Predication model of Salmonella typhimurium on surface of strawberries under warm rinse condition, and establishing method of model

A technology of Salmonella and prediction model, which is applied to the prediction model of Salmonella Typhimurium on strawberry surface under warm water washing conditions and its establishment field, which can solve the problems of residents' health risks, bacterial contamination, bacterial growth, etc.

Active Publication Date: 2016-12-07
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although my country still lacks relevant reports on the pollution rate and level of Salmonella typhimurium on the surface of strawberries, fertilization, irrigation and other processes may cause bacterial contamination, and the warmer temperature in the ripening season of strawberries in spring can cause the growth of bacteria on the fruit surface, which has serious consequences for the health of residents. risk
At present, there are few domestic quantitative risk assessment studies on Salmonella on strawberry surfaces, and there is also a lack of healthy consumption guidance for consumers at home based on scientific research results

Method used

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  • Predication model of Salmonella typhimurium on surface of strawberries under warm rinse condition, and establishing method of model
  • Predication model of Salmonella typhimurium on surface of strawberries under warm rinse condition, and establishing method of model
  • Predication model of Salmonella typhimurium on surface of strawberries under warm rinse condition, and establishing method of model

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Embodiment Construction

[0046] The present invention will be described in detail below in conjunction with the drawings:

[0047] 1Materials and methods

[0048] (1) Strain and bacterial culture

[0049] In this experiment, Salmonella Typhimurium (Salmonella Typhimurium), numbered ATCC14028, was provided by the Agricultural Products Quality Standards Institute of Zhejiang Academy of Agricultural Sciences.

[0050] Strains are stored in a 1ml freezing tube, stored in a refrigerator at -80°C, taken out before use, and after thawing, 1ml of bacterial liquid is sucked and cultured in 300ml of NB broth medium (purchased from Luqiao Technology Co., Ltd.), and cultured at 37°C with shaking for 12h , Obtain a bacterial culture solution with a bacterial concentration of 108 CFU / ml for use.

[0051] (2) Sample preparation and inoculation

[0052] Fresh strawberries were purchased from nearby supermarkets, and the stalks were removed and weighed one by one. Strawberries of uniform size (7±0.3g / piece) were selected for ex...

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Abstract

The invention provides a predication model of Salmonella typhimurium on the surface of strawberries under a warm rinse condition, represented by formula represented by Y(t) = logNt - logN0 = (0.0194 - 0.0005T) * t<1.015>, and an establishing method of the model. Model verification confirms that the model can accurately, objectively and quantitatively reflect the change of Salmonella typhimurium on the surface of the strawberries under the warm rinse condition, provides data bases for researches of strawberry risk assessment models, and is in favor of realizing quality control and risk early warning and assessment of the strawberries.

Description

Technical field [0001] The invention relates to a prediction model of Salmonella typhimurium on strawberry surface under warm water cleaning conditions and a method for establishing the same. Background technique [0002] Salmonella Typhimurium (Salmonella Typhimurium) is a gram-negative enterobacteria and belongs to Salmonella group B. It can cause various host animal intestinal diseases. It can enter the human body through contaminated drinking water and food, causing diarrhea, vomiting, enteritis and other diseases It can cause death in severe cases. Its pathogenic mechanism is that after Salmonella typhimurium enters the human intestine, it can cause intestinal edema, congestion, and tissue inflammation by invading small intestinal epithelial cells and under the action of enterotoxin and endotoxin. Salmonella is ubiquitous in animal feces and spread through irrigation water and fertilization in agricultural facilities. In September 2015, the U.S. emergency recall of imported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12R1/42
CPCC12Q1/10
Inventor 汪雯杨桂玲王强宋雯周育赵慧宇蔡铮
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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