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Gansu Longnan Zanthoxylum Bungeagum Maxim extract component and preparation and detection methods thereof

A Zanthoxylum bungeanum extract and extract technology, applied in the field of Zanthoxylum bungeanum extract components and its preparation in Longnan, Gansu, can solve the problems of unsatisfactory anesthesia

Active Publication Date: 2017-01-11
GANSU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The inventor of the present invention, as a scientific researcher of Gansu University of Traditional Chinese Medicine, an important unit of Dahongpao pepper research in Gansu Longnan, has conducted research on Gansu Longnan pepper for many years. The extract has a certain anesthetic effect, but researchers have found that only the Longnan Zanthoxylum bungeanum extract prepared under a specific extraction and purification method has a good anesthetic effect, while the conventional water extraction and alcohol extraction of the Longnan Zanthoxylum bungeanum extract Anesthesia is not ideal

Method used

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  • Gansu Longnan Zanthoxylum Bungeagum Maxim extract component and preparation and detection methods thereof
  • Gansu Longnan Zanthoxylum Bungeagum Maxim extract component and preparation and detection methods thereof
  • Gansu Longnan Zanthoxylum Bungeagum Maxim extract component and preparation and detection methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0323] Prescription: pepper 20Kg

[0324] Preparation:

[0325] (1) Take 20Kg of Zanthoxylum bungeanum powder (40 mesh), dry it in an oven at 45°C for 4 hours, add 20 times the amount of 60% acetone aqueous solution, and extract 3 times under reflux in a water bath at 65°C, each time for 75min, combine the extracts, and recover Acetone to obtain the total extract of Zanthoxylum bungeanum;

[0326] (2) Stir and dissolve the obtained Zanthoxylum bungeanum extract with chloroform:methanol=50:5 mixed solution under 45°C water bath conditions, filter, and carry out column chromatography separation after the filtrate is concentrated under reduced pressure in a 45°C water bath;

[0327] (3) Choose D101B type macroporous adsorption resin for column chromatography separation, resin column diameter to height ratio is 1:8, sample volume is 7BV, washed with water for 5BV to remove impurities, ethyl acetate: 0.2mol / L sodium dihydrogen phosphate solution =100:5 eluent 8BV elution, flow ra...

Embodiment 2

[0343] Prescription: pepper 20Kg

[0344] Preparation:

[0345] (1) Take 20Kg of Zanthoxylum bungeanum powder (40 mesh), dry it in an oven at 45°C for 4 hours, add 20 times the amount of 60% acetone aqueous solution, and extract 3 times under reflux in a water bath at 65°C, each time for 75min, combine the extracts, and recover Acetone to obtain the total extract of Zanthoxylum bungeanum;

[0346] (2) Stir and dissolve the obtained Zanthoxylum bungeanum extract with chloroform:methanol=50:5 mixed solution under 45°C water bath conditions, filter, and carry out column chromatography separation after the filtrate is concentrated under reduced pressure in a 45°C water bath;

[0347] (3) Choose D101B type macroporous adsorption resin for column chromatography separation, resin column diameter to height ratio is 1:8, sample volume is 7BV, washed with water for 5BV to remove impurities, ethyl acetate: 0.2mol / L sodium dihydrogen phosphate solution =100:5 eluent 8BV elution, flow ra...

Embodiment 3

[0363] Prescription: pepper 20Kg

[0364] Preparation:

[0365] (1) Take 20Kg of Zanthoxylum bungeanum powder (40 mesh), dry it in an oven at 45°C for 4 hours, add 20 times the amount of 60% acetone aqueous solution, and extract 3 times under reflux in a water bath at 65°C, each time for 75min, combine the extracts, and recover Acetone to obtain the total extract of Zanthoxylum bungeanum;

[0366] (2) Stir and dissolve the obtained Zanthoxylum bungeanum extract with chloroform:methanol=50:5 mixed solution under 45°C water bath conditions, filter, and carry out column chromatography separation after the filtrate is concentrated under reduced pressure in a 45°C water bath;

[0367] (3) Choose D101B type macroporous adsorption resin for column chromatography separation, resin column diameter to height ratio is 1:8, sample volume is 7BV, washed with water for 5BV to remove impurities, ethyl acetate: 0.2mol / L sodium dihydrogen phosphate solution =100:5 eluent 8BV elution, flow ra...

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Abstract

The invention belongs to the field of plant extracts and relates to a Gansu Longnan Zanthoxylum Bungeagum Maxim extract component with an anesthetic action and preparation and detection methods thereof. The extract is prepared by adopting the following methods: (1) crushing pericarpium zanthoxyli, baking the pericarpium zanthoxyli powder in a baking oven, then, adding a 50%-60% acetone aqueous solution into the baked pericarpium zanthoxyli powder, carrying out water-bath reflux extraction, recovering acetone from the extract, thereby obtaining total extract concretes of the pericarpium zanthoxyli; (2) dissolving the obtained total extract concretes of the pericarpium zanthoxyli in a mixed solution of chloroform and methanol, which are in the ratio of 50 to 5, with stirring under water-bath conditions, carrying out filtration, carrying out depressurized concentration on the filtrate in water bath, and then, carrying out column-chromatography separation; (3) carrying out column-chromatography separation by using D101B-type macroporous adsorption resin, carrying out water-washing on 4BV to 6BV so as to remove impurities, carrying out eluting on 7BV to 9BV with an eluant, of which the ratio of ethyl acetate to acetone is 100 to 5, at the flow velocity of 2BV / h to 4BV / h, collecting eluate, concentrating the eluate, and carrying out drying, thereby obtaining the extract, wherein the ratio of diameter to height of a resin column is 1 to (7 to 9), and the sample volume is 6BV to 8BV.

Description

[0001] Technical field: [0002] The invention belongs to the field of plant extracts, and in particular relates to a Gansu Longnan prickly ash extract component with anesthetic effect and a preparation method thereof. [0003] technical background: [0004] Zanthoxyli (Pericarpium Zanthoxyli) is the dried and mature peel of Rutaceae green pepper (Zanthoxylum schinifoliumsieb.et Zucc.) or Zanthoxylum bungeanum Maxim. medicine. It is stipulated in the "Pharmacopoeia of the People's Republic of China" that pepper is a traditional Chinese medicine, which has the effects of "warming the middle and relieving pain, killing insects and relieving itching". [0005] Modern research results show that the chemical components of plants of the genus Zanthoxylum at home and abroad mainly include alkaloids, amides, lignans, coumarins, volatile oils and fatty acids, etc. , anti-inflammation, local anesthesia, antibacterial and scabies-killing effects, anti-tumor, etc. have strong pharmacolog...

Claims

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Application Information

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IPC IPC(8): A61K36/758A61P23/00A61P29/00A61P19/02A61P9/00A61P35/00G01N30/02
CPCA61K36/758A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/55G01N30/02G01N2030/025G01N2030/027
Inventor 李越峰严兴科吴平安杜丽东刘安国金辉曹瑞牛江涛边甜甜司昕蕾
Owner GANSU UNIV OF CHINESE MEDICINE
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