Detection method of compound lonicera granules

A technology of compound honeysuckle and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2017-01-04
HEBEI GOGIN PHARMA
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  • Detection method of compound lonicera granules
  • Detection method of compound lonicera granules
  • Detection method of compound lonicera granules

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[0164] The present invention will be further described below in conjunction with the examples, but it should not be interpreted as a limitation of the present invention. The protection scope of the present invention is based on the contents recorded in the claims. protection scope of the present invention.

[0165]The detection method of compound honeysuckle granules, using high performance liquid chromatography to detect neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, forsythiaside A, forsythin, pinoresin, baicalin, and Chinese scutellaria in compound honeysuckle granules Simultaneous detection of the element; including the following process steps:

[0166] A. Preparation of reference solution

[0167] Take chlorogenic acid, forsythin, and baicalin reference substances, and add 50% methanol by mass percentage to make solutions containing 50 μg of chlorogenic acid, 20 μg of forsythin, and 0.25 mg of baicalin per 1 ml;

[0168] B. Preparation of the test soluti...

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Abstract

The invention belongs to analysis and detection of drugs, and particularly relates to a method for simultaneously detecting various effective components in compound lonicera granules with high performance liquid chromatography. The method is used for simultaneously detecting neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, forsythiaside A, forsythin, pinoresinol, baicalin and wogonin in the compound lonicera granules with the high performance liquid chromatography and comprises steps as follows: preparation of a reference substance solution, preparation of a solution of a testing product, determination, result calculation and the like. The method effectively solves the problems that quality evaluation of the compound lonicera granules is mainly about single-component content determination and the like in the prior art, and has the advantages of comprehensively characterizing active pharmaceutical ingredients of the compound lonicera granules and being high in repeatability and good in precision and the like.

Description

technical field [0001] The invention belongs to the analysis and detection of medicines, in particular to a detection method for simultaneously detecting multiple active ingredients in compound honeysuckle granules by using high performance liquid chromatography. Background technique [0002] Compound Chinese medicine is the main form of TCM clinical medication, and it is the characteristic and essence of TCM. Traditional Chinese medicine is a complex system composed of multiple components and factors. The diversity and complexity of its chemical components are the material basis for its curative effect. Establish a modern quality control system in line with the characteristics of Chinese medicine, overcome the problems of quality analysis and evaluation of Chinese medicine, and improve The existing quality control methods of traditional Chinese medicine have become the subject of active research. [0003] The establishment of a quality control system for traditional Chines...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张四岩赵国强白新涛王建允
Owner HEBEI GOGIN PHARMA
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