Method for extracting stigmasterol from coix stems

Abstract

The invention discloses a method for extracting stigmasterol from coix stems. The method comprises the following steps: enzymatic hydrolysis; ultrasonic extraction; saponification; urea column chromatography; crystallization; etc. The method provided by the invention can retain the natural characteristics of stigmasterol and has the advantages of few process steps, mild operation conditions, low solvent toxicity, safe production process and high extraction efficiency; and the extracted stigmasterol has a purity as high as 99%.

Description

technical field

[0001] The invention relates to the field of production and preparation of phytosterols, in particular to a method for extracting stigmasterol from coix stems. Background technique

[0002] Stigmasterol (stigmaster01), C 29 h 450 . The molecular weight is 412, also known as stigmaster-5,22-diene-3B-alcohol or 24R-ethylcholesteric-5,22-diene-3B-alcohol, which is a kind of phytosterol widely present in various in plants. Stigmasterol has significant anti-oxidation, anti-tumor, anti-cardiovascular disease and other pharmacological activities. It is also the starting material for the synthesis of steroid drugs and has applications in medicine, food and chemical industries. In recent years, with the discovery of new steroidal drugs, the development of production, and the expansion of production scale, the demand for raw materials has gradually increased; There is a contradiction between supply and demand, how to obtain more and higher purity stigmasterol is a...