Method for producing anti-ApoA1 antiserum through goats

A goat and serum technology, applied in the direction of serum immunoglobulin, anti-animal/human immunoglobulin, etc., can solve the problems of unsatisfactory use requirements, poor antibody stability, low yield and titer, etc., and achieve high Significant application value and benefit, economic value and social value, cost reduction effect

Inactive Publication Date: 2017-02-01
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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Problems solved by technology

It is an important raw material for biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use.
[0003] Chinese patent CN103467600A (method for producing anti-ApoA1 polyantiserum by using Hu sheep) uses Hu sheep to obtain anti-APOA1 polyantiserum with antibody titer up to 1:16, but there are still insufficient antibody titers and poor antibody stability , the defect of low sensitivity

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment

[0023] test subject:

[0024] Choose 50 goat rams, choose healthy sheep with a weight of about 35 kg, and put ear tags on all of them before immunization. Grass is supplied every day, and concentrate, vinegar grains, bran and vitamins are added in an appropriate amount.

[0025] experimental method:

[0026] A method utilizing goats to produce anti-ApoA1 multiple antisera, comprising the following steps:

[0027] (1) Preparation of immunogenic emulsion: Mix and emulsify 0.1ml of serum containing 10mg of ApoA1 antigen, 1.9ml of sterile PBS, and 2ml of Freund's adjuvant to obtain immunogenic emulsion for the first dose of immunization; the second dose of ApoA1 The amount of antigen is increased to 20mg, the third injection is increased to 30mg, the fourth injection is increased to 40mg, the fifth injection is increased to 50mg, the sixth injection is increased to 60mg, the seventh injection is increased to 70mg, and the rest are the same;

[0028] Among them, the ApoA1 antige...

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PUM

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Abstract

The invention discloses a method for producing anti-ApoA1 antiserum through goats. The method includes the steps that goats with the higher immune response capacity and the low serum fat content are selected as raw materials, an ApoA1 antigen is subcutaneously injected into the outside of the goat groin to carry out multiple times of booster immunization, seven times of immune injection are carried out, and the quantities of the injected ApoA1 antigen are gradually increased; responses of T cells and B cells can be continuously activated, effective antibodies are better generated, and the valence of the generated efficient goat anti-ApoA1 antibodies can be 1:32 or above. The valence of the antibodies is greatly improved, the stability and the sensitivity are increased, raw materials are provided for biological reagent plants, convenience is brought to medical diagnoses, and goats generate high application value and benefits accordingly; meanwhile, the cost of reagent factories is reduced by 50%, and the economic value and the social value are remarkable.

Description

technical field [0001] The invention relates to the field of antigen preparation methods, in particular to a method for producing anti-ApoA1 polyantiserum by using goats. Background technique [0002] Apolipoprotein A1 (ApoA1) is the largest component of the apoA ApoA1 family. ApoA1 is a single polypeptide chain consisting of 243 amino acid residues with a molecular weight of 28300D. It is the main apolipoprotein of high-density lipoprotein (HDL). It is an important raw material of biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use. [0003] Chinese patent CN103467600A (method for producing anti-ApoA1 polyantiserum using Hu sheep) used Hu sheep to obtain anti-APOA1 polyantiserum with antibody titer up to 1:16, but there are still insufficient antibody titers and poor antibody stability , The defect of low sensitivity. [0004] Therefore, a new...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/06
CPCC07K16/18C07K16/06
Inventor 徐志伟
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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