Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method

A technology for prostate cancer and tumor cells, which is applied in the field of separation and cultivation of circulating tumor cells of prostate cancer in mice, can solve problems such as separation and cultivation methods of circulating tumor cells of prostate cancer that have not yet been seen, and achieve simple and easy-to-operate separation and cultivation methods, Strong metastasizing and invasive ability, uniform quality effect

Inactive Publication Date: 2017-02-01
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0003] So far, there is no report on the isolation and culture of prostate cancer circulating tumor cells

Method used

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  • Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method
  • Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method
  • Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0025] The present invention proposes a method for isolating and culturing circulating tumor cells of prostate cancer in mice, and by this method for the first time, the circulating tumor cells MPC2013 of orthotopic prostate cancer NOD / SCID mice were obtained, which has been preserved in the Chinese Type Culture Collection Center (China, Wuhan, Wuhan University), the deposit date is December 22, 2015, and the deposit number is CCTCC NO: C201601.

[0026] (1) Relevant solutions involved in this embodiment

[0027] (1) 1000mL 10× red blood cell lysate storage solution:

[0028] NH 4 Cl 88.26g (Sigma A9434), KHCO 3 Add 10.01g (Sigma P9144) and 0.37g EDTA (SigmaE5134) into distilled water, adjust the pH to 7.2 with 1M HCl, and store at room temperature. Diluted 10 times for use.

[0029] (2) 1640 medium (GIBCO, USA)

[0030] (3) 1640 culture medium...

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Abstract

The invention discloses a mouse prostate cancer circulating tumor cell line and a prostate cancer circulating tumor cell isolating and culturing method. The preservation number of the circulating tumor cell line is CCTCC C201601. The isolating and culturing method comprises the following steps: establishing an in-situ prostate cancer animal model, taking blood, lysing red blood cells, and conducting isolating and culturing and passage. Obtained circulating tumor cells are uniform in quality; based upon immunofluorescence as well as scratch and membrane-penetrating experiments, the obtained circulating tumor cells conform to the properties of the circulating tumor cells and the circulating tumor cells are relatively strong in transfer and invasion capacities; and the circulating tumor cells can serve as a good experimental tool for researching a prostate cancer transfer mechanism in a laboratory and can also provide a cell research model for preventing prostate cancer transfer in the clinical field. The circulating tumor cell isolating and culturing method provided by the invention is simple and easy to operate, and the isolated cells can undergo stable passage, so as to facilitate such experiments as researching the properties of the circulating tumor cells and the like in the laboratory. The circulating tumor cell line and the circulating tumor cell isolating and culturing method provided by the invention can have a broad application scope in the field of researching tumor transfer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for separating and culturing mouse prostate cancer circulating tumor cells. Background technique [0002] Patients with advanced prostate cancer are often accompanied by tumor bone metastasis, which is also the leading cause of death from prostate cancer. The mechanism of bone metastasis of prostate cancer is not yet clear, but hematogenous dissemination is one of the important reasons. Studies at home and abroad have confirmed that the number of circulating tumor cells (CTC) in the blood of 50-70% of patients with prostate cancer metastasis is significantly increased. This phenomenon also suggests the great significance of CTC in clinical tumor metastasis research. A large number of studies have shown that circulating tumor cells in the peripheral blood of patients is closely related to poor clinical prognosis, and biopsy is an invasive examination that brings ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A01K67/027A01N1/02
CPCA01K67/0271A01K2207/12A01K2227/105A01K2267/03A01N1/0221C12N5/0693C12N2509/00
Inventor 张磊邵晨孙慧敏吴俊伸岳伦涛
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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