Black phosphorus nanosheet and antitumor compound containing composite material and preparation method and application thereof

A composite material and nanosheet technology, applied in the field of anti-tumor materials, can solve the problems of low loading rate, poor biocompatibility, single function, etc., and achieve the effects of high loading rate, small side effects, and easy degradation.

Inactive Publication Date: 2017-01-04
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of low loading rate, single function, and poor biocompatibility of existing nano-drug-loading materials, the present invention provides a composite material prepared

Method used

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  • Black phosphorus nanosheet and antitumor compound containing composite material and preparation method and application thereof
  • Black phosphorus nanosheet and antitumor compound containing composite material and preparation method and application thereof
  • Black phosphorus nanosheet and antitumor compound containing composite material and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0055] The preparation of step (1) BP nanosheet:

[0056] BP nanosheets were prepared by exfoliating black phosphorus powder samples by liquid exfoliation method. The specific method is: Weigh 20 mg of black phosphorus powder and disperse it in 50 mL of saturated NaOH N-methylpyrrolidone (NMP) solution, sonicate in an ice bath for 10 hours (20-40 kHz, 150-300 W) to obtain a dark brown suspension, and centrifuge at a low speed (4000r / min) for 8min, remove the unstripped large pieces of black phosphorus, collect the supernatant to obtain a brown transparent liquid, which is black phosphorus nanosheets. Store in a refrigerator at 4°C, and centrifuge at high speed (12000r / min) for 10 minutes before use to obtain a precipitate, which is dispersed in the desired solution by ultrasonication.

[0057] Depend on figure 1 It can be seen that the BP nanosheets prepared in step 1 are uniform nanosheets with a thickness of about 5.5 nm and a diameter of 200 nm.

[0058] Step (2) BP nano...

Embodiment 2

[0067] The preparation of step (1) BP nanosheet is the same as step 1 of embodiment 1

[0068] Step (2) BP nanosheets loaded with anticancer drugs (curcumin (CUR))

[0069]First use 0.1M NaOH to adjust the pure water to pH = 7.4. Disperse the BP nanosheet precipitation obtained above in an aqueous solution with a pH of 7.4 to obtain an aqueous solution of BP nanosheets with a concentration of 50 μg / mL. 7.4 to obtain a CUR aqueous solution with a concentration of 2.5 mg / mL in the water. Take 3mL of BP nanosheet aqueous solution (50μg / mL), and add 2mL of 2.5mg / mL CUR aqueous solution to it, then stir vigorously at room temperature in the dark for 12h, collect the precipitate by high-speed centrifugation (12000r / min), wash three times and disperse to obtain the loaded CUR BP nanosheet solution. Change the concentration of CUR so that the final concentration of the mixed solution is 125, 250, 500, 1000 μg / mL, and measure the loading capacity of CUR at the corresponding concentra...

Embodiment 3

[0073] The preparation of step (1) BP nanosheet is the same as step 1 of embodiment 1

[0074] Step (2) BP nanosheets loaded with anti-tumor drugs (changing the concentration of doxorubicin (DOX)):

[0075] First use 0.1M NaOH to adjust the pure water to pH=7.4. Disperse the BP nanosheet precipitation obtained above in an aqueous solution with a pH of 7.4 to obtain an aqueous solution of BP nanosheets with a concentration of 50 μg / mL, and simultaneously dissolve DOX in a solution with a pH of 7.4 The concentrations in the water are 0.625mg / mL, 1.25mg / mL, and 2.5mg / mL DOX aqueous solution respectively. Take 3 mL of BP nanosheet aqueous solution (50 μg / mL), and add 2 mL of DOX aqueous solution of different concentrations to it, then vigorously stir for 12 h at room temperature in the dark, and collect the precipitate by high-speed centrifugation (12000 r / min), wash three times and disperse to obtain DOX-loaded BP nanosheet solution. The loading capacity of DOX at the correspon...

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Abstract

The invention discloses a black phosphorus nanosheet and antitumor compound containing composite material and a preparation method and application thereof. The composite material is obtained by compounding of black phosphorus nanosheets and an antitumor compound with a primary amino group and/or a phenolic hydroxyl group under the condition that pH is 7.2-7.6. By stirring of the antitumor compound with the primary amino group and the phenolic hydroxyl group under the pH condition, antitumor compound molecules can be electrostatically adsorbed and accumulated on surfaces of the black phosphorus nanosheets, and problems of low load rate of the antitumor compound, antitumor property loss of each component of the composite material and the like can be solved effectively. The composite material is high in antitumor drug load rate and biocompatibility and capable of multi-functionally and synergistically improving antitumor efficiency. In addition, the composite material is low in side effect and easy to degrade.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical materials; in particular, it relates to a novel multifunctional anti-tumor material prepared from black phosphorus nanosheets / anti-tumor compounds through simple electrostatic adsorption assembly and compounding, as well as its preparation and application. Background technique [0002] As a novel layered material, black phosphorus has received extensive attention in the past few years. Because its structure is similar to graphene and can be exfoliated into a single-layer structure, it is called "phosphorene". Unlike graphene, the thickness of the nanosheets of black phosphorus can affect its energy band gap, which is 0.3 eV in bulk black phosphorus and increases to 2.0 eV in monolayer black phosphorus. In addition, its absorption band spans the ultraviolet and the entire visible light region. It is because of this unique photoelectric property that it has been widely used in gas sensors, ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/704A61K31/12A61P35/00
Inventor 刘又年欧阳江陈万松邓留曾科刘洪
Owner CENT SOUTH UNIV
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