Rapid propagation method of high-quality seedlings of Dendrobium officinale with natural light cultivation for one-step seedling formation

Abstract

The invention discloses a rapid propagation method of high-quality seedlings of Dendrobium officinale with natural light cultivation for one-step seedling formation. The method mainly comprises the following six steps in order: a Dendrobium officinale material is prepared, cultivation rooms only with natural light are established, a fluid suspension of seeds is prepared, aseptic sowing is carried out for seeds, one-step seedling formation cultivation, and transplanting of test-tube seedlings. The one-step seedling formation cultivation is further divided into three phases: induced cultivation of protocorm, cultivation of small plantlets, and cultivation of big seedlings. The invention also comprises a one-step seedling formation medium used in the rapid propagation method. A one-step seedling formation is carried out without artificial transplantation, the cultivation rooms only with natural light are used, positions for cultivation can be changed according to different demands of light in different growth stages, and produced seedlings are healthy and strong as well as are easy to survive; special seedling exercising treatment is not needed before transplantation, after the seedlings are out of bottles, the seedlings can be transplanted directly, and production cost is substantially reduced. The seeds with high germination rate, medium with abundant nutrition and cultivation rooms only with natural light are used, in order to realize large scale production of one-step seedling formation of Dendrobium officinale seedlings.

Description

technical field [0001] The invention belongs to the plant regeneration technology through tissue culture technology, and in particular relates to a method for rapid propagation of high-quality dendrobium candidum seedlings cultured in natural light for primary seedling growth and the adopted primary seedling growth medium. Background technique [0002] Dendrobium officinale is an epiphyte of the genus Dendrobium orchidaceae, and it has been recognized as a precious Chinese herbal medicine since ancient times. The environmental conditions required for the growth and development of wild Dendrobium candidum are extremely harsh, with slow growth and extremely low reproductive rate, and human predatory mining has led to the near depletion of wild Dendrobium candidum resources. The current propagation methods are mainly divided into two categories, one is tissue rapid propagation. The seedlings should be hardened for 14 to 21 days before the planting of the tissue cultured seedli...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is the ubiquitous problems of long cultivation period, high pollution rate, complicated operation and high production cost of Dendrobium officinale seedlings. The present invention utilizes seeds with high germination rate and nutrient-rich medium , The full natural light cultivation room realizes the large-scale production of Dendrobium candidum seedlings at one time, which has the characteristics of low cost and high efficiency. It is a high-tech biotechnology for large-scale production of seedlings and can promote the sustainable development of Dendrobium candidum industry

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