Chromatographic Separation of Water-Soluble Organic Compounds and Determination of Benzene Ring Numbers in Components

Abstract

The invention provides a chromatographic separation and benzene ring number determination method for water-soluble organic matters. The method comprises the steps of preparing a water-soluble organic solution, adding the prepared water-soluble organic solution into a chromatographic column, subjecting the water-soluble organic solution to concentration / gradient elution through a fixed organic phase, detecting components obtained through the elution process by means of a fluorescence detector, and determining the number of benzene ring functional groups in each of different components according to the position of a maximum fluorescence peak in the emission spectrum of the separated components. The chromatographic separation and benzene ring number determination method has the advantages of small demanded sample quantity, good separation effect and accurate determination of benzene ring functional groups.

Description

technical field [0001] The invention belongs to the field of environmental analysis chemistry, and in particular relates to the chromatographic separation of water-soluble organic matter and a method for determining the number of benzene rings in the separated components. Background technique [0002] Water-soluble organic matter is a general term for a large class of organic mixtures, which contain various organic components. In order to accurately characterize its composition, researchers used various grouping techniques to separate and analyze its composition on the basis of reducing the complexity of organic matter composition. Currently commonly used grouping techniques include resin method, chromatography and grouping by molecular weight. Chromatographic grouping has become a common method for the separation and characterization of organic matter due to its advantages such as low sample requirement, fast separation speed, and online monitoring. [0003] In chromatogr...

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Problems solved by technology

Existing studies have focused on the fact that there are many hydrophobic organic substances in the environment, and the concentration range of organic solvent methanol or acetonitrile in the elution phase is 20% to 90%. Compared with hydrophobic organic pollutants in the environment, water-soluble organic substances are more hydrophilic. Strong, in the chromatographic separation process using the above ratio of organic solvents, the separation effect is not good

It is very important to determine the proportion of the organic phase in the chromatographic separation eluent. If the proportion of the organic phase in the eluent is too high, the peak time of different water-soluble organic components is close, and the separation effect is poor; if the proportion of the organic phase in the eluent is too low, the separation Long time and high column pressure reduce the service life of the separation column, so it is very important to reasonably determine the proportion of the organic phase in the eluent; in addition to the proportion of the eluent, the injection volume and sample concentration are also very important. Too little, the chromatographic detector cannot detect, the injection is too high, exceeds the detector range, and cannot effectively determine the content of different components

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