Detection kit of non-esterified fatty acids and preparation method thereof

A detection kit and free fatty acid technology, applied in the field of medical detection, can solve the problems of affecting the detection accuracy, easy precipitation, unstable enzyme activity, etc.

Active Publication Date: 2016-12-07
HONGKUI BIOLOGICAL CHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the enzyme activity in the free fatty acid determination kit is unstable and easy to precipitate, which affects the detection accuracy, the application discloses a free fatty acid detection kit with stable enzyme activity, low precipitation and high detection accuracy in the kit.

Method used

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  • Detection kit of non-esterified fatty acids and preparation method thereof
  • Detection kit of non-esterified fatty acids and preparation method thereof
  • Detection kit of non-esterified fatty acids and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A free fatty acid detection kit, containing reagent 1 and reagent 2,

[0039] The composition of reagent 1 is as follows:

[0040] Sodium dihydrogen phosphate 50mmol / L,

[0041] Coenzyme A 0.05mmol / l,

[0042] Adenosine triphosphate 3mmol / L,

[0043] Acyl-CoA synthetase 0.4KU / L,

[0044] MgCl 2 2mmol / l,

[0045] Trinder substrate 0.5 g / l,

[0046] Adjust the pH to 7.2;

[0047] The composition of reagent 2 is as follows:

[0048] Sodium dihydrogen phosphate 60mmol / L,

[0049] Flavin adenine dinucleotide 2mmol / l,

[0050] 4 aminoantipyrine 10mmol / L,

[0051] Acyl-CoA oxidase 40KU / L,

[0052] Peroxidase 40KU / L,

[0053] Alkyl glycoside APG0810 1.5g / L,

[0054] Mannitol 0.5g / L,

[0055] EDTA-2Na 1g / L,

[0056] Lauryl polyoxyethylene ether 2g / L,

[0057] Adjust the pH to 7.2.

[0058] The volume ratio of reagent 1 and reagent 2 is 4:1.

[0059] Preparation of reagent 1: Take water, add sodium dihydrogen phosphate, fully dissolve, adjust pH to 7.2 with NaO...

Embodiment 2

[0062] A free fatty acid detection kit, containing reagent 1 and reagent 2,

[0063] The composition of reagent 1 is as follows:

[0064] Sodium dihydrogen phosphate 50mmol / L,

[0065] Coenzyme A 0.05mmol / l,

[0066] Adenosine triphosphate 3mmol / L,

[0067] Acyl-CoA synthetase 0.4KU / L,

[0068] MgCl 2 2mmol / l,

[0069] Trinder substrate 0.5 g / l,

[0070] Adjust the pH to 7.2;

[0071] The composition of reagent 2 is as follows:

[0072] Sodium dihydrogen phosphate 60mmol / L,

[0073] Flavin adenine dinucleotide 2mmol / l,

[0074] 4 aminoantipyrine 10mmol / L,

[0075] Acyl-CoA oxidase 40KU / L,

[0076] Peroxidase 40KU / L,

[0077] Alkyl glycoside APG0810 1g / L,

[0078] Mannitol 0.5g / L,

[0079] EDTA-2Na 1g / L,

[0080] Lauryl polyoxyethylene ether 3g / L,

[0081] Adjust the pH to 7.2.

[0082] The volume ratio of reagent 1 and reagent 2 is 4:1.

[0083] Preparation of reagent 1: Take water, add sodium dihydrogen phosphate, fully dissolve, adjust pH to 7.2 with NaOH, a...

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Abstract

The invention relates to the field of medical detection technology, and especially relates to a detection kit of non-esterified fatty acids. The kit comprises a reagent 1 and a reagent; the reagent 1 comprises sodium dihydrogen phosphate, coenzyme A, adenosine triphosphate, acyl coenzyme A synthetase, MgCl2, and a Trinder substrate, wherein the pH value is adjusted to 7.2; the reagent 2 comprises sodium dihydrogen phosphate, flavin adenine dinucleotide, 4-aminoantipyrine, acyl coenzyme A synthetase, peroxidase, alkyl glucoside APG0810, mannitol, EDTA-2Na, and polyoxyethylene dodecyl ether, where the pH value is adjusted to 7.2. Surfactants in the reagents are selected, so that activity of various enzymes is stable and are not easy to precipitate, shelf-life is prolonged, enterprise risk cost is reduced, and enterprise benefits are improved.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a free fatty acid detection kit, and also to a preparation method of the free fatty acid detection kit. Background technique [0002] Free fatty acids, namely non-esterfied fatty acid (NEFA), are intermediate products of fat metabolism in the human body, and are also substrates of cell membrane lipid structures and donors of intracellular signaling molecules such as prostaglandins. When glycogen, the energy source for muscle activity, is exhausted, adipose tissue will decompose neutral fat into free fatty acids for energy use. Although free fatty acids only account for a small part of body fat, they meet a large part of energy demand, so they are important indicators for monitoring body fat metabolism and glucose metabolism. Free fatty acids can not only reflect the body's fat metabolism and blood lipid levels, evaluate blood sugar and assist in the diagnosis of diabet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12Q1/28C12Q1/48
CPCC12Q1/26C12Q1/28C12Q1/48
Inventor 王保全靳峰
Owner HONGKUI BIOLOGICAL CHINA CO LTD
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