Predication model of Salmonella typhimurium on surface of strawberries under warm rinse condition, and establishing method of model
A technology of Salmonella and prediction model, which is applied to the prediction model of Salmonella Typhimurium on strawberry surface under warm water washing conditions and its establishment field, which can solve the problems of residents' health risks, bacterial contamination, bacterial growth, etc.
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[0046] The present invention will be described in detail below in conjunction with the drawings:
[0047] 1Materials and methods
[0048] (1) Strain and bacterial culture
[0049] In this experiment, Salmonella Typhimurium (Salmonella Typhimurium), numbered ATCC14028, was provided by the Agricultural Products Quality Standards Institute of Zhejiang Academy of Agricultural Sciences.
[0050] Strains are stored in a 1ml freezing tube, stored in a refrigerator at -80°C, taken out before use, and after thawing, 1ml of bacterial liquid is sucked and cultured in 300ml of NB broth medium (purchased from Luqiao Technology Co., Ltd.), and cultured at 37°C with shaking for 12h , Obtain a bacterial culture solution with a bacterial concentration of 108 CFU / ml for use.
[0051] (2) Sample preparation and inoculation
[0052] Fresh strawberries were purchased from nearby supermarkets, and the stalks were removed and weighed one by one. Strawberries of uniform size (7±0.3g / piece) were selected for ex...
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