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A kind of high-salt organic industrial wastewater treatment microbial agent protective agent and its application

A technology of industrial wastewater and protective agent, which is applied in the field of microbial preservation, can solve the problems of short preservation period, easy-to-contaminate bacteria performance, cumbersome methods, etc., and achieve good protection effect, reduce bacterial pollution, and high activity.

Active Publication Date: 2019-12-06
江苏中宜金大环保产业技术研究院有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that the existing microbial agent preservation methods are cumbersome, require low temperature or short shelf life, are easy to infect bacteria, and have rapid performance degradation, one of the purposes of the present invention is to provide a microbial agent for the treatment of high-salt organic industrial wastewater for protection. agent, which is composed of antioxidants, buffers, nutrients and inhibitors, so that the microbial agent can be preserved at room temperature, and the preserved microbial agent is not easy to be infected with bacteria, and has stable performance

Method used

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  • A kind of high-salt organic industrial wastewater treatment microbial agent protective agent and its application
  • A kind of high-salt organic industrial wastewater treatment microbial agent protective agent and its application
  • A kind of high-salt organic industrial wastewater treatment microbial agent protective agent and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preservation of Microbial Agents at Room Temperature for Penicillin Wastewater Treatment

[0028] (1) Penicillin wastewater treatment microbial agent survival rate

[0029] Inoculate the penicillin wastewater treatment microbial bacterial agent seed liquid into 1 liter of fermentation culture liquid in a ratio of 2% (V / V) (the fermentation culture liquid formula is: peptone 10g, beef extract 6g, sodium sulfate 40g, add normal saline to constant volume to 1000mL, adjust the pH value to 6.5), and shake and cultivate in the dark at a temperature of 30°C and a shaking speed of 150r / min until the stationary phase. Measure the number of viable bacteria in the fermentation broth in the stationary phase by plate counting method, and dilute the fermentation broth so that the number of viable bacteria is 10 10 cfu / ml. Then, after the diluted fermented liquid was centrifuged at 5000rpm for 20 minutes, the remaining fermented liquid was discarded, leaving the strains, and finally...

Embodiment 2

[0045] Preservation of Microbial Agents for Phenol Wastewater Treatment at Room Temperature

[0046] Inoculate the phenol wastewater treatment microbial bacterial agent seed liquid into 1 liter of fermented culture liquid in the ratio of 1% (V / V) (the fermented culture liquid formula is: peptone 10g, beef extract 6g, sodium chloride 10g, phenol 0.1g, Add physiological saline to make the volume to 1000mL, adjust the pH value to 7.3), and culture in the dark at a temperature of 35°C and a shaking speed of 120r / min until a stable period. Measure the number of viable bacteria in the fermentation broth in the stationary phase by plate counting method, and dilute the fermentation broth so that the number of viable bacteria is 10 10 cfu / ml. Then, after the diluted fermentation broth was centrifuged at 8000rpm for 10 minutes, the remaining fermentation broth was discarded, leaving the bacterial classification, and finally adding 1L formula in the bacterial classification was No. 5 an...

Embodiment 3

[0051] Preservation of Microbial Agents at Room Temperature for Treatment of Phthalic Acid Wastewater

[0052] According to the ratio of 2% (V / V), inoculate the phthalic acid wastewater treatment microbial bacterial agent seed liquid into 1 liter of fermentation culture liquid (the fermentation culture liquid formula is: peptone 10g, beef extract 6g, sodium chloride 10g, benzene two Add 0.3 g of formic acid, add physiological saline to make the volume to 1000 mL, adjust the pH value to 7.3), and culture at a temperature of 35 ° C and a shaking speed of 120 r / min in the dark and shake until the stable phase. Measure the number of viable bacteria in the fermentation broth in the stationary phase by plate counting method, and dilute the fermentation broth so that the number of viable bacteria is 10 10 cfu / ml. Then, after the diluted fermentation broth was centrifuged at 5000rpm for 30 minutes, the remaining fermentation broth was discarded, leaving the bacterial classification, ...

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Abstract

The invention discloses a high-salt organic industrial wastewater treatment microbial agent protective agent and application thereof, belonging to the technical field of microbial preservation. The high-salt organic industrial wastewater treatment microbial agent protective agent solves the problems of method complication, requirements on low temperature, short preservation validity period, easy bacterial contamination and quick performance degradation in a traditional microbial agent preservation method. The protective agent comprises an antioxidant, a buffering agent, a nutritional agent, an inhibitor and the like, wherein the antioxidant comprises ascorbic acid and citric acid; the buffering agent comprises a phosphate buffer and glycerin; the nutritional agent comprises organic industrial wastewater specific pollutants (such as penicillin and phenol) and a composite nutrient solution; and the inhibitor comprises sodium sulfate or sodium chloride or sodium sulfate and sodium chloride. The high-salt organic industrial wastewater treatment microbial agent protective agent is low in cost and convenient to use and is capable of effectively maintaining the properties of a microbial agent, prolonging the service life of the microbial agent and reducing the preservation cost of the microbial agent.

Description

technical field [0001] The invention belongs to the technical field of microbial preservation, and more specifically relates to a protective agent for microorganism bacteria agents for high-salt organic industrial wastewater treatment and an application thereof. Background technique [0002] At present, my country has promulgated and implemented the "Water Pollution Prevention and Control Action Plan" in response to problems such as poor water environment quality and many environmental hazards in some areas. Paying close attention to industrial pollution prevention and control is an important part of it. Organic industrial wastewater is one of the more typical types of industrial wastewater. The characteristics of its wastewater mainly include high organic pollutants, high salinity, and refractory biodegradation, which makes it difficult to achieve efficient treatment by conventional biological treatment methods. In response to this problem, studies have shown that by adding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 张徐祥陶文达叶林任洪强
Owner 江苏中宜金大环保产业技术研究院有限公司
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