc., and the steps are easy to operate, repair intestinal epithelial tissue, Mitigate the effects of sudden changes in weather

Inactive Publication Date: 2016-12-07
XINYANG AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of water quality deterioration, water pollution and frequent disease occurrence in the existing aquaculture process, which may easily cause high mortality of farmed animals and excessive chemical residues in the body. Composite micro-ecological water quality regulator and preparation method thereof

Method used

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  • Composite micro-ecological water quality regulator and preparation method thereof
  • Composite micro-ecological water quality regulator and preparation method thereof
  • Composite micro-ecological water quality regulator and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of a composite micro-ecological water quality regulator, comprising the steps of:

[0024] (1) Preparation of preserved bacteria liquid

[0025] First prepare the pure strains of EM bacteria, Bacillus licheniformis, and Bifidobacterium, and the bacterial content of the bacteria liquids is diluted to 1×10 10 CFU / mL, 50mL per tube is distributed to each preservation tube, 40% glycerin is added as a protective agent, and it is stored at -20°C. The whole process requires aseptic operation.

[0026] (2) Preparation of fermented seed liquid

[0027] A 100L fermentation system was used to prepare the fermented seed liquid, and the seed culture medium of EM bacteria, Bacillus licheniformis, and Bifidobacterium were prepared respectively, and added to a 100L fermenter with a liquid volume of 70%, sterilized, and cooled to room temperature. Take one preserved EM bacteria, Bacillus licheniformis, and Bifidobacterium respectively, and inoculate them into the ...

Embodiment 2

[0037] A preparation method of a composite micro-ecological water quality regulator, comprising the steps of:

[0038] (1) Preparation of preserved bacteria liquid

[0039] First prepare the pure strains of EM bacteria, Bacillus licheniformis and Bifidobacterium, and dilute the bacteria content to 1×10 10 CFU / mL, 50mL per tube is distributed to each preservation tube, 40% glycerin is added as a protective agent, and it is stored at -20°C. The whole process requires aseptic operation.

[0040] (2) Preparation of fermented seed liquid

[0041] A 100L fermentation system was used to prepare the fermented seed liquid, and the seed culture medium of three kinds of microorganisms were respectively prepared, added into a 100L fermenter with a liquid volume of 70%, sterilized, and cooled to room temperature. Take one preserved EM bacteria, Bacillus licheniformis, and Bifidobacterium respectively, and inoculate them into the following seed culture medium in parts, and cultivate them ...

Embodiment 3

[0051] A preparation method of a composite micro-ecological water quality regulator, comprising the steps of:

[0052] (1) Preparation of preserved bacteria liquid

[0053] First prepare the pure strains of EM bacteria, Bacillus licheniformis and Bifidobacterium, and dilute the bacteria content to 1×10 10 CFU / mL, 50mL per tube is distributed to each preservation tube, 40% glycerin is added as a protective agent, and it is stored at -20°C. The whole process requires aseptic operation.

[0054] (2) Preparation of fermented seed liquid

[0055] A 100L fermentation system was used to prepare the fermented seed liquid, and the seed culture medium of three kinds of microorganisms were respectively prepared, added into a 100L fermenter with a liquid volume of 70%, sterilized, and cooled to room temperature. Each one of the preserved EM bacteria, Bacillus licheniformis and Bifidobacterium is inoculated into the seed culture medium, cultivated to the logarithmic phase according to th...

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Abstract

The invention relates to a composite micro-ecological water quality regulator and a preparation method thereof. The regulator is prepared from, by weight, 1-1.2% of vitamin A, 1-1.2% of vitamin B2, 0.5-0.8% of vitamin B6, 10-15% of vitamin C, 4-8% of taurine and 73.8-83.5% of glucose. In the regulator, the content concentration of mixed bacteria is 107-109 CFU / g. During preparation, firstly, EM, bacillus licheniformis and bifidobacterium are prepared into a fermentation seed solution, then fermentation liquor is prepared, and lastly the mixed fermentation liquor, vitamin A, vitamin B2, vitamin B6, vitamin C, taurine and glucose are subjected to mixing granulation, and the regulator is prepared. The preparation method is simple, the steps are easy to operate, the prepared regulator is suitable for aquaculture of all marine and freshwater varieties such as finless eels, loaches, fishes, shrimps, crabs and sea cucumbers, aquaculture water can be restored quickly, the physique and anti-stress ability of bred animals are enhanced, the survival rate of fry is increased, digestion is promoted, absorption is guaranteed, the yield of the bred animals is raised, and the quality of the bred animals is improved.

Description

technical field [0001] The invention relates to a composite micro-ecological water quality regulator and a preparation method thereof, belonging to the fields of aquaculture technology and microorganism technology. Background technique [0002] China is a big aquaculture country, and the breeding and production of aquatic animals accounts for more than 70% of the global total, occupying an important position in my country's agricultural production. At present, the annual output of aquaculture in my country exceeds 45 million tons, and the annual growth rate remains at about 7% all the year round, achieving continuous and rapid growth for many years. With the continuous improvement of my country's economic level and people's needs for dietary diversification, the demand for aquatic products continues to increase. In this situation, farmers use a large amount of feed, chemical additives, chemical drugs and even fertilizers in order to pursue high yields, which brings a series...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12N1/16C02F103/20C02F101/30C02F101/16C12R1/10C12R1/01
CPCC02F3/34C02F3/348C02F2101/101C02F2101/166C02F2101/30C02F2103/20C02F2209/06C02F2305/06C12N1/16C12N1/20
Inventor 杨东辉伦峰李永胜郭旭升雷宇杰吴坤杰黄荣静刘俊张六六夏森玉
Owner XINYANG AGRI & FORESTRY UNIV
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